t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
t-Boc-N-Amido-PEG4-propargyl is a propargyl linker with one side protected by Boc group. The propargyl group can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TD6 Table top laboratory centrifuge
TD6 Features:
1. Widely used in hospital, laboratory and blood bank.
2. Brushless DC motor for model TD6 which in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. The flexible axle driven system which drive the rotor directly, smooth in operation, low noise and small vibration.
4. Micro computer control system, digital display the RCF、time and speed. There are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. Electric lid lock, compact design, super speed and imbalance protection.
6. The centrifuge body is made of high-quality steel, safe and reliable.
TD6 Technical Parameters:
| Max speed | 6000rpm | Max RCF： | 4430xg |
| Max volume | 6x100ml | Noise： | ≤55dBA |
| Timer | 0～99min | Net weight： | 32KG |
| Dimension(HxDxW)： | 540×370×280mm | Power supply | AC 110/220V 50HZ/60hz |
| Speed accuracy | ±20rpm | Acc/Dec | 10 Kinds |
| Certifications | CE, ISO & Calibration report is available. | Warranty | 1 Year |
Matched Rotors for TD6:
| Order No. | Rotor | Max Speed (rpm) | Max Volume(ml) | Max. RCF(×g） |
| D6-1 | Angle Rotor | 6000 | 6×15/10/7/5ml | 3660 |
| D6-2 | Angle Rotor | 6000 | 12×15/10/7/5ml | 4430 |
| D6-3 | Angle Rotor | 6000 | 6×50ml | 4110 |
| D6-4 | Angle Rotor | 6000 | 4×50ml | 3620 |
| D6-5 | Angle Rotor | 6000 | 4×100ml | 3790 |
| D6-6 | Angle Rotor | 5000 | 24×15/10/7/5ml | 3500 |
| D6-7 | Angle Rotor | 4000 | 12×50ml | 2470 |
| D6-8 | Angle Rotor | 5000 | 6×100ml | 3130 |
| D6-9 | Swing Rotor | 5000 | 4x100ml | 4420 |
| D6-10 | Swing Rotor | 5000 | 4x50ml | 4420 |
| D6-11 | Swing Rotor | 5000 | 4x4x15ml | 2820 |
| D6-12 | Swing Rotor | 4000 | 4x6x10ml | 2580 |
| D6-13 | Swing Rotor | 4000 | 4x4x10ml | 2580 |
| D6-14 | Microplate | 4000 | 2x2x96well | 2020 |
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Specifications
| Features | Specifications |
| Main Functions | Isolation miRNA from 0.3-0.5ml serum or plasma using magnetic particles |
| Applications | RT-PCR, cDNA synthesis, second generation sequencing |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
| Sample type | Serum, plasma, acellular samples |
| Sample amount | 300μl |
This product is based on the purification method of high binding magnetic particles. The sample material is denatured in Lysis Buffer. The protein is then precipitated by Protein Precipitation Solution and pelleted by centrifugation. After adding magnetic particles and binding solution, RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally RNA was eluted by Elution Buffer.
Advantages
Kit Contents
| Contents | R662801 | R662802 | R662803 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles N | 1.7 ml | 3.5 ml | 17 ml |
| Buffer CFL | 6 ml | 12 ml | 60 ml |
| Buffer CPL | 1.8 ml | 3.5 ml | 20 ml |
| Buffer MGW1* | 30 ml | 60 ml | 250 ml |
| Buffer MW2* | 12 ml | 25 ml | 100 ml |
| RNase Free Water | 10 ml | 20 ml | 60 ml |
Storage and Stability
MagPure Particle N should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.
The kit offers the unique feature to isolate total RNA including small RNA and DNA from serum and plasma without the need to resort to the cumbersome phenol/chloroform extraction or a time consuming proteinase digest. RNA purified using the kit is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Mini Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. Samples can be fresh or frozen (provided that they have not been frozen and thawed more than once).
Specifications
| Features | Specifications |
| Main Functions | Isolation circulating DNA from 1ml plasma, serum, body fluids |
| Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Serum, plasma and other cell-free fluid samples |
| Sample amount | 1ml |
| Elution volume | ≥30μl |
| Time per run | ≤50 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
This product is based on silica gel purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption plate and filter column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10 Mm Tris,pH 8.0).
Kit Contents
| Contents | D318102 | D318103 |
| Purification Times | 50 Preps | 250 Preps |
| Buffer ACL | 50 ml | 250 ml |
| Buffer ACB* | 60 ml | 300 ml |
| Buffer DCW1* | 22 ml | 88 ml |
| Buffer DCW2* | 10 ml | 50 ml |
| Proteinase K | 120 mg | 540 mg |
| Protease Dissolve Buffer | 10 ml | 30 ml |
| Carrier RNA | 110 μg | 310 μg |
| Nuclease Free Water | 10 ml | 30 ml |
| HiPure CFDNA Mini Columns | 50 | 250 |
| 2 ml Collection Tubes | 100 | 500 |
Storage and Stability
Proteinase K and carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage(up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remainingkit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under these conditions.The entire kit can be stored at 2–8°C, but in this case buffers shouldbe redissolved before use. Make sure that all buffers are at room temperature when used.
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments,
