t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Human Immunodeficiency Virus (HIV) is a retrovirus which destroys essential cells in the human immune system, such as CD4+ lymphocytes (a type of T cell), macrophages and dendritic cells. Infection with HIV therefore leads to an impaired immune system and increased susceptibility to opportunistic cancers and infections. Due to the mutating nature of HIV and damage to the T cells, the immune system is unable to fight off infections and disease. Without treatment, HIV can progress to Acquired Immunodeficiency Syndrome (AIDS). The rate of HIV progression to AIDS depends on a number of different factors including viral, host and environmental factors. However, with adherence to antiretroviral therapy (ART), many individuals with HIV will never progress to AIDS, having near normal life expectancy. Transmission of HIV is through direct contact with infected bodily fluids, either sexually; by the sharing of blood-contaminated needles or instruments; maternally through childbirth or breastfeeding; or via blood transfusion/organ transplant.
HIV TaqMan RT-PCR Kit, 100 reactions
HIV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM33750 (100 preps) | Cat. TM33710 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| HIV Primer & Probe Mix | 280 μL | 280 μL |
| HIV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
Specifications
| Features | Specifications |
| Main Functions | Isolation high pure total DNA from FFPE using high bind beads |
| Applications | PCR and viral DNA detection, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Paraffin embedded tissue samples |
| Sample amount | 1-6 slices of 10-20μm |
| Elution volume | ≥30μl |
| Time per run | ≤60 minutes |
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
| Contents | D632301D | D632302D |
| Purification Times | 48 Preps | 96 Preps |
| MagPure Particles N | 1.1 ml | 2.5 ml |
| RNase A | 10 mg | 20 mg |
| Proteinase K | 24 mg | 48 mg |
| Protease Dissolve Buffer | 3 ml | 6 ml |
| Buffer DPS | 60 ml | 100 ml |
| Buffer ATL | 15 ml | 30 ml |
| Buffer BST1 | 30 ml | 60 ml |
| Buffer BW1 | 13 ml | 44 ml |
| Elution Buffer | 15 ml | 30 ml |
Storage and Stability
Proteinase K, RNase A and MagPure Particles N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
This product is suitable for rapid extraction of DNA from FFPE sample. This kit uses two combination methods. High-salt Bind is conducive to remove pigments or polysaccharides from complex FFPE samples, so as to improve the purity of nucleic acid and avoid blocking aligent 2100. Alcohol mediated adsorption is conducive to improve the nucleic acid yield of high-yield samples.
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 60bp-20kbp from PCR products and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | PCR products, enzymatic reaction solution |
| Sample amount | 10-200μl |
| Recovery | ≥80% |
| Elution volume | ≥7μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 10µg |
The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
Kit Contents
| Contents | D212002 | D212003 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer DP | 60 ml | 120 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 15 ml | 30 ml |
| HiPure DNA Micro Columns | 100 | 250 |
| 2 ml Collection Tubes | 100 | 250 |
Storage and Stability
The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure PCR Pure Micro Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.
