Name of Product
Swab Detection Buffer
Catalog Number
MGSDB
Short Info
Extractionbuffer for swabs
Method/Platform
Buffer for lateral flow assay
Range/Assay Sensivity
Test Principle
The Milenia swab PCR-System allows the direct detection of obligate spoilers from a swab. Areas of poor Hygiene and hotspots of Biofilm Formation can be quickly identified and monitored.
Name of Product
Swab Detection Buffer
Catalog Number
MGSDB
Short Info
Extractionbuffer for swabs
Method/Platform
Buffer for lateral flow assay
Range/Assay Sensivity
Test Principle
The Milenia swab PCR-System allows the direct detection of obligate spoilers from a swab. Areas of poor Hygiene and hotspots of Biofilm Formation can be quickly identified and monitored.
Brief Instructions
Storage
Components
For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method.
MUG permits the rapid detection of Escherichia coli when the medium is observed for fluorescence using a UV light. MUG is hydrolyzed by the enzyme β-glucuronidase which is produced by E. coli to yield a fluorescent end product 4- methylumbelliferone. Trypticase provides the essential nutrients. Lactose is the fermentable carbohydrate. Sodium chloride maintains the osmotic equilibrium. The medium has a strong buffering system to control the pH in the presence of fermentative action. The bile salts inhibit gram-positive bacteria especially the Bacillus species and faecal Streptococci.
| Ingredients | /liter |
| Trypticase or tryptose | 20 g |
| Bile salts No. 3 | 1.5 g |
| Lactose | 5 g |
| K2HPO4 | 4 g |
| KH2PO4 | 1.5 g |
| NaCl | 5 g |
| 4-methylumbelliferyl-β-D-glucuronide (MUG) | 50mg |
| pH6.9±0.2 at 25°C | |
Weigh 37g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until
completely dissolved, sterilize at 115℃ for 20min, cool to room temperature and set aside.
The following quality control strains were inoculated and cultured at 44.5℃±0.5℃ for 24h. The results are as follows:
| Quality control strains | Growth |
| Escherichia coli ATCC25922 | Blue-white fluorescence is produced under 366nm UV light |
| Salmonella typhimurium ATCC14028 | No fluorescence under 366nm UV light |
| Enterococcus faecalis ATCC29212 | Clear, no fluorescence |
2-30℃，Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the determination of Escherichia coli in drinking water and its source water by multiple tube fermentation method. Principle and Interpretation MUG permits the rapid detec……
Description
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
Key Features
Storage and stability
Store Q-PAGE™ Precast Gels at 4°C for periods up to 12 months.
Do not freeze Q-PAGE™ Precast Gels Remove tape and comb before electrophoresis.
Keep Q-PAGE™ Precast Gels flat during storage.
Q-PAGE™ Bis-Tris Precast Gel is a high-performance and easy to use precast polyacrylamide gel for electrophoresis in Bis-Tris buffer system (MOPS or MES). The optimized gel formula allows Q-PAGE™ Bis-Tris Precast Gel to show improved resolution, accurate results, and an extended shelf-life over conventional Laemmli Tris-HCl gels.
Q-PAGE™ Bis-Tris Precast Gels are available in gradient (4 to 12%) and fixed (8% and 12%) concentrations of polyacrylamide in 12-and 15-well formats. Two available cassette sizes, Mini (10 x 8.3 cm) and Midi (10 x 10 cm), are compatible with most popular protein electrophoresis systems. Q-PAGE™ Mini (QP2XXX) Gels are suitable for Bio-Rad® and other systems. Q-PAGE™ Midi (QP3XXX) Gels are suitable for Invitrogen® XCell SureLock® Mini-Cell, Invitrogen® Mini Gel Tank, Hoefer SE260, and other systems.
20 bp DNA Ladder on 3.6% of agarose gel, 8% and 12% of acrylamide gel.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
• For sizing and quantification of double strand DNA fragments.
• Composed of eight bands as shown on right.
• Premixed with 6X DNA loading buffer for direct gel loading.
