Swab Detection Buffer

Overview

• RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
• Compatible with most DNA, Total RNA, microRNA and protein isolation methods
• Preservative is available in a single dose liquid format (ampule)
• Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
• Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups

Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.

Norgen’s Urine Preservative is available in 2 convenient formats:

1.  Urine Preservative Single Dose Ampules
With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.

2.  Urine Collection and Preservation Tubes
Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:

• 5 cc tubes, 1 – 5 mL of urine
• 15 cc tubes, 5 – 15 mL of urine
• 50 cc tubes, 5 – 45 mL of urine
• 120 cc cup, 100 – 120 mL of urine

Details

Supporting Data

Figure 1 / 2

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Select Urine Tested with the Norgen Urine Collection and Preservation Tubes

Human
Mouse
Lynx
Wolf
Urine collected from snow

Available Sizes and Formats 

1. Urine Preservative Single Dose Ampule – Urine inputs from 5 – 50 mL

Storage Conditions and Product Stability
All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.

Kit Components	Cat. 18118 (50 tubes)	Cat. 18122 (50 tubes)	Cat. 18113 (50 tubes)	Cat. 18126 (50 tubes)	Cat. 18129 (1 cup)
Urine Collection and Preservation Tubes (5 cc)	50	–	–	–	–
Urine Collection and Preservation Tubes (15 cc)	–	50	–	–	–
Urine Collection and Preservation Tubes (50 cc)	–	–	50	–	–
Urine Preservative Single Dose	–	–	–	50	–
Urine Collection and Preservation Cup (120 cc)	–	–	–	–	1
ID Labels	50	–	–	50	–
Product Insert	1	1	1	1	1

Overview

• Detection kits for Neisseria gonorrhoea
• Available in TaqMan format for analysis

Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is transmitted by sexual contact and usually causes infection in cells of the mucous membrane of the male urethra or the endocervix and urethra in females. During infection, polysaccharides are released from the bacteria that stimulate host cell production of tumour necrosis factors that cause an inflammatory response.

There is no vaccine against N. gonorrhoeae infection and antibiotic resistance is beginning to increase, therefore treatment includes a course of antibiotics that will be effective against resistant strains. Complications in males caused by the infection can result in prostatitis or orchitis if the bacteria spread. In females, invasion of the fallopian tubes or ovaries can result in salpingitis or ovaritis respectively, with any of these infections possibly resulting in sterility.

Neisseria gonorrhoea TaqMan PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

Neisseria gonorrhoea TaqMan PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s PCR Master Mix (#28007) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

Supporting Data

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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM30950 (100 preps)	Cat. TM30910 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
Neisseria gonorrhoea Primer & Probe Mix	280 μL	280 μL
Neisseria gonorrhoea Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1