Propargyl-PEG9-bromide

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target Accurate controls to confirm findings

Description

The genesig® Real-Time PCR COVID-19 (CE) is CE-IVD marked and intended for in vitro diagnostic use in Europe.

PLEASE NOTE: This is a Professional Use Only product that requires a trained operator in a controlled laboratory environment. It is NOT a lateral flow or a Point of Care device designed to be used by the general public.

Total workflow solution
All reagents and components provided from start to finish
Rapid protocol with fewer handling steps
For use with dry swabs
Viral inactivation; Preparation, Amplification, Diagnosis
Total chemistry optimisation
Low shipping costs with ambient shipping
Includes our genesig® Real-Time PCR Coronavirus COVID-19 (CE IVD)

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Ten discrete fragments from 100 bp to 1000 bp
• Higher intensity reference band at 500 bp

The Norgen PCRSizer 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our PCRSizer contains ten discrete fragments ranging from 100 bp to 1000 bp in 100 bp increments with a higher intensity reference band at 500 bp. This Ladder is ideal for PCR product size confirmation.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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PCR Sizer 100 bp DNA Ladder (Cat# 11400) – 100 loads

Ladder Properties:
• Ten discrete bands, ranging from 100 bp to 1,000 bp
• Higher intensity band at 500 bp for easy reference

Fragment	Size (bp)	Mass (ng)
1	1000	78
2	900	70
3	800	62
4	700	55
5	600	47
6	500	78
7	400	31
8	300	24
9	200	31
10	100	24

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.