Propargyl-PEG8-acid

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Nineteen discrete fragments ranging from 100 bp to 10000 bp
• Higher intensity reference bands at 500 bp, 1000 bp and 5000 bp

The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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HighRanger Plus 100 bp DNA Ladder (Cat# 12000) – 100 loads

Ladder Properties:

• Nineteen discrete fragments ranging from 100 bp to 10000 bp
• Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	10000	42
2	8000	33
3	6000	25
4	5000	42
5	4000	35
6	3000	27
7	2500	22
8	2000	18
9	1500	13
10	1000	40
11	900	28
12	800	25
13	700	34
14	600	19
15	500	44
16	400	13
17	300	18
18	200	13
19	100	10

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
This kit is stable for 2 years after the date of shipment.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

N-(Propargyl-PEG4)-biocytin is a biotin PEG reagent that is reactive with azide containing molecule via click chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Propargyl-PEG4)-biocytin is a biotin PEG reagent that is reactive with azide containing molecule via click chemistry. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Aminooxy-PEG4-propargyl is a aldehyde reactive crosslinker. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.

Aminooxy-PEG4-propargyl is a aldehyde reactive crosslinker. The aminooxy group reacts with an aldehyde or ketone group to form a stable oxime linkage. The propargyl group is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries. Aminooxy compounds are very reactive and sensitive; they cannot be stored for long term. Immediate use (within 1 week) is highly recommended.