t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
K-MALTA
SKU: 700004315
100 assays (50 of each) per kit
| Content: | 100 assays (50 of each) per kit |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | α-Amylase, β-Amylase |
| Assay Format: | Spectrophotometer |
| Detection Method: | Absorbance |
| Wavelength (nm): | 400 |
| Signal Response: | Increase |
| Limit of Detection: | 0.05 U/mL |
| Reaction Time (min): | ~ 20 min (Ceralpha Method), ~ 10 min (Betamyl-3 Method) |
| Application examples: | Cereal flours, malts, fermentation broths and other materials. |
| Method recognition: | “Ceralpha” Method: AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018). “Betamyl-3” Method: RACI Standard Method |
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
Other enzyme activity test kits available.
Advantages
The Malt Amylase test kit is suitable for the specific measurement and analysis of α-amylase and of β-amylase in malt flour.
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.
Kit Contents
| Contents | R401601 | R401602 |
| Purification Times | 1 x 96 | 4 x 96 |
| HiPure RNA Plate | 1 | 4 |
| 1.5ml Collection Plate | 1 | 4 |
| 0.5ml Collection Plate | 1 | 4 |
| RTL Lysis Buffer | 100 ml | 400 ml |
| RNA Binding Buffer* | 30 ml | 120 ml |
| Buffer RW1 | 100 ml | 400 ml |
| Buffer RW2* | 50 ml | 2 x 100 ml |
| RNase Free Water | 20 ml | 80 ml |
Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit provides a high throughput of high-quality RNA from 96 samples of cells, tissues, and yeast using silica-membrane spin column plate with a binding capacity of 100ug RNA. RNA purified using the HiPure Total RNA System is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Usages:
For cultivation of non-fastidious microorganisms, indole testing.
Principle:
Peptone provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride to maintain osmotic equilibrium.
Formulation(per liter):
Peptone 10g
Sodium chloride 5g
Final pH 7.0 ± 0.2
How to use:
1.Suspend 15g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, 121 autoclave for 15min.
2.Diluted and treated samples.
Quality control:
| Item | The name and number of strain | Growth | Colony Color |
| 1 | Escherichia coli ATCC25922 | Good | Turn Red |
| 2 | Klebsiella pneumoniae | Good | Color unchange |
500g
