The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method) developed under patent by Medallion Labs. This digestibility score, in conjunction with the essential amino acid profile plus protein and moisture content of the sample, is used to calculate the Protein Digestibility Corrected Amino Acid Score (PDCAAS) value.
Detail
K-PDCAAS
SKU: 700004324
50 assays per kit
Content:
50 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Protein Digestibility
Assay Format:
Spectrophotometer
Detection Method:
Absorbance
Wavelength (nm):
570
Signal Response:
Increase
Total Assay Time:
~ 6 h (over 2 days)
Application examples:
Protein containing foods and feeds.
Method recognition:
Novel Method (Under Patent: U.S. Pat. No. 9,738,920)
The Protein Digestibility Assay Kit (K-PDCAAS) is used for in vitro measurement of the Animal-Safe Accurate Protein Quality Score (ASAP-Quality Score Method) developed under patent by Medallion Labs. This digestibility score, in conjunction with the essential amino acid profile plus protein and moisture content of the sample, is used to calculate the Protein Digestibility Corrected Amino Acid Score (PDCAAS) value.
The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
Qubit tubes from the same lot should be used for the experiments for optimal result. 500 tubes per package.
Features
High-quality: Accurate measurement of different types of samples
Cost cutting: Half price as compared to other vendors
Specifications
Shipping condition
Room temperature
Type
Assay tube (for Qubit fluorometer)
For use with (equipment)
Qubit fluorometer
Storage
Store at room temperature
Package
500 tubes
Qubit is a registered trademark of ThermoFisher Scientific.
Document
The Qubit assay tubes are thin-wall polypropylene tubes (500 µl) for use with the Qubit Fluorometer. High-quality of the tubes guarantee the accurate measurement of different types of samples such as DNA (dsDNA, ssDNA), RNA and protein.
The Genomic DNA Extraction Kit (HMW, Magnetic Beads) provides a reliable and fast process for extracting high molecular weight (HMW) genomic DNA from cells, blood, and tissues using Solid Phase Reversible Immobilization (SPRI) magnetic beads. With our proprietary magnetic beads technology, the kit eliminates the tedious centrifuge steps for columns. The kit provides a reliable and simple approach for high-quality genomic DNA isolation with fast magnetic response time and high binding capacity.
Cat.# 50014 Genomic DNA Extraction Kit for Cells (HMW, Magnetic Beads) Cat.# 50015 Genomic DNA Extraction Kit for Blood (HMW, Magnetic Beads) Cat.# 50016 Genomic DNA Extraction Kit for Tissues (HMW, Magnetic Beads)
The extracted HMW genomic DNA size ranges are dependent on the beads resuspension: 50-150 kb by tube tapping and 40-100 kb by tube vortexing. Purified DNA is recovered at high yield and high purity without RNA contamination. The typical purity ratios of A260/A280 are around 1.8-2.0, and A260/A230 are around 2.2-2.5. Purified HMW genomic DNA is suitable for applications such as long-read sequencing, linked-read genome assembly, long range PCR, optical mapping, and other general applications.
Features
High molecular weight DNA: 50 kb to 150 kb
High purity
Simple magnetic beads method
No centrifuge needed
No column needed
No vacuum needed
A portion of the extracted genomic DNA samples were loaded on a PFGE gel with a DNA ladder indicated. Sample A: liver tissue; Sample B: intestine tissue; Sample C: whole blood; Sample D: cultured 293T cells.
Cultured Cell samples
Cultured cells are collected and are resuspended in a buffer and then lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in the Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Blood samples
Whole blood is resuspended in the RBC buffer to remove RBC. The remaining leucocytes are lysed with a lysis buffer, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Tissue samples
Tissues are homogenized and lysed, then mixed with beads to bind genomic DNA. The samples are mixed with a buffer and after washing steps, genomic DNA is eluted in Elution Buffer. The isolated genomic DNA with the magnetic beads is free of contamination such as RNA, proteins, salts, and other impurities.
Attogene’ s RNase Inhibitor is a protein-based ribonuclease inhibitor which noncovalently binds and inactivates a wide variety of RNases in a range of temperature (37–65°C) and pH (5.5–8.5) conditions. This product is distinct from placental RNase Inhibitor protein in that it inactivates RNases I and T1 in addition to RNases A, B and C. NA2021 is distinct from RNase Inhibitor protein in that it is less expensive, have more robust interactions with RNAses.
MADE AND FUNCTIONALLY TESTED AT ATTOGENE (MADE IN AUSTIN TEXAS – USA)
Document
RNase Inhibitor (10,000 units)
Concentration: 40 units/μL
Volume: 0.25 mL
Storage Conditions: Store at 2°C – 8°C
Inhibits: RNases A, B, and C
Supplied in: 1X PBS with 0.05% Sodium Azide.
