Propargyl-PEG9-amine has a propargyl group and an amine group. The amine group reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) to form amide bonds. The propargyl group reacts with azides to form triazole linkage with the presence of copper as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG9-amine has a propargyl group and an amine group. The amine group reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) to form amide bonds. The propargyl group reacts with azides to form triazole linkage with the presence of copper as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Norgen’s TaqMan 2x PCR Master Mix is a ready-to-use TaqMan 2x PCR Master Mix solution that contains a PCR internal control which can be detected by HEX/VIC channel in a real-time PCR machine. By detecting the internal control users can validate the DNA template quality, thereby preventing any false negatives in the PCR results. The user needs only to add template, target TaqMan primer/probe mix and water to set up the TaqMan real-time PCR.
PCR Control: TaqMan 2x PCR Master Mix contains PCR control primers/probe (HEX/VIC) and PCR control template. The PCR control reaction in the TaqMan 2x PCR Master Mix is optimized to not interfere with target amplification. The fluorescence of the target probe should not be HEX/VIC.
Details
Reagents Supplied – Cat # 28340
TaqMan 2x PCR Master Mix (3 Vials, 100 Reactions) – Sufficient reagent for 100 x 20 µL reactions
Storage Conditions and Product Stability Norgen’s TaqMan 2x PCR and 2x RT-PCR Master Mixes should be stored at -20ºC. For everyday use an aliquot can be stored at 4ºC for up to three months. The Master Mix is stable for multiple freeze-thaw cycles. When stored at the proper temperature this reagent is stable for at least 1 year.
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution
Applications
PCR, sequencing, labeling reactions, ligations and restriction digestion, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Crude DNA, enzymatic reaction solution
Sample amount
<150μl
Recovery
80%
Elution volume
≥15μl
Time per run
≤10 minutes
Liquid carrying volume per column
800µl
Binding yield of column
20µg
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
High recovery efficiency – up to 80% DNA recovery
Fast – isolation can be completed in 10 minutes by column gel method
Low elution volume – elution volume can be as low as 10μl
Efficient – efficient removal of various inhibitory factors
Kit Contents
Contents
D214102
D214103
Purification Times
100 Preps
250 Preps
Buffer GWP
30 ml
60 ml
Buffer DW2
20 ml
50 ml
Elution Buffer
30 ml
60 ml
HiPure DNA Mini Columns I
50
250
2 ml Collection Tubes
50
250
Storage and Stability
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
Document
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.