Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG9-alcohol is a crosslinker that can participate in copper catalyzed azide-alkyne Click Chemistry reactions to form stable triazole linkage. The PEG spacer increases the hydrophilicity of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)
Product Info
Document
Product Info
Product Details
Application:
DNA Nucleic Acid
Format:
NFO Kit
Kit Components:
Reagents,Buffer,Enzymes
Manufacturer:
Amp-future Bio
Product Name:
DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)
Reaction Time:
5-20mins
Reaction Volume:
50μL
Sensitivity:
500-1000copies/ml
Shelf Life:
14 Months
Specificity:
High
Storage Conditions:
-20℃
Suitability:
Universal
High Light:
Isothermal DNA Amplification Kit
,
DNA Amplification Kit NFO
,
20mins nucleic acid amplification
Payment & Shipping Terms
Minimum Order Quantity
48T
Price
USD$3.8/T
Packaging Details
16T/Bag,48T/Box
Delivery Time
6 working days
Payment Terms
T/T, MoneyGram
Supply Ability
100000T/Month
Product Description
DNA Isothermal Rapid Amplification Kit (Colloidal Gold Test Strip Type)
【Principle Overview】
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
【Primer design】
It is recommended to use primers with a length of 30-35 bp.Too short primers will affect the amplification speed and detection sensitivity;the 5′ end of the reverse primer is labeled with a modification group (commonly used biotin).
Primers are designed to avoid the formation of secondary structures that affect amplification; the length of the amplicon is recommended to be 150-500bp.
【Colloidal gold probe design】
In the middle of the forward and reverse primer,design a sequence of 46-52nt in length that is complementary to the target fragment; modify an antigen marker (typical FAM) at the 5′ end;mark a dSpacer (tetrahydrofuran, THF) in the middle of the 5′ end and the 3′ end ), as the recognition site of nfo;the 3′ end is labeled with a modification group, such as amine group, phosphate group or C3-Spacer, etc.
【Features】
This kit has the advantages of high sensitivity, strong specificity,and short reaction time (only 15 minutes),and the reaction components are in dry powder state,which is easy to operate and easy to store.
This reagent has low equipment requirement,and the reaction operation can be carried out in metal baths, water baths, etc.,and there is no need to purchase expensive exclusive equipment such as PCR amplifiers.
Composition
Content
A buffer
1.6mL×1Tube
B buffer
150μL×1Tube
Positive control template-II
100μL×1Tube
Positive control probe and primer mix-II
70μL×1Tube
Reagent
48 T
Guide Manual
1 Copy
【Kit storage】
1. Storage conditions: storage temperature ≤ -20℃constant temperature environment, keep away from light and avoid heavy pressure;
2. Product validity period: 14 months;
3. See the outer packaging for the production date.
Document
This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins, the recombinase and primers form a complex; Source search and combine the target homology domain, at this time, a D-loop region is formed at the homology position and strand exchange begins; along with the dissociation of the recombinase from the complex, the polymerase also binds to the 3′ end of the primer and begins chain extension. Relying on the action of nfo enzyme, adding specific molecular probes designed according to the template, and using colloidal gold technology (sandwich method) can detect the final result.
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
L-Malic Acid
Assay Format:
Spectrophotometer, Microplate
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Increase
Linear Range:
0.5 to 30 µg of L-malic acid per assay
Limit of Detection:
0.25 mg/L
Reaction Time (min):
~ 3 min
Application examples:
Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK
The K-LMAL-58A pack size has been discontinued (read more)
L-Malic Acid (Regular) Assay Kit, for the specific assay of L-malic acid (L-malate) in beverages and food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Need other assay kits? View our full list of organic acid assay kits.
Advantages
PVP incorporated to prevent tannin inhibition
Both enzymes supplied as stable suspensions
Very competitive price (cost per test)
All reagents stable for > 2 years after preparation
Very rapid reaction (~ 3 min)
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Compact and stylish designed, the BioShake XP high-speed microplate mixer lets you perform all your standard mixing runs with a minimum of adjustments. The XP offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing
ADVANCED TECHNOLOGY
Compact & stylish designed
Mixing tasks in microplates can be realized fast & safe with using adjustable speed of 200 up to 3,000 rpm.
The BioShake mixing-technology is obviously more robust, vibration free and needs less maintenance compared to classical mixers.
The first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake XP plate mixer offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
COMFORT
Simple intuitive operation and programming
The BioShake XP is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time and mixing frequency.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority for us.
EXCHANGABLE ADAPTERS
For a wide range of applications
The BioShake XP comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
Document
Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing