SR0030 Novobiocin

Product Description

High Sensitivity RNA Amplification With Improved Detection Isothermal Kit

Product Detail

Applicable equipment:

It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature: at room temperature and constant temperature (generally 39℃~42℃), with the help of auxiliary proteins and single-strand binding proteins,the recombinase and primers form a complex;Source search and combine the target homology domain, at this time,a D-loop region is formed at the homology position and strand exchange begins;along with the dissociation of the recombinase from the complex,the polymerase also binds to the 3′ end of the primer and begins chain extension. At the same time, relying on the function of exonuclease, adding specific molecular probes designed according to the template, and using fluorescence monitoring equipment can realize real-time monitoring of the amplification process of the target fragment.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

Technical Parameters:

Parameters	Details
Product Name	DNA Isothermal Amplification Kit EXO
Manufacturer	Amp-future
Storage Temperature	-20°C
Kit Components	Enzymes, Buffers ,Reagents
Packaging	48 Tests/box
Detection Limit	500-1000copies/µL
Shipping	ICE
Test Time	5-20mins

Isothermal nucleic acid Applications

Suitable for DNA isothermal rapid amplification kit(fluorescent type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

Fluorescent Probe:Require the suitable length is 46-52nt.

DNA fluorescent kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

It is recommended to use the Isothermal fluorescence detector developed by Amp-future, which is also suitable for fluorescence quantitative PCR apparatus with market known brands.

For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.

About

3CR Bio’s Leaf Sample Collection Kit – Your Gateway to High-Quality DNA Extraction and Genotyping Lab Services

The correct sampling and storage of leaf samples for DNA extraction have a significant impact on the final quality and quantity of the DNA extracted. Our simple, lightweight kits provide everything you need to sample and ship your leaf tissue from anywhere in the world, so it reaches us in top condition.

Use the 3CR Bioscience Leaf Sample Collection Kit to sample and prepare leaf tissue optimally when shipping to us for DNA extraction and Genotyping. Full instructions available in the Leaf Sample Collection User Guide.

Kit Contents

• 1 x 96-tube storage rack containing individual 2D-barcoded tubes sealed with a secure locking lid.
• 1 x Sponge pad to fit inside the lid of the storage rack for shipping.
• 1 x Sealed black plastic packet containing 2 x 10g sachets of desiccant.
• 1 x Large clear sealable plastic bag.
• 1 x Basic leaf cutting tool and leaf cutting mat (order separately)

or

• 1 x Advanced cutting tool (order separately)
• Plate Map for recording sample IDs (image not shown, provided in paper and electronic form)

The Plant Kit is used in combination with our Lab Services.
Visit the Lab Services page for more information about our DNA Extraction, Endpoint Genotyping, and GBTS services.

Here you can also:

• View an interactive workflow infographic detailing our user-friendly ordering, shipping, processing, and data retrieval.
• Access our online customer portal to register, submit projects. and review data.
• Request a quick quote

Seamless, reliable, and very competitive on price. We look forward to working with you.

For simplified collection and shipment of leaf samples for our premium DNA Extraction and Genotyping Lab Services.

Bis-propargyl-PEG12 has two alkyne groups at both ends of the linker. With the catalyzation of copper, the alkyne groups reacts with azide compounds to form stable triazole linkages. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-propargyl-PEG12 has two alkyne groups at both ends of the linker. With the catalyzation of copper, the alkyne groups reacts with azide compounds to form stable triazole linkages. The PEG spacer enhances the water-solubility of the compound. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.