Attogene’s Protein Biotinylation kit provides the user with reagents for labeling proteins
Desalting columns for purifying the labeled molecule
Convenient and easy to interpret instructions on how to carry out the labeling process.
Made by Attogene in the USA
The kit provides rapid, simple, and reliable components for the labeling proteins with biotin for use in lateral flow procedures. Kit enables the biotinylation of antibody or proteins to be used with the Universal Lateral Flow Kits (AU2034, AU2037 or AUAU2044 in addition to Streptavidin Colloidal (AU2017) gold or Streptavidin CdZnS Quantum Dots (AU2043).
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from insect tissue |
| Applications | PCR, southern bolt and virus detection, etc |
| Purification method | 96 well DNA plate |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Insect tissue samples |
| Sample amount | |
| Elution volume | |
| Time per run | |
| Liquid carrying volume per column | |
| Binding yield of column |
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
| Contents | D313901 | D313902 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure DNA Plate | 1 | 4 |
| 2.2 ml Collection Plate | 1 | 4 |
| 1.6 ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| Seal Film | 8 | 32 |
| Buffer ITL | 30 ml | 120 ml |
| Buffer IL | 30 ml | 125 ml |
| Buffer GW1 | 44 ml | 2 x 110 ml |
| Buffer GW2 | 50 ml | 3 x 50 ml |
| Proteinase K | 50 mg | 200 mg |
| Protease Dissolve Buffer | 6 ml | 15 ml |
| Buffer AE | 20 ml | 60 ml |
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in thiscase buffers should be redissolved before use. Make sure that all buffers areat room temperature when used.
HiPure Insect DNA Kits provides a simple and rapid solution for total DNA extraction of insect tissue samples. This kit is based on silica gel column purification technology without toxic phenol chloroform extraction and time-consuming alcohol precipitation. The whole extraction process only takes 30 minutes. HiPure Insect DNA Kit can process tissue samples less than 10mg at a time. Hipure Insect DNA 96 kit can process 96 insect tissue samples at a high throughput. The obtained DNA can be directly used in PCR, Southern blot, viral DNA detection and other experiments.
GL20 Technical Parameter：
| Max. Speed | 21000rpm |
| Max. RCF | 30910×g |
| Max. Capacity | 4×800ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 60 |
| Temperature | -20℃~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/110V 50HZ/60HZ |
| Size (W x D x Hmm) | 680×540×830mm |
| Net Weight(Kg) | 170KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for GL20
| Order No | Rotor | Rotor Max Speed(r/min) | Volume(ml) | RCF(×g) |
| G20-1 | Swing Rotor | 4000 | 4×800ml | 3450 |
| G20-2 | Microplate rotor | 4000 | 4×4×96 well | 2940 |
| G20-3 | Microplate rotor | 4000 | 2×4×96 well | 3210 |
| G20-4 | Microplate rotor | 4000 | 2×3×48 well | 2300 |
| G20-5 | Swing rotor | 4000 | 4×40×7ml vacuum tube | 3270 |
| G20-6 | 4000 | 4×40×5ml vacuum tube | 2890 | |
| G20-7 | 4000 | 4×28×10ml vacuum tube | 3130 | |
| G20-8 | Swing Rotor | 4000 | Fat bottle | 3830 |
| G20-9 | Fixed Rotor | 16000 | 4×8PCR | 15760 |
| G20-10 | Fixed Rotor | 15000 | 6×8PCR | 21420 |
| G20-11 | Fixed Rotor | 16000 | 8×8PCR | 17480 |
| G20-12 | Fixed Rotor | 15000 | 12×8PCR | 22930 |
| G20-13 | Fixed Rotor | 15000 | 40×0.5ml | 22920 |
| G20-14 | Fixed Rotor | 21000 | 12×1.5ml | 30910 |
| G20-15 | Fixed Rotor | 16000 | 24×1.5ml | 23440 |
| G20-16 | Fixed Rotor | 14000 | 30×1.5ml | 20800 |
| G20-17 | Fixed Rotor | 13000 | 48×1.5ml | 17930 |
| G20-18 | Fixed Rotor | 16000 | 16×5ml | 22020 |
| G20-19 | Fixed Rotor | 16000 | 6×10ml | 21500 |
| G20-20 | Fixed Rotor | 15000 | 12×10ml | 22680 |
| G20-21 | Fixed Rotor | 13000 | 16×10ml | 19490 |
| G20-22 | Fixed Rotor | 13000 | 8×15ml | 17790 |
| G20-23 | Fixed Rotor | 11000 | 12×15ml | 14330 |
| G20-24 | Fixed Rotor | 5000 | 24×15ml | 3500 |
| G20-25 | Fixed Rotor | 5000 | 30×15ml | 3830 |
| G20-26 | Fixed Rotor | 14000 | 6×30ml | 19060 |
| G20-27 | Fixed Rotor | 13000 | 6×50ml(conical) | 18840 |
| G20-28 | Fixed Rotor | 13000 | 6×50ml(round) | 18730 |
| G20-29 | Fixed Rotor | 5000 | 12×50ml | 3860 |
| G20-30 | Fixed Rotor | 4000 | 24×50ml | 2970 |
| G20-31 | Fixed Rotor | 13000 | 4×85ml | 18940 |
| G20-32 | Fixed Rotor | 12000 | 4×100ml | 14850 |
| G20-33 | Fixed Rotor | 10000 | 6×100ml | 11380 |
| G20-34 | Fixed Rotor | 4000 | 12×100ml | 2970 |
| G20-35 | Fixed Rotor | 12000 | 24 capillaries | 15800 |
| G20-36 | Swing Rotor | 15000 | 4×5ml | 19920 |
| G20-37 | Vertical rotor | 16000 | 16×5ml | 16450 |
| G20-38 | Vertical rotor | 14000 | 8×30ml | 19750 |
Features
1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control
3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.
4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.
5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.
6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.
7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.
DBCO-PEG24-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG24-NHS ester is a click chemistry PEG reagent containing NHS ester that is able to react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. The hydrophilic PEG spacer arm improves water solubility and provides a long and flexible connection that minimizes steric hindrance involved with ligation. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
