Propargyl-PEG4-sulfonic acid

Name of Product

Lactobacillus/Pediococcus-Screen

Catalog Number

MGScLP

Short Info

This test discovers beer germs in a timely and precise manner

Method/Platform

PCR

Range/Assay Sensivity

10^4 – 10^5 cfu/mL

Test Principle

The technological basis for the GenLine tests  is the polymerase chain reaction (PCR) combined with lateral flow tests.

Labelled specific primers are used to amplify specific DNA fragments. In addition to the target gene, a control gene, which is also present in the PCR mixes, is amplified in order to make sure that the PCR process works properly.

The resulting PCR products carry the labels of the incorporated primers.

In a second part of the test, the created PCR products are detected by a lateral flow Test Strip.  A “molecular sandwich” is formed and becomes visible as a line on the test Strip.

Brief Instructions
The PCR reagents and the samples are prepared.
After the addition of the sample to the PCR reagents, the PCR is started.
The resulting PCR products are detected by a simple lateral flow test

Storage
-15 to -20°C

Components
PCR-Polymerase Master-Mix, PCR-Primermix, Positive Kit-Control, PCR-Water

N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.

N-(Propargyl-PEG2)-DBCO-PEG3-acid facilitates the creation of triazole linkages with azide-containing compounds using Click Chemistry. The carboxylic group can react with amine-containing entities using coupling agents like HATU for further conjugation.

Description

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications

Features

• Aptamer-based hot start PCR
• Reversible enzyme inactivation
• Omits extra enzyme activation step
• Convenient for room temperature PCR set-up
• High yield and specificity of target amplicons
• Wide range of amplicon length (up to 10 kb)
• High sensitivity (as low as 1 fg of plasmid)

Applications 

• High specificity PCR
• Generation of PCR products for TA cloning
• Routine PCR, multiplex PCR, colony PCR, and RT-PCR

Storage

-20°C for 24 months 

The ExcelTaq™ Hot Start II DNA Polymerase is a mixture of an aptamer-based inhibitor and a recombinant thermo-stable Taq DNA polymerase designed for preventing or minimizing non-specific DNA amplification in PCR reaction. The inactivation of polymerase is achieved by a reversible binding of the aptamer to the polymerase at temperatures below 45°C. The aptamer inhibitor releases polymerase during normal PCR cycling. The aptamer-based inhibition omits the time-consuming initial activation step required by chemically modified or antibody-based hot start polymerases.

The high specificity and sensitivity of ExcelTaq™ Hot Start II DNA Polymerase allows sensitive detection from limited amount of DNA templates, such as 1 pg of cDNA or 1 fg of plasmid DNA. With a high DNA synthesis rate and high thermo-stability, the ExcelTaq™ Hot Start II DNA Polymerase allows reactions to be set up at room temperature and is suitable for common and specialized PCR applications