TCO-PEG4-DBCO is a heterobifunctional click chemistry reagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine at fast speed. DBCO is very reactive toward Azide through copper free click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TCO-PEG4-DBCO is a heterobifunctional click chemistry reagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine at fast speed. DBCO is very reactive toward Azide through copper free click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Description
ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.
Features
Application
Storage
-20°C for 24 months
| ComponentVolume ExcelRT™ Reverse Transcriptase (200 U/μl) 100 μl RNase Inhibitor (20 U/μl)100 μl 5X RT Buffer (DTT)500 μl dNTPs Mix (10 mM each)200 μl Oligo (dT)20 (50 μM)100 μl Random Hexamers (100 μM)100 μl DEPC-Treated H2O 1 ml x 2 |
Storage Buffer
Reverse Transcriptase: 20 mM Tris-HCl (pH 7.5), 200 mM NaCl, 0.1 mM EDTA, 1 mM DTT, stabilizer and 50% (v/v) glycerol
RNase Inhibitor: 40 mM HEPES-KOH (pH 7.5), 100 mM KCl, 8 mM DTT, 0.1 mM EDTA, stabilizer and 50% (v/v) glycerol
5X RT buffer (DTT)
250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2 and 50 mM DTT
Storage
-20°C for 24 months
ExcelRT™ Reverse Transcription Kit is a complete, efficient and convenient kit to synthesize high quality first strand cDNA. This kit contains ExcelRT™ Reverse Transcriptase, which is able to synthesize the first strand cDNA at 37~50°C. The ExcelRT™ Reverse Transcriptase is a recombinant Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase, which is designed to reduce RNase H activity and create better thermal stability. This kit also contains RNAok™ RNase Inhibitor, which is active against RNase A, RNase B, and RNase C. This product is supplied with oligo (dT)20 and random hexamers, which are used to synthesize cDNA from poly(A) tailed mRNA and total RNA, respectively.
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit. Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Human Cytomegalovirus (HCMV) or Human betaherpesvirus 5 (HHV-5) is a member of the Family Orthoherpesviridae. HCMV belongs to the betaherpesvirinae subfamily, while other herpesviruses fall into the subfamilies of Alphaherpesvirinae (including HSV 1 and 2 and varicella) or Gammaherpesvirinae (including Epstein-Barr virus). All herpes viruses share a characteristic ability to remain latent within the body over long periods of time. While CMV can be found in numerous body fluids including urine, saliva, breast milk, blood, tears, semen, and vaginal fluids, urine samples are generally used for congenital HCMV infection screening due to high viruria observed in infected infants.
CMV TaqMan PCR Kit, 100 reactions
CMV TaqMan PCR Probe/Primer Set and Controls, 100 reactions
For research use only and NOT intended for in vitro diagnostics.
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Storage Conditions and Product Stability
All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM36350 (100 preps) | Cat. TM36310 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| CMV Primer & Probe Mix | 280 μL | 280 μL |
| CMV Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
