TCO-PEG4-DBCO is a heterobifunctional click chemistry reagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine at fast speed. DBCO is very reactive toward Azide through copper free click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TCO-PEG4-DBCO is a heterobifunctional click chemistry reagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine at fast speed. DBCO is very reactive toward Azide through copper free click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Product Description
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
N-(Propargyl-PEG2)-N-bis(PEG3-t-butyl ester) is a 3-arm PEG reagent with a propargyl groups and two t-butyl ester groups. The propargyl group can react with azides via copper catalyzed Click Chemistry reaction to yield a stable triazole linkage. The t-butyl ester groups can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG2)-N-bis(PEG3-t-butyl ester) is a 3-arm PEG reagent with a propargyl groups and two t-butyl ester groups. The propargyl group can react with azides via copper catalyzed Click Chemistry reaction to yield a stable triazole linkage. The t-butyl ester groups can be deprotected under acidic conditions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
029080 Glycerin
Usage:Additive Reagent for Culture Media.
Specification:1ml*10vials
1ml*10vials
