Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane

• Non-specific amplification is prevented by using this Taq DNA polymerase variant as the DNA polymerase is hotstart-formulated. The 10x reaction buffer supplied together with the DNA polymerase has been specifically designed for optimal PCR performance and DNA polymerase activity. This allows the use of this DNA polymerase in a wide range of PCR applications.For research use and further manufacturing. Designed and manufactured under ISO13485. Please contact us for bulk quantities and quotes.

Broad Amplification Range

Different-sized amplicons from 3 ng of a DNA plasmid were amplified. The use of Taq DNA polymerase resulted in clean and high yield of products, as analysed after PCR on a 0.8% agarose gel.

Faster Detection and Higher Sensitivity

A fragment (64 bp) of the human blood-coagulation factor IIa (F2) was amplified from 20 ng, 2 ng, 200 pg and 20 pg of a human genomic DNA extract. The same experiment was performed in parallel using the Taq DNA polymerase mix from another well-established and known supplier. PCR products were subsequently analysed on a 2.5% agarose gel.

Non-specific amplification is prevented by using this Taq DNA polymerase variant as the DNA polymerase is hotstart-formulated. The 10x reaction buffer supplied together with the DNA polymerase has been specifically designed for optimal PCR performance and DNA polymerase activity. This allows the use of this DNA polymerase in a wide range of PCR applications.

• Format: 96-well microtiter plate (12 test strips of 8 wells)
• Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
• Incubation Time: 45 Minutes
• Compatible for use with US EPA Method 546

Description

The Cylindrospermopsin plate kit is a competitive enzyme-labeled immunoassay. The Cylindrospermopsin sample extract and calibrators are pipetted into the test wells followed by the Cylindrospermopsin antibody into the test wells to initiate the reaction. During the 30 minutes incubation period, Cylin-drospermopsin from the sample and Cylindrospermopsin antigen compete for binding to the Cylindrosper-mopsin antibody. The Cylindrospermopsin antibody is captured on the walls of the test well. Following this 30-minute incubation, the contents of the wells are removed and the wells are washed to remove any unbound Cylindrospermopsin and free Cylindrospermopsin antibody. After wash, 1X HRP-conjugated Antibody#2 is added for 30 minutes incubation. The wells are washed afterwards, and a clear substrate is then added to the wells and any bound enzyme conjugate causes the conversion to a blue color. Following a 15-minute incubation, the reaction is stopped and the amount of color in each well is read. The color of the unknown samples is compared to the color of the calibrators and the Cylindrospermopsin concentration of the samples is derived.

Format:

• • 96-well microtiter plate (12 test strips of 8 wells)
• • Standards: 0 | 0.03 | 0.10 | 0.2| 2 ppb
• • Incubation Time: 45 Minutes
• Compatible for use with US EPA Method 546

EPA 10-Day drinking water Health Advisories for Cylindrospermopsin: 

Do not Drink – 0.7 μg/L for bottle fed infants and preschool children, pregnant and nursing woman, elderly immunocompromised and liver conditions.

Do not Drink – 3.0 μg/L for school age children to adults.

Do Not Use – 20 μg/L

EPA Draft Human Health Recreational Ambient Water Quality Criteria to protect human health: 8 μg/L.

Format: 96-well microtiter plate (12 test strips of 8 wells)
Standards: 0 | 0.03 | 0.10 | 0.2 | 2 ppb
Incubation Time: 45 Minutes
Compatible for use with US EPA Method 546

Description

Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.

The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.

Details of the target and priming specificity are included in the individual handbooks above.

Packaged, optimised and ready to use. Expect Better Data.

The Primerdesign™ genesig® Kit for Enterocytozoon bieneusi (E. bieneusi) genomes is designed for the in vitro quantification of E. bieneusi genomes. The kit is designed to have the broadest detection profile possible whilst remaining specific to the E. bieneusi genome. The primers and probe sequences in this kit have 100% homology with a broad range of E. bieneusi sequences based on a comprehensive bioinformatics analysis.