Propargyl-PEG9-bromide consists of a propargyl group and a bromide group. The propargyl group reacts with azide compounds in copper catalyzed Click Chemistry reactions. The bromide (Br)can be used as a leaving group for nucleophilic substitution reactions. The 9-unit PEG spacer improves the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Propargyl-PEG9-bromide consists of a propargyl group and a bromide group. The propargyl group reacts with azide compounds in copper catalyzed Click Chemistry reactions. The bromide (Br)can be used as a leaving group for nucleophilic substitution reactions. The 9-unit PEG spacer improves the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
AAV Purification Kits
Product Info
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Product Info
Overview
AAV Purification from any input – cell fraction or media fraction
High AAV recovery, up to 90%
No specialized equipment needed
Purification from a variety of AAV serotypes (including AAV6 and AAV9)
Yields highly active AAV for in vivo and in vitro experiments
Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
Recombinant adeno-associated virus (AAV) vectors are highly promising tools for both in vitro and in vivo gene transfer. Norgen’s AAV Purification Kits provide fast and simple procedures for concentrating and purifying AAV vectors from cell lysate and cell culture media. Purification is based on precipitation onto Norgen Biotek’s proprietary resin. Contaminating cellular debris is largely removed from the sample via a centrifugation step, while contaminating DNA and RNA is reduced using enzymatic digestion. AAV vector purified in this manner is highly active for use in in vitro and in vivo transduction experiments.
AAV Purification Kit
Norgen’s AAV Purification Kit contains sufficient materials for 15 preparations (33.5 mL per prep of supernatant (SN) or a total of 500 mL of supernatant input). Approximately 1 mL of cell pellet can be purified per prep, up to a maximum of 15 mL of cell pellet in total for the entire kit. Up to 33X sample concentration.
AAV Purification Mini Kit
Each spin column is able to concentrate and purify AAV from 0.5-8 mL of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (200 µL). Preparation time for 4 samples is 1.5 hours, with 45 minutes of hands-on time.
AAV Purification Midi Kit
Each spin column is able to concentrate and purify AAV from 8 mL up to 45 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 50X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1 mL). The kit may be used to purify up to 8 x 25 mL or 4 x 45 mL of samples using the included columns. Preparation time for 4 samples is approximately 2 to 2.5 hours, with 1.5 hours of hands on time.
AAV Purification Maxi Kit (Slurry Format)
Each spin column is able to concentrate and purify AAV from 45 mL to 90 mL of input consisting of cell pellet, cell culture media, or cells and culture media mixed together. Up to 200X sample concentration. AAV vector purified in this manner is highly active for use in in vitro transduction experiments, and is eluted into a small volume (1-10 mL) using the optional concentration step. The kit may be used to purify up to 1 x 900 mL samples or 10 x 45-90 mL samples using the included columns. Preparation time for 1 x 900 mL sample is approximately 2.5 to 3.5 hours, with an optional concentration step requiring an additional 30 min.
At least 5 x 1010 AAV particles as determined by qPCR
AAV Vector Serotype
AAV6, AAV9 and others
Input Type
Cells, media
Input Volume (AAV supernatant)
1 – 33.5 mL SN per prep (500 mL SN in total)
Input Volume (AAV cell pellet)
1 mL cell pellet per prep (15 mL in total)
Time to Complete Purifications
2.5 to 4.5 hours with 1 hour hands on time
In vivo transduction
Yes
Storage Conditions and Product Stability
HL-SAN Nuclease should be stored at -20°C upon arrival. Elution Buffer O should be stored tightly capped at 4°C upon arrival. All other solutions should be kept tightly sealed and stored at room temperature. Once opened, the solutions should be stored at 4°C. This kit is stable for 2 years after the date of shipment.
Component
Cat. 66100 (15 preps)
Cat. 63200 (20 preps)
Cat. 63300 (4-8 preps)
Cat. 63250 (1-10 preps)
Lysis Buffer S
5.5 mL
5.5 mL
5.5 mL
20 mL
DNAse I
–
2 x 25 uL
2 x 25 uL
210 μL
RNAse A
–
60 μL
60 μL
240 μL
HL-SAN Nuclease
102 μL
–
–
–
Binding Buffer A
20 mL
4 mL
4 mL
2 x 8 mL
Purification Solution C
60 mL
–
–
–
Purification Solution D
130 mL
–
–
–
Wash Solution C
2 x 130 mL
60 mL
60 mL
3 x 60 mL
Slurry E
12.5 mL
–
–
2 x 14.5 mL
Elution Buffer O
66 mL
8.5 mL
8.5 mL
66 mL
Protein Neutralizer
4 mL
4 mL
4 mL
4 mL
Spin Columns
–
20
–
–
Mini Spin Columns
–
20
–
–
Midi Spin Columns (grey contents) with Collection Tubes
–
–
8
10
Midi Spin Columns (white contents) with Collection Tubes
–
–
8
–
Maxi Spin Columns (grey contents) with Collection Tubes
–
–
–
10
Maxi Spin Columns (white contents) with Collection Tubes
Attogene’s Montmorillonite Bentonite Clay binds toxins with high affinity and high capacity. Montmorillonite has been shown to be the active ingredient in bentonites. The proposed mechanism of action of Attogene’s Montmorillonite Bentonite Clay is through the adsorption of toxins (mainly onto the interlayer spaces of montmorillonite). Attogene’s Montmorillonite Bentonite Clay is a common anti-caking agent in animal feeds to adsorb aflatoxins and diminish their bioavailability. Its composite for application in drug system can be formed through the utilization of anionic, cationic, and nonionic surfactants.
Bentonite is a promising rock of clay which is found in nature. It is a major source of montmorillonite in nature. It is a rock produced of highly colloidal and plastic clays mainly comprised of montmorillonite. In addition to montmorillonite, bentonite may compose of some amount of crystalline quarz, cristobalite, and feldspar. Montmorillonite nanoclay is used as a drug carrier system and as an additive. Its composite for application in drug system can be formed through the utilization of anionic, cationic, and nonionic surfactants. These are used to improve basal spacing resulting in organo clay to be utilised in drug loading and drug release.
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Promotes flocculation of cyanobacteria cell debris, binds residual phosphate compounds and promotes gradual settling of the cyanobacterial biomass on the sediment.
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Details
Specifications
Features
Specifications
Main Functions
Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP)
Applications
NGS, DNA library
Purification technology
Magnetic beads technology
Process method
Manual (centrifugation or vacuum)
Sample type
DNA products, restriction endonuclease systems, or other enzymatic reaction solutions
Sample amount
Appropriate
Recovery
80%
Operation time
≤50 minutes
Principle
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
High recovery – up to 80%
High throughput – using magnetic beads purification technology
Kit Contents
Contents
BP-5
BP-50
BP-500
MagPure A4 XP
5 ml
50 ml
500 ml
Storage and Stability
MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
Document
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.