RPA/MIRA is used for DNA and RNA nucleic acid templates isothermal amplification , and can be used in the field of molecular detection of viruses, pathogenic bacteria, tissues, cells, etc.
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Payment & Shipping Terms
Packaging Details
16T/Bag,48T/Box
Delivery Time
6 working days
Payment Terms
T/T, MoneyGram
Supply Ability
100000T/Month
Product Description
RPA/MIRA is used for DNA and RNA nucleic acid templates isothermal amplification , and can be used in the field of molecular detection of viruses, pathogenic bacteria, tissues, cells, etc.
MIRA VS PCR ,MIRA Advantages:
Low and constant temperature, low requirements on instruments
20mins
Only one pair of primers is needed
Fluorescence detection by probe method is also available
High specificity
PCR :Need to control temperature,90 minutes
LAMP:Design three pairs of primers,60minutes,Low specificity
Other Products
Propargyl-PEG4-bromide
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Product Info
Propargyl-PEG4-bromide is a crosslinker that can be used in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage with azides. The bromide (Br) acts as a good leaving group for nucleophilic substitution reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG4-bromide is a crosslinker that can be used in copper catalyzed azide-alkyne Click Chemistry to form a stable triazole linkage with azides. The bromide (Br) acts as a good leaving group for nucleophilic substitution reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Preservation and isolation of both cf-DNA and cf-RNA from a single tube
Fixative-free preservative, no cross-linking of DNA
Preserve cf-DNA/ct-DNA for 30 days at ambient temperature and for up to 8 days at 37°C
Preserve cf-RNA for 30 days at ambient temperature
Preserve Circulating Tumour Cells (CTCs) for 14 days at ambient temperature
No plasma volume loss after shipping/transportation
Prevent hemolysis allowing better separation of plasma
Prevent apoptosis of blood cells and fragmentation of genomic DNA
Produce high quality/quantity of plasma cf-DNA/ct-DNA/cf-RNA
Vacuumed to draw 8.7 mL of blood in 10 mL tubes
The procedure for plasma isolation using this kit is compliant with ISO 20186-3:2019
Norgen’s cf-DNA/cf-RNA Preservative Tubes are closed, evacuated plastic tubes for the collection and the preservation of cf-DNA, circulating tumor DNA, cf-RNA and circulating tumor cells in human whole blood samples during storage and shipping. Using these tubes in combination with Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits enables the purification and concentration of an inhibitor-free cf-DNA/ct-DNA/cf-RNA in a very small elution volume. Plasma recovered from Norgen’s cf-DNA/cf-RNA Preservative Tubes is also compatible with any other cf-DNA and/or cf-RNA purification method. The purified cf-DNA, ct-DNA and cf-RNA are compatible with any downstream application assays, including PCR, qPCR, rt-qPCR, methylation-sensitive PCR, Southern Blot analysis, gene expression analysis, microarrays and NGS.
This product is for research use only and not for use in diagnostic procedures.
Performance
Norgen’s cf-DNA/cf-RNA Preservative Tubes abridge the collection/processing of whole blood for the subsequent purification of cf-DNA and/or cf-RNA. The cf-DNA/cf-RNA Preservative Tubes preserve and inhibit the programmed cell-death (apoptosis) of the blood, hence preventing the release of intracellular DNA/RNA into plasma. Whole blood samples can be shipped and stored at room temperature for the subsequent processing of plasma. The cf/ct DNA and cf-RNA levels are stable for up to 30 days at room temperature. The cf-DNA is also stable for up to 8 days at 37°C. When blood collected on Norgen’s cf-DNA/cf-RNA tubes is processed for plasma recovery no buffy coat will be generated and the Circulating tumor cells (CTCs) will be located at the bottom of the tube with the blood sediment. CTCs are stable for 14 days at room temperature where it can be extracted from the blood sediment or from whole preserved blood for cell sorting or for cf/DNA or cf-RNA purification.
Plasma samples produced from the whole blood sample collected into Norgen’s cf-DNA/cf-RNA Preservative Tubes and purified using Norgen’s Plasma/Serum Cell-Free Circulating DNA Purification Kits or Norgen’s Plasma/Serum RNA Purification Kits yield concentrated, high-quality and inhibitor-free cf-DNA/ct-DNA or cf-RNA for any downstream application.
Workflow
NOTE: This product is for research use only. For the CE marked version, see Cat. Dx63950
Up to 30 days at room temperature (15-25°C) Up to 8 days at 37°C
Length of cf-RNA Preservation
Up to 30 days at room temperature (15-25°C)
Length of CTCs Preservation
Up to 14 days at room temperature (15-25°C)
Storage Conditions and Product Stability
When stored at 15-30°C, unfilled Norgen cf-DNA/cf-RNA Preservative Tubes are stable through the expiration date. See tube label for expiry date.
Short-term storage/shipping at -20°C to 37°C for up to 10 days is acceptable for unfilled Norgen cf-DNA/cf-RNA Preservative Tubes.
Do not freeze unfilled Norgen cf-DNA/cf-RNA Preservative Tubes for long term storage over 10 days. Freezing unfilled tubes may lead to loss of vacuum and precipitation may occur.
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
Cell-free DNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 8 days when stored at 37ºC.
Cell-free RNA in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes is stable for up to 30 days when stored at room temperature (15-25°C)
Circulating tumor cells (CTCs) in blood samples collected in Norgen’s cf-DNA/cf-RNA Preservative Tubes are stable for up to 14 days at room temperature (15-25ºC).
Urine DNA Isolation Kit for Exfoliated Cells or Bacteria
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Overview
Isolate genomic DNA from either exfoliated cells or bacteria found in urine
Isolate and detect genomic DNA from as little as 1 mL of urine and up to 50 mL urine
High quality DNA for sensitive applications
Rapid processing time
Norgen’s Urine DNA Isolation Kit for Exfoliated Cells or Bacteria is designed for the rapid isolation of either: 1) human genomic DNA from exfoliated cells that have been shed into the urine from the urinary tract; or 2) bacterial genomic DNA from urine samples. The kit allows for the isolation of DNA from 1 to 50 mL of urine. The genomic DNA isolated from exfoliated cells can be used in a number of diagnostic and research applications including the diagnosis and monitoring of bladder, kidney, or other urinary-tract cancers. Bacterial genomic DNA from both human urine samples and urine samples from animals can be isolated with this kit in order to study the levels and types of bacteria that are present. The kit allows for the isolation of genomic DNA from both Gram negative and Gram positive bacteria, including E. coli, Proteus spp., Klebsiella spp., Enterobacter spp., Serratia spp., Pseudomonas spp, Clostridial ssp. and Leptospirosis spp.Chlamydia trachomatis and Neisseria gonorrhoeae.
Typical yields of human genomic DNA from exfoliated cells will vary depending on the cell density of the urine sample, which is affected by a number of factors including health, diet and sex of the individual donating the urine. Typical yields of bacterial genomic DNA will vary depending on the urine sample and the bacterial species, if any, present in the urine. Healthy humans generally have < 10, 000 CFU of bacteria per mL of urine, and this kit is sufficiently sensitive to isolate and detect DNA from even this minimal amount of bacteria. The genomic DNA purified with this kit is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications such as PCR, qPCR and Southern Blot analysis.
10 minutes (Plus a 30 minute incubation – Bacteria) (Plus a 15 minute incubation – Exfoliated)
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 2 years after the date of shipment when stored at room temperature.
