[QP2310] Q-PAGE™ Bis-Tris Precast Gel (Mini, 12 wells, 12%), 10 gels

Gel images of different ranges of library size selection. Sheared human genomic DNA was used as input.

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Library size selection is an enrichment of a specific range of library sizes for NGS library preparations. The NGS library preparation is related to the quality of the sequencing data. Precise NGS library size selection can increase sequencing efficiency, improve data quality, and reduce costs.

There are two types of sequencing technologies: short-read sequencing and long-read sequencing. Short-read sequencing uses DNA libraries that contain small insert DNA fragments of similar sizes, usually several hundred base pairs. The sequencing efficiency can be improved if the DNA size selection is in the right range. Cat.# 20104S and 20104L are the best kits for NGS library size selection of illumina paired-end 100 (PE100) sequencing with 100-200 bp library inserts; Cat.# 20105S and 20105L are the best kits for NGS library size selection of illumina paired-end 150 (PE150) sequencing with 150-300 bp library inserts; and Cat.# 20106S and 20106L are the best kits for NGS library size selection of illumina paired-end 300 (PE300) sequencing with 300-600 bp library inserts.

Long-read sequencing uses a large DNA fragment as input and makes very long reads. Usually, library size selection is preferred to remove smaller fragments. Cat.# 20110S and 20110L are the best kits for long-read sequencing size selection with DNA sizes >5 kb, and Cat.# 20111S and 20111L are the best kits for long-read sequencing size selection with DNA sizes >10 kb.

The magnetic beads, or SPRI (Solid Phase Reversible Immobilization) beads, is well used for the purification of DNA due to their reversible DNA binding. The NGS library can be size-selected by the magnetic beads or SPRI beads. The properties of the magnetic beads can be changed for a specific range of DNA binding. The contaminants and other unwanted components in the libraries can also be removed during size selection.

Specific ranges of NGS libraries can be selected using magnetic beads with different buffer compositions. The first DNA-beads binding step, also called the right-side clean-up, removes large NGS library fragments. The large NGS library fragments that bind to the beads are discarded with the beads pellet. The desired NGS library fragments in the supernatant are transferred to a new well, and new beads are added to the supernatant for the second beads-DNA binding, also called the left-side clean-up. After the rinsing step, the NGS library fragments with the dual selection are eluted in water or an appropriate buffer. The magnetic beads method has great advantages over time-consuming column purification and tedious gel-based purification.

NGS library size selection with dual clean-ups.

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Library size selection for long-read sequencing only requires a single clean-up. In this case, only the large library fragments are bound to the beads, while other small library fragments are discarded with the supernatant. The selected larger library fragments are eluted in water or an appropriate buffer after the rinsing step.

NGS library size selection with single clean-up for >5 kb and >10 kb libraries.

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Features of NGS library size selection

• • • 5 ranges for NGS library size selection
      • • illumina PE100 sequencing: 250-350 bp
        • illumina PE150 sequencing: 300-450 bp
        • illumina PE100 sequencing: 450-750 bp
        • Long-read sequencing: >5 kb
        • Long-read sequencing: >10 kb
    • Rapid protocol: only 20 minutes
    • Simple workflow
      • • No columns required
        • No gel purification required
        • No centrifugation required

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Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Histamine
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	492
Signal Response:	Increase
Linear Range:	0.5 to 12 μg of histamine per assay
Limit of Detection:	~ 0.09 μg of histamine per test or ~ 0.75 mg/Kg (ppm) of histamine in a fish sample
Limit of Quantification:	~ 0.5 mg of histamine per test or  ~ 4.2 mg/Kg (ppm) of histamine in a fish sample
Reproducibility (%):	~ 3%
Reaction Time (min):	~ 20 min
Application examples:	Fish samples in various forms (e.g. fresh, canned, frozen, etc).

This product has been discontinued.

The Histamine Assay Kit for the detection of histamine in food including fish samples in various forms.

High levels of histamine may develop in a variety of foods as they decompose, this is an issue that particulary affects fish.

Megazyme’s Histamine Assay Kit provides a simple robust method with a fast reaction time of ~ 20 mins.

View all of our assay kits.

Advantages

• Very competitive price (cost per test)
• All reagents stable for > 2 years
• Specific for histamine
• Suitable for use with raw fish samples
• Simple format. Well suited to automation
• Standard included
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing

The Histamine Assay Kit for the detection of histamine in food including fish samples in various forms.

SD-25/SD-25C Vacuum Centrifugal Concentrator

Ambient temperature type/Refrigerated type

Technical Parameter

Temperature range	Room Temperature~+80℃	-10℃~+80℃
Temperature Accuracy	士1℃
Max.Speed	2500rpm
Max.Capacity	6x100ml
Timer	0~99h59min
Vacuum control range	0.1~1000mbar
Main unit withstands vacuum levels	0.01mbar
Noise	≤55dBA	≤65dBA
Voltage	0.7kw	1.3kw
Power supply	220v,50Hz
Dimension(L×W×Hmm)	360*448*304mm	440*653*420mm

Adapter Rotor

Rotor Type	Rotor Volume	Rotor Type	Rotor Volume
Angle Rotor	42×1.5/2ml	Angle Rotor	8×50ml
Angle Rotor	108×1.5/2ml	Angle Rotor	6×100ml
Angle Rotor	48×5ml	Angle Rotor	16*20ml Sample vial
Angle Rotor	30×10ml	Angle Rotor	16*5ml+8*4ml+8*3ml
Angle Rotor	24×15ml	Microplate Rotor	2×96well

Key features：
1. High-definition LCD touch screen, simultaneously displaying operational parameters: speed, temperature, vacuum, and running time.
2. Delayed start: after reaching a fixed speed, Start the vacuum pump to effectively prevent sample mixing.
3. Fully automatic vacuum control system, vacuum setting range0.1~1000mbar, Control accuracy0.1mbar.
4. 1.5/2ml Rotor stacking for simultaneous use; rotor replacement without tools.
5. Programmable with temperature and pressure settings; capable of storing 36 programs
6. Compatible with various rotor heads; transparent glass window for easy observation.
7. Magnetic drive: maintenance-free, non-contact drive, fully sealed.
8. Safety in use: inductive electric self-suction door lock ensures the centrifugal concentrator can only start when the door cover is fully closed and locked.
9. Can be used in conjunction with freeze-drying systems
Model:
SD-25/SD-25C

Brand:
Yingtai