DNA Concentrator (Magnetic Beads)

The DNA Concentrator (Magnetic Beads) can be used to efficiently concentrate DNA/RNA from various samples with low concentration without the need of a DNA vacuum concentrator. Solid Phase Reversible Immobilization (SPRI) magnetic beads are well used for DNA and RNA purification and concentration. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to DNA and RNA.

The concentrator protocol is simple: mix Concentrator Buffer and Concentrator Beads with the sample, wash, and elute pure DNA in a small volume to concentrate the DNA samples. Moreover, the DNA/RNA samples are also purified during the procedure. The beads with our unique technology purify DNA/RNA samples effectively by removing unwanted components such as dNTPs, enzymes, detergents, proteins, and other contaminants.

However, traditional magnetic beads can only bind nucleic acids that are 100 bp or longer. Nucleic acids shorter than 100 bp are not effectively recovered. We have successfully developed the kit that overcomes the hurdle of short nucleic acids recovery. The reagent can be used for concentration of samples from genomic DNA to short DNA/RNA.

Recovery rate

• >90% for DNA size >30 bp
• >80% for DNA size between 20-29 bp

Features

• Effective concentrator of DNA and RNA samples, even small DNA, RNA, oligos
• Binding capacity: 10 ug DNA per prep
• Removal of unwanted components and other impurities

The DNA Concentrator (Magnetic Beads) can be used to efficiently concentrate DNA/RNA from various samples with low concentration without the need of a DNA vacuum concentrator. Solid Phase Reversible Immobilization (SPRI) magnetic beads are well used for DNA and RNA purification and concentration. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to DNA and RNA.

Description

Specifications

Cytokeratin 18 (CK18) is present in simple, glandular, and transitional epithelial cells, but is absent in stratified epithelial cells. CK18 usually multimerizes with Cytokeratin 8, and Anti-Cytokeratin 18 is useful for detecting adenocarcinomas of simple and glandular epithelium origin, as well as poorly differentiated squamous carcinoma cells.