Propargyl-PEG7-acid is a reagent with a propargyl group with a carboxylic acid. The carboxylic acid reacts with primary amines under the activation of EDC or HATU. The propargyl group can participate in azide-alkyne Click Chemistry reaction to form triazole linkage, copper is required as a catalyst. The PEG spacer increases the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG7-acid is a reagent with a propargyl group with a carboxylic acid. The carboxylic acid reacts with primary amines under the activation of EDC or HATU. The propargyl group can participate in azide-alkyne Click Chemistry reaction to form triazole linkage, copper is required as a catalyst. The PEG spacer increases the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Introduction

HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 150 mg plant and fungal tissue
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Fresh / frozen plant samples, dried plant / seed samples
Sample amount	Fresh / frozen plant samples: 50-150 mgDried plant / seed samples: 15-40 mg
Elution volume	≥40μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• Broad spectrum – suitable for extracting DNA from various plant samples
• Fast – several samples can be extracted in 30 minutes by silica technology
• Good repeatability – silica gel column purification technology can obtain ideal results every time

Kit Contents

Contents	D318702	D318703
Purification Times	50 Preps	250 Preps
Buffer PAL	60 ml	200 ml
Buffer GWP	60 ml	200 ml
Buffer DW1	30 ml	150 ml
Buffer GW2*	20 ml	50 ml
Buffer AE	20 ml	60 ml
HiPure DNA Mini Columns II	50	250
2 ml Collection Tubes	50	250

Storage and Stability

This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure HP Plant DNA Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.