Propargyl-PEG7-t-butyl ester

Description

The Plant RNA Kit (NA2014-01) is optimized for purification of total RNA from a wide variety of plant samples which rich in polysaccharides and phenol. It comes with proprietary magnetic beads and a specially optimized buffer. Toxic solvents such as phenol, chloroform are not needed in the process. The eluted RNA is high-quality and is ready for all downstream applications, such as RT-PCR, Northern blotting, differential display. The procedures can be fully automated on the magnetic particle processor instrument and ease of use.

Overview

• Extract high quality & quantity total RNA including miRNA 
• Isolate from a wide variety of animal tissues 
• No phenol step required – isolate all RNA in one fraction
• Compatible with tissues stored in RNAlater® or Trizol®  
• Convenient & fast spin column format
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc.  Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

Figure 1 / 4

Previous

Next

Click for expanded view

Kit Specifications
Maximum Column Binding Capacity	50 μg
Maximum Column Loading Volume	650 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 10 Purifications	50 minutes
Average Yield Liver (10mg) Kidney (10mg) Spleen (10mg) Heart (10mg) Muscle (10mg)	30 μg 25 μg 15 μg 10 μg 5 μg
Maximum Amount of Starting Material    Heart Kidney Liver Muscle  Spleen	30 mg 15 mg 15 mg 30 mg 15 mg

Storage Conditions and Product Stability
The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.

Component	Cat. 25700 (50 preps)
Buffer RL	30 mL
RNase-Free Water	40 mL
Wash Solution A	38 mL
Enzyme Incubation Buffer A	6 mL
Elution Solution A	6 mL
Proteinase K	2 vials
DNase I	1 vial
Mini Spin Columns	50
Collection Tubes	100
Elution Tubes (1.7 mL)	50
Product Insert	1