Propargyl-PEG4-thiol

DBCO-Maleimide is a sulfhydryl reactive reagent containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety into cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-Maleimide is a sulfhydryl reactive reagent containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety into cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Rapid and efficient removal of abundant proteins from serum and plasma samples
• Process 10 samples in 30 minutes
• Allows for visualization of low abundance proteins
• Convenient and affordable spin column protocol
• Generic protocol allows for depletion from human and other animal samples
• Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

This kit provides a fast and simple procedure for the effective depletion of major serum proteins including albumin, α-antitrypsin, transferrin and haptoglobin from serum and plasma samples.  Such abundant proteins usually obscure less abundant proteins in gel electrophresis making them difficult to visualize and recover.  The kit reduces sample complexity to allow for convenient investigation of less abundant proteins.  In other applications such as mass spectroscopy, fractionating abundant proteins improves the resolution of less abundant proteins.  The kit is ‘generic’ in that it is not antibody-based and can thus be used to deplete abundant proteins from any starting material – human or various other animals – making it more versatile, flexible and affordable than other kits.   Resulting proteins are ready for a range of applications including 2D gel electrophoresis, SDS-PAGE, Mass Spectrometry, Protein microarrays, DIGE (Difference Gel Electrophoresis) and more.

Details

Supporting Data

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Kit Specifications
Maximum Column Capacity	500 μg
Minimum Column Capacity	200 μg
Minimum Elution Volume	30 μL
Time to Process 10 Samples	30 minutes

Storage Conditions
All solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.

Component	Cat. 17300 (25 preps)
Wash Solution G	125 mL
Elution Buffer C	8 mL
Protein Neutralizer	4 mL
Spin Columns	25
Collection Tubes	25
Elution Tubes (1.7 mL)	25
Product Insert	1