Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Sulfo DBCO-PEG3-NHS ester is a water soluble, click chemistry reagent which can undergo copper free click chemistry Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate is a Succinimidyl ester/NHS-functionalized cyclooctyne derivative for addition of the cyclooctyne moiety into amine-containing compounds or biomolecules. The BCN group can react with azide-tagged biomolecules under copper free conditions.
(1R,8S,9s)-Bicyclo[6.1.0]non-4-yn-9-ylmethyl Succinimidyl Carbonate is a Succinimidyl ester/NHS-functionalized cyclooctyne derivative for addition of the cyclooctyne moiety into amine-containing compounds or biomolecules. The BCN group can react with azide-tagged biomolecules under copper free conditions.
The HighRanger 1 kb DNA Ladder is prepared to ensure quality and batch-to-batch consistency. This Ladder contains thirteen discrete fragments ranging from 300 bp to 10,000 bp with a higher intensity reference band at 5000 bp. This Ladder is ideal for quick sizing of PCR products and restriction digests.
Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).
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HighRanger 1kb DNA Ladder (Cat# 11900) – 100 loads
Ladder Properties:
• Thirteen discrete bands, ranging from 300 bp to 10000 bp
• Higher intensity band at 5000 bp for easy reference
| Fragment | Size (bp) | Mass (ng) |
| 1 | 10000 | 58 |
| 2 | 8000 | 53 |
| 3 | 6000 | 46 |
| 4 | 5000 | 67 |
| 5 | 4000 | 56 |
| 6 | 3000 | 47 |
| 7 | 2500 | 41 |
| 8 | 2000 | 34 |
| 9 | 1500 | 29 |
| 10 | 1000 | 18 |
| 11 | 700 | 13 |
| 12 | 500 | 23 |
| 13 | 300 | 14 |
Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.
Storage:
This kit is stable for 2 years after the date of shipment.
This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA from tissue, cell |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Tissues, cells, lymphocytes and other clinical sample |
| Sample amount | Cells grown in suspension:3~5 x 106Animal tissue: 10~20mgPlant tissue: ≤100mg |
The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt. The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase I. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water
Advantages
Kit Contents
| Contents | IVD3020 |
| Purification Times | 200 Preps |
| MagPure RNA Particles | 7 ml |
| DNase I | 4 x 600 µl |
| DNase Buffer | 80 ml |
| RTL Lysis Buffer | 150 ml |
| Buffer MCB* | 75 ml |
| Buffer MW1* | 110 ml |
| Buffer MW2* | 50 ml |
| RNase Free Water | 60 ml |
| Cat.No | Reagent | IVD3020-F-96 |
| DNase Buffer | 60 ml | |
| DNase I | 2 x 600 μl | |
| RTL Lysis Buffer | 80 ml | |
| Buffer MCB | 18 ml | |
| 96-Tip | 1 | |
| Sample plate (DW Plate) | 500μl Buffer MCB | 1 |
| Wash 1 Plate (DW Plate) | 700μl Buffer MW130μl MagPure RNA Partilces | 1 |
| DNase Plate | Empty | |
| Wash 2 Plate (DW Plate) | 700μl Buffer MW1 | 1 |
| Wash 3 Plate (DW Plate) | 900μl Buffer MW2 | 1 |
| Elution plate (DW Plate) | 80μl RNase Free Water | 1 |
| Cat.No | Reagent | IVD3020-TL-06 |
| Purification times | 96 Preps | |
| DNase I | 2 x 600 μl | |
| DNase Buffer | 60 ml | |
| RTL Lysis Buffer | 60 ml | |
| Buffer MCB | 40 ml | |
| 96-Tip | 12 PCS | |
| 2.0ml V-bottom plate | Row 1/7：500μl Buffer MCBRow 2/8：500μl Buffer MW1Row 3/9：emptyRow 4/10：30μl Magpure RNA Particles500μl Buffer MW2 Row 5/11：900μl Buffer MW2 Row 6/12：80μl RNase Free Water | 6 |
Storage and Stability
MagPure RNA Particles should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, MagPure RNA Particles up to 8 weeks) at roomtemperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid RNA extraction from tissue , cells, and other clinical samples. RNA can be used directly for RT-PCR, quantitative RT-PCR and so on.