Propargyl-PEG7-NHS ester

Compact and stylish designed, the BioShake iQ high-end thermoshaker lets you perform all your standard mixing runs with a minimum of adjustments.
The BioShake iQ offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.

Small. Light. Efficient. Quiet.

• For microplates, tubes and glass vials
• Fast & high-precision heating
• Temperature range: ambient to 99°C
• Precise mixing from 200 – 3,000 rpm
• Wide range of thermo adapters
• Stylish aluminium housing

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ADVANCED TECHNOLOGY

Save time.

By combining the mixing operation with the incubation phase, reaction process times and operator workload are reduced and efficiency of many procedures is increased, resulting in a higher throughput.

The first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.

GENTLE MIXING OF SAMPLES

By planar orbital motion

German designed and manufactured, BioShake iQ thermoshaker offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.

The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.

Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.

HOMOGENEOUS HEATING

Using large-area heating source in combination with digital sensor electronic

The BioShake iQ heats from ambient to 99°C in a short time, provides ease of use features, amazing comfort and maximum safety.

Within the BioShake iQ thinnest large-area heating elements and sensors are integrated. During the heat up time the special electronic control system guarantees the same temperature for all vessels with minimal deviations.
Temperatures are selectable from ambient temperature to 99°C in steps of 1.0°C. The nominal regulation accuracy is better than ±0.1°C with a uniformity of temperature distribution better than ±0.5°C at 45°C across the heating surface.

Exchangeable thermo adapter plates and thermal blocks enable a perfect adaptation to microplates, tubes or glass vials.

UNIVERSAL SPRING CLAMPS

Suitable for all microplates

In the corners eight spring clamps are inserted for easy fixation of skirted microplates and deep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.

For flexible and deformable PCR plates we recommend using of the related PCR adapter.

Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.

COMFORT

Simple intuitive operation and programming

The BioShake iQ is operated from the front face of the enclosure which includes touch buttons and a display. 

Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.

The short mix button allows short and fast mixing in between.

The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time, mixing frequency and temperature.

EXCHANGABLE THERMO ADAPTERS

For a wide range of applications

The BioShake iQ comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.

Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels. An excellent temperature uniformity and homogeneity is guaranteed for all samples.

UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY

First-class finished aluminium housing

Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.

Safety of the user and ease of use are clearly a priority.

MADE IN GERMANY

High-end instruments for daily routine

A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.

The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.

For microplates, tubes and glass vials
Fast & high-precision heating
Temperature range: ambient to 99°C
Precise mixing from 200 – 3,000 rpm
Wide range of thermo adapters
Stylish aluminium housing

Overview

• Excellent separation and purification of cytoplasmic and nuclear RNA
• Convenient and fast spin column format
• High quality and yield of RNA
• Isolate full diversity of RNA (including microRNA) without phenol
• Purified RNA is ready for any application including RT-PCR, qRT-PCR, RNA-Seq, arrays and more
• Cytoplasmic RNA is free of DNA and ready for direct use in RT-PCR/qRT-PCR
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit provides a rapid method for the isolation and purification of both cytoplasmic and nuclear RNA from cultured animal cells and small tissue samples. The kit can be used to isolate all sizes of RNA from the cytoplasmic and nuclear RNA fractions, including all small RNA species without any requirement for phenol.   Included in the kit are sufficient reagents to perform either 50 cytoplasmic RNA preparations or 25 cytoplasmic and 25 nuclear RNA preparations.   Ten samples can be processed in approximately 45 minutes.  This kit is also available in a 100 prep size.    

Background

In certain circumstances it is desirable to be able to isolate fractionated RNA as opposed to total RNA. For example, it may be preferable to isolate only mature, cytoplasmic RNA for some studies on expression profiling. Alternatively it may be desirable to isolate nuclear RNA in order to investigate and study pre-processed (non-spliced) RNA. Furthermore, this kit can be used to isolate RNA for downstream applications where it is necessary to avoid DNA contamination, since the cytoplasmic fraction has been shown to be free of all traces of genomic DNA. 

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	Up to 50 µg RNA
Maximum Column Loading Volume	650 µL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Time to Complete 10 Purifications	45 minutes
RNA Yield HeLa (1 x 106) – Cytoplasmic RNA HeLa (1 x 106) – Nuclear RNA	15 µg ≤ 3.5 µg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 21000 (50 preps)	Cat. 37400 (100 preps)
Lysis Buffer J	20 mL	2 x 20 mL
Buffer SK	40 mL	2 x 40 mL
Wash Solution A	38 mL	2 x 38 mL
Elution Buffer E	6 mL	2 x 6 mL
Mini Spin Columns	50	100
Collection Tubes	50	100
Elution Tubes (1.7 mL)	50	100
Product Insert	1	1

Product Description

Amp-Future NFO Isothermal Amplification Kit, -20℃ Storage, 48 Tests/Box, 500-1000copies/µL Detection Limit, 5-20mins Test

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit NFO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(NFO type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months