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The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
Detail
Description
Features of the resDNASEQ CHO Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL.
The recovery rate of different concentration samples in the linear range is between 70% and 130%.
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Other Products
Laboratory Square Cavity Front Door Freeze Dryer/ Lyophilizer
Product Info
Document
Product Info
Techinical parameter
ltemNo
SH7006
SH9006
SH9009
Ice condenser capacity
6kg
6kg
9kg
Ice condenser temperature
-70℃
-90℃
-90℃
Chamber volume
12L
15L
Freeze dried chamber
Square cavity/front door opening/door opening angle up to 180 degrees
Sample shelf
·Stainless steel template partition● Electric heating/optional
Vacuum pump
Extreme vacuum5*10mbar ,with a pumping capacity of 8m³/hbuilt-in to the host/optional for various types of pumps
Oil mist filter
Standard configuration
Vacuum pump connection pipe
Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents
Anti-corrosive treatment
Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
>The display can simultaneously display:all main parameters such as cold trap temperature,vacuum degree,sample temperature, and shelf temperature.
>Schematic diagram of equipment operating components,through graphic display to view the working status of each component of the equipment.
>Ithas the functions of cold trap precooling and vacuum pump preheating,and the time can be set by the userin the system. >Display whether the current running status is normal or not by numerical color.
o One-key operation,the operation is extremely simple,no special professional learning and training is required.
o Thermal electromagnetic over-current and short-circuit protection,making the equipment run more safely and stably.
o Active reminder of equipment maintenance time and vacuum pump replacement lubricating oil time.
o Ambient temperature monitoring,the device automatically reminds the alarm if the ambient temperature is too high.
o Optional eutectic point detection device and endpointjudgment detection system make freeze-drying more scientific.
Dryingchamber
· The separate drying chamber reduces the probability of damage caused byimproper handling of the drying chamber due to height and weight.The upper cover is anodized aluminum with anti-corrosion treatment.●Highly transparent plexiglass drying chamber,good light and heat radiation conduction.Reserve 6/12 external valve ports.· 316L stainless steel all black Teflon coating drying chamber,suitable for drying organic solvents and various corrosive samples,reserved for 6/12 external ports.· 316L stainless steel vertical multi-port multi-manifold external rack(The standard configuration is aplexiglass drying chamber,and one ofthe diying chambers can be optional)
Coldtrapandcondensingcoil
· The cold trap cavity and condensation coil are made of 316L stainless steel.Large opening and channel design,the gas can be quickly captured through the condensing coil.· The cavity and condensing coil are equipped with Teflon anti corrosion coating as standard,which can trap organic solvents and various corrosive solvents.· The condensing coil is placed in the cold trap to increase the condensate catchment area,which can effectively prevent external condensation.
Sampleshelves
· 316Lstainless steel stackable sample shelves,capable oflyophilizing machine solvents and various corrosive solvents.Each shelf can be placed and taken freely without manual work.The distance and height ofeach shelfcan be adjusted freely.-All black coating,good heat absorption performance.-Special hole for freeze-drying of centrifuge tubes,which is conducive to the drying of samples in centrifugetube containers.· All-aluminum Teflon surface anti-corrosion treatment electric heating shelf.
Refrigerationsystem
· Danffos compressorCopper plate heat exchanger,the largest area to increase heat dissipation· Unique refrigeration technology,the temperature of the cold trap can be lowered from ambient temperatureto below-80C°within 2 minutes.· Class A temperature sensor with high temperature accuracy and low error.· Multiple detection and alarm system,the refrigeration system pressure is too high or refrigeration failure cantimely alarm and take corresponding measures.
Techinical parameter
ItemNo
ST7003
ST7006
ST9006
Ice condenser capacity
3kg
6kg
6kg
Ice condenser temperature
-70℃
-70℃
-90℃
Cold trap volume
5L
10L
10L
Freeze dried chamber
Bell shape (standard configuration)/Multi manifold type/T-shaped frame type
Sample shelf
φ200mm.Single layer area 0.03m2 with 3-5 layers available
●260mm/485mm.Single layer area 0.055m²/0.185m².3-10 layers/optional ● Electric heating/manual capping/optiona
Vacuum pump
Extreme vacuum 5*10*mbar with a pumping capacity of 8m³/h optional pump of various types
Oil mist filte
Standard configuration
Vacuum pump connection pipe
Single forming stainless steel flexible pipe DN16 ISO-KF L-1.2m
Anti-corrosive treatment
Cold trap,condensing coil/all equipped with PTFE anti-corrosion treatment as standard. Freeze-drying organic solvents.
External valve
6pieces/optional
1-48 pieces/optional
Eutectic point detection system
Support/Optional
Data output analysis
Support/Optional
Powe
0.6kw
1.1kw
1.5kw
Size
420*545*455mm
510*570*500mm
Document
Freezer Dryer, 3kgs-6kgs water capturer
Model:
ST7003,ST7006,ST9006
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells,
12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
The product is made of medical grade USP CLASS VI polymer polystyrene
The product is made under a 100,00- class dust-free manufacturing site
Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
Gamma radiation sterilization
Non-Pyrogenic, DNase/Rnase free.
Document
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
