TCO-PEG12-DBCO is a long chain PEGreagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine under mild condition. DBCO is very reactive toward Azide through click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TCO-PEG12-DBCO is a long chain PEGreagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine under mild condition. DBCO is very reactive toward Azide through click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Neisseria gonorrhoeae is a Gram-negative coccus of the Neisseria genus. N. gonorrhoeae is usually seen in pairs infecting human cells. It has a circular DNA genome of approximately 1Mbp encoding over 2000 genes. N. gonorrhoeae is transmitted by sexual contact and usually causes infection in cells of the mucous membrane of the male urethra or the endocervix and urethra in females. During infection, polysaccharides are released from the bacteria that stimulate host cell production of tumour necrosis factors that cause an inflammatory response.
There is no vaccine against N. gonorrhoeae infection and antibiotic resistance is beginning to increase, therefore treatment includes a course of antibiotics that will be effective against resistant strains. Complications in males caused by the infection can result in prostatitis or orchitis if the bacteria spread. In females, invasion of the fallopian tubes or ovaries can result in salpingitis or ovaritis respectively, with any of these infections possibly resulting in sterility.
Neisseria gonorrhoea TaqMan PCR Kit, 100 reactions
Neisseria gonorrhoea TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM30950 (100 preps) | Cat. TM30910 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Neisseria gonorrhoea Primer & Probe Mix | 280 μL | 280 μL |
| Neisseria gonorrhoea Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
【Material】The vial storage box is made of durable cardboard with waterproof coating and cardboard dividers；chemically resistant to alcohols and mild organic
【Temperature Range】These freezer boxes are stable from -196°C to 121°C
【Applications】The freezer box is suitable for iquid nitrogen freezing (vapor phase)
【Capacity】Size: Height of 2 inch, Holds 0.5ml, 1.5ml, 2.0ml tubes
【Excellent Cusomer Service】We are dedicated in providing the best products and services to customers. If you have any problem, please feel free to contact us. We will help you solve the problem as soon as possible.
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
NGS DNA Fragmentation & Library Prep Kit Workflow
The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The NGS DNA Fragmentation & Library Prep Kit does not generate sequencing bias as compared to library using mechanical sheared DNA as input. Sequence coverage is also consistent between enzymatic shearing and mechanical shearing. The library size is inversely correlated with the incubation time of step 1 at 20°C.
Three index types are available for the kit of the illumina platform:
Non-index (Cat.# 30026): Libraries do not have index.
Index (Cat.# 30028): Each of the index primers contains a unique index sequence of 6 bases. Library multiplexing for 48 samples is possible. Index information can be downloaded here.
Unique dual index (Cat.# 30030): Library multiplexing for 96 samples is possible. With the unique features of our 4-Base Difference Index System, the index sequence is 8-base long and each index has 4 bases different from others. Our unique dual index primers effectively identify sequencing errors such as index hopping, mis-assignment of reads, and de-multiplexing errors etc. The unique dual index primers set consists of 96 pre-mixed unique pairs of index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34028).
Kit features:
Library conversion efficiency: 100 ng, 300 ng and 500 ng of intact genomic DNA were used as input.
The library size is inversely correlated with the incubation time of step 1 at 20°C.
NGS data comparison: enzymatic shearing versus mechanical shearing
Enzymatic shearing
• DNA shearing and library prep: BioDynami NGS DNA Fragmentation & Library Prep Kit
Mechanical shearing
• DNA shearing: Covaris sonication
• Library prep: BioDynami NGS DNA Library Prep Kit.
The NGS DNA Fragmentation & Library Prep Kit (illumina and MGI Platforms) was developed for construction of high quality libraries for next generation sequencing. The kit uses intact genomic DNA (EDTA-free DNA or DNA resuspended in TE buffer) as input DNA without an additional DNA fragmentation step. Our technology provides a fast and simple workflow. DNA libraries can be generated around 2 hours with only 10 min hands-on time. Library multiplexing is possible.
83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand
Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
Copyright © 2024 A3P Scientific Co., Ltd. All rights reserved. Web by Mountain Studio
Privacy Policy | Terms of Use | Site Map