RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.
Detail
RNA into DNA and PCR in one step? Then, this enzyme will simplify PCR analysis from RNA templates reducing labor and time. RT-KTQ2 was evolved from the thermostable KlenTaq DNA polymerase with no significant reverse transcriptase activity. Four mutations ensure that the variant is reverse transcriptase active and even PCR active, while maintaining the thermostability. This allows to perform reactions at high temperatures minimizing problems encountered with strong secondary structures in RNA that melt at elevated temperatures. For further information refer to the original publication.
Available upon request and for R&D use only – Contact Us
RT-KTQ2 DNA polymerase is supplied as a 5 µM solution containing glycerol and is supplied together with 10x reaction buffer.
The enzyme can also be used for real-time cycling, when adding a suitable dye.
Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
The Animal Disease Kit includes multiple components, such as primers, probes, reagents, buffers, etc., to detect diseases. The kit offers various detection methods, including test strips, making it suitable for a broad range of applications in animal disease detection.
Animal Disease Kit is widely used in cattle veterinary medicine, animal disease detection, and animal feed. The kit has been proved to be reliable and efficient in the detection of animal diseases. It helps to diagnose diseases quickly, providing an accurate basis for subsequent treatments.
Support and Services:
We are committed to providing quality products and services to our customers. Our Animal Disease Kit is designed for veterinary professionals to diagnose and treat medical conditions in animals.
1.Technical Support
Our technical support staff are available to answer any questions you may have about the Animal Disease Kit. We provide assistance with product installation, troubleshooting, and any software or hardware issues you may encounter. We also offer technical advice on how to use the kit and its features.
2.Customer Service
Our customer service team is available to help you with any inquiries you may have about the Animal Disease Kit. We assist with product returns, replacement parts, and warranty information. We also provide product information and advice on how to get the most out of your Animal Disease Kit.
FAQ:
Q1: What is the “Animal Disease Kit” product?
A1: The “Animal Disease Kit” is a comprehensive toolkit for the diagnosis of animal diseases.
Q2: What does the Animal Disease Kit include?
A2: The Animal Disease Kit includes Primers, Probes, Reagents, Buffers, Etc.
Q3: How do I use the Animal Disease Kit?
A3: The Animal Disease Kit is designed for easy and convenient use. You can refer to the instructions manual for detailed instructions on how to use the product.
Q4: How accurate is the Animal Disease Kit?
A4: The Animal Disease Kit is designed for accurate and reliable results. It has been tested and certified by qualified professionals for accuracy and reliability.
Q5: Is the Animal Disease Kit safe to use?
A5: Yes, the Animal Disease Kit is safe to use. All components of the kit are made with non-toxic and non-irritating materials, and all reagents are safe to use.
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Animal Disease Kit is a diagnostic kit designed for animal detection. It is suitable for the detection of various diseases, including cat and dog test kits, in animals. The kit is composed of various components such as primers, probes, reagents, buffers and other materials necessary for the detection of diseases, which can be used for test strips and other detection methods. The kit can be stored at -20°C for long-term use. With this Animal Disease Kit, you can easily detect diseases in animals and protect them from harm.
NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform)
Product Info
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Product Info
The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.
NGS DNA Fragmentation & Library Prep Kit Workflow
The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C.
Kit features:
Fast: 1-hour library construction from intact genomic DNA to NGS library
Total time: 1 hr
Hands-on time: 5 min
Intact genomic DNA can be used directly as input; DNA fragmentation is not required.
Works with both EDTA-free DNA samples and DNA samples in TE buffer
Simple workflow
Three steps
Only one beads purification step
Guaranteed quality: high library conversion efficiency based on our chemistry
Library conversion efficiency for NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform). 100 ng, 300 ng and 500 ng of DNA were used as input.
NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform): Library size distribution with different incubation time.
Document
The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.
