TCO-PEG12-DBCO is a long chain PEGreagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine under mild condition. DBCO is very reactive toward Azide through click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
TCO-PEG12-DBCO is a long chain PEGreagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine under mild condition. DBCO is very reactive toward Azide through click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
0.2 mL PCR Magnetic Separator 24-tube
Product Info
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Product Info
Permagen’s 0.2 mL PCR Strip Magnetic rack is designed for magnetic bead separations from Individual tubes, 8-strip, or 12-strip PCR tubes
Accommodates any common PCR strips or individual PCR tubes
Tubes are angled and beads will be pulled to back wall allowing easy aspiration and tip tracking down the front wall of the tubes without disturbing bead pellet
DBCO-PEG5-NHS Ester is a DBCO-containing PEG linker containing NHS ester that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG5 spacer increases the water solubility of compounds in aqueous media. DBCO is commonly used for copper-free Click Chemistry reaction. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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DBCO-PEG5-NHS Ester is a DBCO-containing PEG linker containing NHS ester that can react specifically and efficiently with primary amines (e.g. the side chain of lysine residues or aminosilane-coated surfaces) at neutral or slightly basic condition to form a covalent bond. PEG5 spacer increases the water solubility of compounds in aqueous media. DBCO is commonly used for copper-free Click Chemistry reaction. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
For the selective isolation and culture of Listeria monocytogenes.
Principle and Interpretation
Peptone provides the carbon and nitrogen sources necessary for growth; yeast extract powder and starch provide carbon and nitrogen sources, vitamins and growth factors; sodium chloride can maintain a balanced osmotic pressure; glucose provides a carbon source; Listeria hydrolyzes esculin and reacts with iron ions to form black 6,7-dihydroxycoumarin; mannitol is a fermentable sugar, and phenol red is a pH indicator; lithium chloride and other antibiotics can inhibit Gram-negative bacteria and most Gram-positive bacteria; agar is the coagulant of the culture medium.
Formulation
Ingredients
/liter
Peptone
23 g
Starch
1 g
NaCl
5 g
Columbia agar
13 g
Mannitol
10 g
Ferric ammonium citrate
0.5 g
Esculin (aesculin)
0.8 g
Dextrose (glucose)
0.5 g
Lithium chloride
15.0 g
Phenol red
0.08 g
pH7.2±0.2 at 25°C
Preparation
Weigh 68.9g of dry powder of this product, add 1L of distilled water or deionized water, stir, heat and boil until completely dissolved, divide into Erlenmeyer bottles, sterilize at 121℃ for 15min, cool to room temperature and set aside. Heat to dissolve agar before use, cool to 50℃, add 1 tube of supporting reagent (SR0140) per 100mL of basal culture medium, shake well and pour into sterilized culture dish.
Quality Control
The following quality control strains were inoculated and cultured at 35-37℃ for 24h. The results are as follows:
Quality control strains
Growth
Listeria monocytogenes ATCC19115
Gray-green colonies with a black depression in the center and black surrounding
Enterococcus faecalis ATCC29212
–
Escherichia coli ATCC25922
–
Storage and Shelf Life
2-30℃,Keep container tightly closed, avoid direct sunlight.
Use before expiry date on the label.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Document
Intended Use For the selective isolation and culture of Listeria monocytogenes. Principle and Interpretation Peptone provides the carbon and nitrogen sources necessary for growth; yeast ex……