Propargyl-PEG7-NHS ester

Description

Tyrosinase Inhibitor Screening Kit (Colorimetric) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at OD = 510 nm. In the presence of kojic Acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased.

Tyrosinase or polyphenol oxidase is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling.

For rapid, sensitive and accurate screening of potential Tyrosinase inhibitors

N-(Acid-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the acid can be reacted with amines or alcohols to form stable amides or esters.

N-(Acid-PEG4)-N-bis(PEG4-Propargyl) is a trifunctional PEG linker that combines a t-butyl ester with two terminal alkynes. The alkynes can be applied in copper-click chemistry with azides to form stable triazole linkages with a target molecule, while the acid can be reacted with amines or alcohols to form stable amides or esters.

Overview

• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Ideal for use in in vitro diagnostic workflows
• Reliable and cost-effective
• Non-invasive, user-friendly sample collection
• Sample collection and preservation in one convenient kit
• Samples are non-infectious and can be handled and shipped safely
• Preserved DNA is stable for 2 years at ambient temperature
• High quality DNA is suitable for sensitive downstream applications including PCR, qPCR, sequencing, SNP analysis, microarrays, RFLP and Southern Blot Analysis
• Shipping accessories can be purchased separately

Norgen’s Saliva DNA Collection and Preservation Devices are designed for 1) simple and non-invasive saliva collection and 2) preservation of DNA in saliva samples at ambient temperature. Each of the 50 Saliva DNA Collection and Preservation Devices consists of 3 components: (1) Saliva Collection Funnel and Collection Tube, (2) Collection Tube Cap, and (3) Norgen’s Saliva DNA Preservative contained within a sealed squeezable ampoule. Saliva samples are collected by spitting inside the Collection Funnel which has been assembled with the Collection Tube. After collecting the required volume of saliva the Collection Funnel is removed and the contents of the Preservative Ampoule are then added and mixed with the collected saliva. The Saliva Collection Tube is subsequently sent to the laboratory for DNA isolation and analysis. DNA can be isolated from the preserved saliva samples using Norgen’s Saliva DNA Isolation Kit (Cat# Dx45400). Each of Norgen’s Collection Tubes is labeled with a unique serial number that can be used for secure and anonymous tracking of the sample. The saliva DNA in preserved samples is stable for more than 2 years at room temperature. This kit is ideal for collecting and preserving DNA samples for in vitro diagnostic use for medical purposes.

Saliva DNA Preservative

Norgen’s Saliva DNA Preservative is an aqueous storage buffer designed for rapid cellular lysis and subsequent preservation of DNA from fresh specimens. The buffer prevents the growth of Gram-negative and Gram-positive bacteria and fungi, and also inactivates viruses allowing the resulting non-infectious samples to be handled and shipped safely. In addition, the buffer eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperature. The components of the buffer allow samples to be stored for more than 2 years without any detectable DNA degradation.

DNA Isolation from Preservative

Prior to saliva DNA isolation, vortex the Collection Tube containing preserved saliva for 10 seconds and incubate at 55°C for one hour.  Saliva DNA can now be isolated from the preserved saliva samples using Norgen’s spin-column based Saliva DNA Isolation Kit Dx (Cat# Dx45400) or other commercially available methods that have been validated for use with Norgen’s Saliva DNA Collection and Preservation Devices.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

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Kit Specifications
Volume of Saliva Collected	2 mL
Volume of Saliva-Preservative Mix	4 mL
Preservation Temperature	Room Temperature
Preservation time	Over 2 years at room temperature

Shelf Life and Handling

• The Collection Device should be kept tightly sealed and stored at room temperature for up to 3 years without any reduction in kit performance (The collection due date is written on the device label).
• Once collected, saliva is stable for more than 2 years when kept tightly sealed and stored at room temperature.
• The Collection Tube, the Collection Funnel and the Device Container of each Individual Saliva DNA Collection and Preservation Device are recyclable.

Kit Components	Cat. 49000 (50 Devices)
Individual Saliva DNA Collection and Preservation Devices	50
Donor Procedure Flowchart	1
Product Insert	1
Individual Saliva DNA Collection and Preservation Device Contents
Saliva Collection Funnel and Collection Tube	1
Collection Tube Cap	1
Preservative Ampoule	1
Donor Instructions	1