Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Different sizes:
Powerful magnets quickly secure beads.
Simply turn tube for efficient bead mixing.
The Primerdesign™ genesig® kit for Dengue subtypes genomes is designed for the in vitro
differentiation of Dengue subtypes (Dengue-1, Dengue-2, Dengue-3, and Dengue-4). The kit is designed to have the broadest detection profile possible whilst remaining specific to the individual Dengue subtypes.
The primers and probe sequences in this kit have 100% homology with a broad range of clinically relevant reference sequences based on a comprehensive bioinformatics analysis. They therefore have a very broad quantification profile.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
150 reactions
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and <50mg plant samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from 50mg plant using magnetic particles |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Plant and fungus samples |
| Sample amount | ≤50mg |
| Yield | 2-100μg |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R664101 | R664102 | R664103 |
| Purification Times | 48 Preps | 96 Preps | 480 Preps |
| MagPure RNA Particles | 1.7 ml | 3.5 ml | 18 ml |
| DNase I | 600 μl | 2 x 600 μl | 10 x 600 μl |
| DNase Buffer | 30 ml | 40 ml | 200 ml |
| Buffer PRC1 | 40 ml | 70 ml | 350 ml |
| Buffer RL | 40 ml | 70 ml | 350 ml |
| Buffer MCB* | 18 ml | 30 ml | 150 ml |
| Buffer MW1* | 22 ml | 44 ml | 220 ml |
| Buffer MW2* | 20 ml | 50 ml | 2 x 100 ml |
| RNase Free Water | 10 ml | 15 ml | 120 ml |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.
This product supplies a simple and rapid extraction of total RNA from plant and fungal samples. The kit is based on super paramagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction process takes only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure RNA Kits buffers can be used for both manual extraction process and automatic nucleic acid extraction machines. This Kits is suitable for extracting RNA from ≤5×106 cultured cells, 20mg tissue and
