Introduction

The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from <150 mg simple plant sample without chloroform
Applications	RT-PCR, qPCR, Northern hybridization, second generation sequencing, nucleic acid protection, in vitro translation
Purification method	Mini spin column
Purification technology	Silica technology, DNA filtration technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic crops
Sample amount	≤150 mg
Elution volume	≥30μl
Time per run	≤25 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

The Kit isolates total RNA from up to 150mg plant tissue. A short workflow enables RNA isolation with genomic DNA removal in less than 25 min. Samples are first lysed and homogenized. The lysate is passed through a DNA Mini column, ethanolis added to the flow-through, and the sample is applied to an RNA column. RNA binds to the membrane and contaminants are washed away. High-quality RNA is eluted in as little as 30µl water using the Kit.

Advantages

• Efficient removal of DNA – unique genomic DNA removal column without DNase treatment
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Fast – several samples can be extracted in 25 minutes by column method
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG
• Broad spectrum – various types of plant samples can be processed by diversity of operating procedures

Kit Contents

Contents	R415102	D415103
Purification Times	50 Preps	250 Preps
HiPure DNA Mini Column II	50	250
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	100	2 x 250
Buffer RLC	50 ml	200 ml
Buffer PRC1	50 ml	200 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability

The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Make sure that all buffers are at room temperature when used. During shipment, crystals or precipitation may form inthe Buffer RLC/PRC1. Dissolve by warming buffer to 37°C.

Purchase Guide

1. When dealing with woody or uncommon samples, R4150 is recommended first. R4150 contains two polysaccharide/polyphenol lysis buffer, which is the most universal product.

2. R4151 is recommended for handling common economic crop samples for the first time. Strong lysis solution can be used to process easy-extraction samples. The amount of corn or rice leaves samples can reach up to 300mg.

3. R4165 adopts CTAB/chloroform method, which can also handle a large number of difficult-to-extraction plants, but requires contact with chloroform substitutes, which is less safe than other kits. This kit uses DNase Ⅰ to remove DNA, which is also a good choice for extracting polysaccharide/polyphenol-rich plant samples.

4. R4014 is recommended for fruit/starch plant samples, which uses improved trizol pre-treatment, single column operation and is more economical.

Select the right purification kit to get impactful results：

Ⅰ. Purchase guide of Magen kits based on sample

Ⅱ. Cross purchase guide for Magen kits vs Other brands

For any customized product or OEM service, please contact us.

The Kit provides fast purification of high-quality RNA from plants, cell, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or precipitation with isopropanol or LiCl. RNA purified using the RaPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Introduction

Usages:

For differentiation of enterobacteriaceae.

Principle:

Peptone, beef extract powder and yeast extract powder provide nitrogen, vitamins, minerals; lactose, dextrose into fermentable sugars, which produce acid when measured by the phenol red indicator, acid yellow, basic red; thiosulfate sodium can be reduced to some bacteria to hydrogen sulfide, to produce a black iron sulfide and iron salts; sodium chloride to maintain osmotic equilibrium; agar as medium coagulant.

Formulation(per liter):

Peptone 20g

Beef extract powder 3g

Yeast extract powder 3g

Lactose 10g

Dextrose 1g

Sodium chloride 5g

Ferric ammonium citrate 0.5g

Sodium thiosulfate 0.5g

Agar 12g

Phenol red 0.05g

Final pH 7.4 ± 0.2

How to use:

1.Suspend 54.5g in 1L of distilled water , stirring heated to boiling ,autoclave at 121 for 15 minutes.

2.Diluted and treated samples.

Quality control:

Item	The name and number of strain	Growth	Colony Color
1	Escherichia coli ATCC25922	Good	A/A
2	Proteus CMCC (B) 49027	Good	K/A
3	Salmonella typhimurium CMCC (B) 50115	Good	K/A

“A”Acid , “K” Alkality

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

TD5B Large Capacity Low Speed Centrifuge

Features:

1. Brushless motor with simpler construction, more reliable performance, longer life and quietly running.
2. Automatically electric lid lock, super speed, over temperature protection and imbalance protection. The centrifuge body is made of high quality steel, safe and reliable.
3. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction, safe and reliable.
4. Microprocessor control, TD5B with digital display which indicate the speed, time, RCF in operation, speed raising and reducing quick, operate simply.
5.10 kinds of accelerating and decelerating speed for your choice.

TD5B Technical Parameter：

Max. Speed	5000rpm
Max. RCF	4730×g
Max. Capacity	4×800ml
Time Range	0~99min
RPM/RCF Convert	Yes
Noise (dB)	≤ 58
Temperature	Normal
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	/
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	685×500×385mm
Net Weight(Kg)	67KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for TD5B

Order No	Rotor	Max Speed(r/min)	Volume(ml)	Max RCF(×g)
5B-1	Swing Rotor	4000	4×800ml	3450
5B-2	Swing Rotor	4000	4×750ml	3530
5B-3	Swing Rotor	4000	4×500ml	3380
5B-4	Swing Rotor	4000	4×4×96(well)	2940
5B-5	Microplate rotor	4000	2×4×96(well)	3210
5B-6			2×3×48(well)	2300
5B-7	Swing rotor	5000	4×1×50ml	4730
			4×1×100ml	4730
		4000	4×2×100ml	3020
		4000	4×2×50ml	3200
		4000	4×4×15ml	3200
		4000	4×6×15ml	3200
5B-8	Swing rotor	4000	4×8×15ml	3200
		4000	4×12×5ml vacuum tube	2480
		4000	4×12×7ml vacuum tube	2760
		4000	4×12×10ml vacuum tube	2880
		4000	4×8×10/7/5ml vacuum tube	2790
		4000	4×6×10/7/5ml vacuum tube	2790
		4000	4×4×10/7/5ml vacuum tube	2790
5B-9	Swing rotor	4000	4×24×5ml vacuum tube	2480
		4000	4×31×5ml Khan tubes	2480
5B-10	Swing rotor	4000	4×24×7ml vacuum tube	3140
		4000	4×31×7ml Khan tubes	3140
		4000	4×30×7ml vacuum tube	3140
		4000	4×30×5ml vacuum tube	3140
		4000	4×40×7ml vacuum tube	3270
		4000	4×18×10ml vacuum tube	3140
5B-11	Swing rotor	4000	Dairy bottle	3830
5B-12	Fixed rotor	5000	6×15ml	2540
5B-13		5000	12×15ml	3080
5B-14		5000	24×15ml	3500
5B-15		5000	30×15ml	3830
5B-16		5000	4×50ml	2520
5B-17		5000	6×50ml	2850
5B-18		5000	12×50ml	3860
5B-19		4000	24×50ml	2970
5B-20		5000	4×100ml	2630
5B-21		5000	6×100ml	3130
5B-22		4000	12×100ml	2970

Adapter for 800ml and 500ml bucket

VolumeCup	5ml	7ml	10ml	15ml	50ml
800ml（Round Cup）	23pcs/cup	23	19	14	5
500ml（Round Cup）	18pcs/cup	18	14	9	3
500ml（Square Cup）	20pcs/cup	20	16	12	5

TD5B large capacity centrifuge max capacity can be reached to 4x800ml, can with different adapters for small tubes, 50ml, 15ml, 10ml, 7ml, 5ml, 2ml and so on.