Introduction
This kit is suitable for extracting total pathogen nucleic acid from a variety of clinical samples such as blood, serum, plasma, swab soaking solution, fluid accumulation and homogenate solution. This kit is designed to remove host cells background nucleic acid and enrich pathogen nucleic acid (including viral/bacterial/fungal DNA/RNA) from the sample. Purified DNA/RNA is ready for downstream applications such as PCR, virus detection, tNGS and other related experiments.
Details
Specifications
| Features | Specifications |
| Main Functions | Extract Pathogen RNA/DNA from 0.5-1.5ml whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution for tNGS application, remove host background nucleic acid. |
| Applications | Real Time PCR, biochip analysis, NGS |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | whole blood, plasma, serum, body fluid, homogenate suspension, culture solution, cell suspension, soaking solution or concentrate pathogen solution |
| Sample amount | 0.5 – 1.5 ml |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Kit Contents
| Contents | R667200C | R667202C |
| Purification Times | 24 Preps | 96 Preps |
| 2ml Bead Tube (0.4g) | 24 | 96 |
| DNase I (Powder) | 10 mg | 15 mg |
| DNase Buffer | 5 ml | 20 ml |
| Protease Dissolve Buffer | 3 ml | 8 ml |
| Lysis Buffer LBX1 | 40 ml | 180 ml |
| Buffer TL | 5 ml | 20 ml |
| Proteinase K | 24 mg | 120 mg |
| MagBind Particles N9 | 1.2 ml | 5 ml |
| Buffer MLB | 30 ml | 120 ml |
| Buffer MW1* | 13 ml | 110 ml |
| Buffer MW2* | 10 ml | 50 ml |
| Buffer AVE | 10 ml | 20 ml |
Storage and Stability
Proteinase K, DNase I powder and MagPure Particles N9 should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
