SNPsig® SARS-CoV-2 (20J/501Y.V3)

Overview

• CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
• Available for sale in Canada (Dx17200, Dx24300)
• Ideal for use in in vitro diagnostic workflows
• Extract high quality & quantity total RNA including miRNA
• No phenol step required
• Bind & elute all RNA irrespective of size or GC content, without bias
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Available in 96-Well and 96-Well (Deep Well) formats for high throughput automation
• Isolate from a wide variety of specimens
• Purified RNA is suitable for a variety of downstream applications
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Kits Dx provide rapid methods for the isolation and purification of total RNA from tissue samples, blood, plasma, serum, saliva, bacteria, yeast, fungi and viruses for subsequent in vitro diagnostic use. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

Total RNA Purification Kit Dx (Spin Column)

This kit is designed to be used with any downstream application employing enzymatic amplification or other enzymatic modifications of RNA followed by signal detection or amplification. Any diagnostic results generated using the RNA isolated with Norgen’s Total RNA Purification Kit Dx in conjunction with an in vitro diagnostic assay should be interpreted with regard to other clinical or laboratory findings.

Total RNA Purification 96-Well Kits Dx (High Throughput and High Throughput Deep Well)

Thess 96-well kits provide a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Intended Use

To minimize irregularities in diagnostic results, suitable controls for downstream applications should be used. Norgen’s Total RNA Purification Kits Dx are intended for use by professional users such as technicians, physicians and biologists experienced and trained in molecular biological techniques. Norgen’s Total RNA Purification Kits Dx do not provide a diagnostic result. It is the sole responsibility of the user to use and validate the kit in conjunction with a downstream in vitro diagnostic assay.

NOTE: This product is not available for sale in the United States.

Details

Supporting Data

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Kit Specifications
Maximum Binding Capacity Per Well	50 μg
Maximum Loading Volume Per Well	500 μL
Size of RNA Purified	All sizes, including small RNA < 200 nt
Time to Complete 96 Purifications	30 minutes
Average Yield HeLa Cells (1 x 106 Cells) E. coli (1 x 109 cells) Brain (10 mg) Liver (10 mg) Blood (50 μL, Hamster)	15 μg 50 μg 9 μg 26 μg 1 μg
Maximum Amount of Starting Material:  Whole Blood Plasma/Serum Bacteria Yeast Fungi Plant Tissue	100 µL150 µL1 x 109 cells1 x 108 cells 40 mg 40 mg

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature.  These reagents should remain stable for at least 1 year in their unopened containers.

Component	Cat. Dx17200 (50 preps)	Cat. Dx24300 (192 preps)	Cat. Dx24350 (192 preps)	Cat. Dx24380 (576 preps)
Lysis Solution	40 mL	–	–	–
Buffer RL	–	2 x 40 mL	2 x 40 mL	350 mL
Wash Solution	22 mL	–	–	–
Wash Solution A	22 mL	2 x 38 mL	2 x 38 mL	1 x 148 mL 1 x 74 mL
Elution Solution	6 mL	–	–	–
Elution Solution A	–	2 x 20 mL	2 x 20 mL	60 mL
Mini Spin Columns	50	–	–	–
96-Well Isolation Plate	–	2	2	–
96-Well Isolation Plate (Deep Well)	–	–	–	6
Collection Tubes	50	–	–	–
96-Well Collection Plate	–	2	2	–
96-Well Collection Plate (Deep Well)	–	–	–	6
Elution Tubes (1.7 mL)	50	–	–	–
96-Well Elution Plate	–	2	2	–
96-Well Elution Plate (Deep Well)	–	–	–	6
Adhesive Tape	–	4	4	12
Product Insert	1	1	1	1

• Non-specific amplification is prevented by using this Taq DNA polymerase variant as the DNA polymerase is hotstart-formulated. The 10x reaction buffer supplied together with the DNA polymerase has been specifically designed for optimal PCR performance and DNA polymerase activity. This allows the use of this DNA polymerase in a wide range of PCR applications.For research use and further manufacturing. Designed and manufactured under ISO13485. Please contact us for bulk quantities and quotes.

Broad Amplification Range

Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.

Faster Detection and Higher Sensitivity

A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.

Non-specific amplification is prevented by using this Taq DNA polymerase variant as the DNA polymerase is hotstart-formulated. The 10x reaction buffer supplied together with the DNA polymerase has been specifically designed for optimal PCR performance and DNA polymerase activity. This allows the use of this DNA polymerase in a wide range of PCR applications.