Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG8-t-butyl ester is a PEG reagent with an alkyne group and a t-butyl group. The alkyne group enables the formation of triazole linkage with azide-bearing compounds or biomolecules; copper is required as a catalyst. The t-butyl group can be hydrolyzed under acidic conditions. The hydrophicility of the molecule is improved by having 8 units of PEG. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Specifications
| Features | Specifications |
| Main Functions | Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant. |
| Applications | RT-PCR，PCR |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant |
| Sample amount | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
Kit Contents
| Contents | IVD6672-50 | IVD6672 |
| Purification Times | 50 Preps | 200 Preps |
| Bead Tube C | 50 | 4 x 50 |
| MagPure Particle | 1.6 ml | 7.0 ml |
| Proteinase K | 24 mg | 100 mg |
| Protease Dissolve Buffer | 1.8 ml | 6 ml |
| Buffer MLBN | 30 ml | 120 ml |
| Buffer MW1* | 22 ml | 53 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Buffer NFW | 10 ml | 30 ml |
Storage and Stability
MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
DBCO-NHCO-PEG5-NHS ester is a PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-NHCO-PEG5-NHS ester is a PEG linker containing NHS ester that is able to react specifically and efficiently with primary amines such as the side chain of lysine residues or aminosilane-coated surfaces at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and can also provide a long and flexible connection that minimizes steric hindrance during ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The Norgen LowRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our LowRanger contains eleven discrete fragments ranging from 100 bp to 2000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for fast running times and accurate visual determination.
Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
Figure 1 / 1
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LowRanger 100bp DNA Ladder (Cat# 11500) – 100 loads
Ladder Properties:
• Eleven discrete bands, ranging from 100 bp to 2000 bp
• Higher intensity band at 500 bp for easy reference.
| Fragment | Size (bp) | Mass (ng) |
| 1 | 2000 | 110 |
| 2 | 1500 | 83 |
| 3 | 1000 | 55 |
| 4 | 800 | 44 |
| 5 | 700 | 39 |
| 6 | 600 | 33 |
| 7 | 500 | 55 |
| 8 | 400 | 22 |
| 9 | 300 | 17 |
| 10 | 200 | 22 |
| 11 | 100 | 22 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
Stable at room temperature. For longer term storage, -20°C is recommended.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
