GL12 Technical Parameter：

Matched Rotors for GL12

Features

1. Widely used in the field of biochemistry, biological products, pharmaceutical factory and laboratory.

2. Brushless frequency motor for model GL12 which in great torque, free maintenance, no powder pollution, quick in speed up and down.

3. Model GL12 with the digital display which indicates the speed、time and temperature.

4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.

5. There are high speed rotors and low speed rotors for your choice.

6. Electric lid lock, compact design, super speed and imbalance protection.

7. The centrifuge body is made of high-quality steel, safe and reliable.

Model:
GL12

Brand:
Yingtai

Code:
8421199090

Plasmid MiniPrep Kit & High Throughput Kit (Magnetic Beads)

The kits were developed for plasmid miniprep directly from bacterial cultures. 100% centrifuge-free; NO vacuum needed; No column needed.

Plasmid MiniPrep Kit (Magnetic Beads), Cat.# 50012
– Use 2 ml of bacteria culture directly

Plasmid MiniPrep High Throughput Kit (Magnetic Beads), Cat.# 50011
– High throughput: Use 0.2 ml of bacteria culture directly with 96-well plates
– Low throughput: Use 0.2 ml of bacteria culture directly with 1.5 ml tubes

The kits can be used for both high copy and low copy numbers of plasmid DNA. With BioDynami’s proprietary magnetic beads technology, the kits eliminate the requirement of centrifuge, vacuum, and column. Our magnetic beads provide a robust and reliable tool for both high throughput and low throughput applications of plasmid DNA isolation with high binding capacity and fast magnetic response time.

The High Through kit (Cat.# 50011) can be used for both high throughput and low throughput extraction of plasmid DNA. Up to 96 samples can be extracted simultaneously when using a 96-well plate.

Yield of plasmid DNA may vary dependent on the bacteria strain, plasmid type, copy numbers, and growth conditions etc. DNA extracted using the kit is suitable for downstream applications such as qPCR, PCR, DNA sequencing, molecular cloning, restriction enzymatic digestion, transfection, and transformation etc. The isolated plasmid DNA with the magnetic beads is free of contaminations such as RNA, bacterial DNA, proteins, and other impurities.

Plasmid DNA were loaded on 1% of agarose gel after extraction.

Comparison of workflows of Magnetic Beads based kits

Features

• 100% centrifuge-free
  • • Culture medium can be used directly without centrifuge to pellet the bacteria
• Simple
  • • No centrifuge
    • No column
    • No vacuum
• Flexible applications with simplified miniprep protocol
  • • High throughput: 96-well plates with 0.2 ml of each culture (Cat.# 50011)
    • Low throughput: 1.5 ml tubes with 0.2 ml of each culture (Cat.# 50011)
    • Low throughput: 1.5 ml tubes with 2 ml of each culture (Cat.# 50012)
    • Ideal for screening and other applications

Introduction

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.

Details

Specifications

Principle

The HiPure silica gel column uses a high binding ability glass fiber filter membrane as the substrate. Under the condition of high concentration of ionizing agent (such as Guanidinium chloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bonding and electrostatic and other physical factors, while protein or other impurities are not adsorbed and removed. The filter membrane that has adsorbed nucleic acids is washed to remove proteins and salts. Finally, low salt buffer solution (such as Buffer TE) or water can be used to wash out the nucleic acids adsorbed on the filter membrane. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The stool samples are homogenized in the lysis solution, further lysed in a high-temperature water bath, and RNA is released into the lysis solution. Chloroform extraction removes genomic DNA and impurities, transfer the supernatant to an alcohol free binding solution, purify RNA through a column, and finally elute RNA with RNase Free Water. The purified RNA can be directly used for experiments such as PCR, Southern hybridization, and enzyme digestion.

Advantages

• High purity – unique adsorbent for more efficient removal of inhibitors
• High concentration – maximum extraction of total RNA from stool samples
• Sensitive – RNA can be purified at the level of PG

Kit Contents

Storage and Stability

The kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions. At low temperatures, Buffer SPL may form precipitates, dissolve it by 55°C water bath. After receiving the product, Buffer PHC should be stored at 2-8°C.

Hipure Stool RNA Kit is specially designed for stool RNA extraction. This kit is suitable for extracting high-purity microbial or host cell RNA from ≤0.1g stool samples. The kit adopts silica gel column purification technology and original solution system, which can effectively remove humic acid and other inhibitory factors in stool samples. The purified RNA can be directly used in RT-PCR, Northern hybridization and other experiments.