TCO-PEG4-DBCO is a heterobifunctional click chemistry reagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine at fast speed. DBCO is very reactive toward Azide through copper free click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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TCO-PEG4-DBCO is a heterobifunctional click chemistry reagent containing a TCO and a DBCO moiety. TCO group specifically and efficiently reacts with terrahydrazine at fast speed. DBCO is very reactive toward Azide through copper free click chemistry, the PEG spacer increases the aqueous solubility of the reagent. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Animal Tissue RNA Purification Kit
Product Info
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Product Info
Overview
Extract high quality & quantity total RNA including miRNA
Isolate from a wide variety of animal tissues
No phenol step required – isolate all RNA in one fraction
Compatible with tissues stored in RNAlater® or Trizol®
Convenient & fast spin column format
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit is suitable for the isolation of total RNA from a range of animal tissues such as liver and spleen, as well as difficult fibrous tissues such as heart, muscle, intestine, etc. Briefly, the tissue of interest is first lysed using Buffer RL, followed by treatment with the provided Proteinase K which aids in the removal of the various proteins present in fiber-rich tissues including collagen, contractile proteins, and connective tissues. The purified RNA is of the highest quality and purity, with excellent RIN values and A260/A280, and is suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS, and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Maximum Amount of Starting Material Heart Kidney Liver Muscle Spleen
30 mg 15 mg 15 mg 30 mg 15 mg
Storage Conditions and Product Stability The DNAse I should be stored at -20°C upon arrival. The Proteinase K should be stored at -20°C upon arrival and after reconstitution. All other solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date shipment.
Propargyl-PEG3-NHS ester is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Propargyl-PEG3-NHS ester is a Click Chemistry reagent with a propargyl group and an NHS ester group. The propargyl group can react with biomolecules containing azide group via copper catalyzed Click Chemistry reaction. The NHS ester is an amine reactive group which can be used for derivatizing peptides, antibodies, amine coated surfaces etc. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
TCO-PEG24-DBCO is a monodisperse, long PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO easily reacts with azides through click chemistry, while the TCO group readily reacts with tetrazine-containing compounds.
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TCO-PEG24-DBCO is a monodisperse, long PEG linker featuring a trans-cyclooctene and a DBCO group. DBCO easily reacts with azides through click chemistry, while the TCO group readily reacts with tetrazine-containing compounds.