Tetra(3-methoxy-N-(PEG5-prop-2-ynyl)propanamide) Methane

DBCO-PEG13-DBCO is a long chain click chemistry reagent DBCO will react with azide-bearing compounds or biomolecules without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

DBCO-PEG13-DBCO is a long chain click chemistry reagent DBCO will react with azide-bearing compounds or biomolecules without copper catalyst. The hydrophilic PEG spacer arm can increase water solubility and membrane permability. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Overview

• Reduce endotoxin levels to 0.1 EU/µg DNA or less
• Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
• Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
• Fast and easy processing using a rapid spin-column format

These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.

Endotoxin Removal Kit (Mini)

This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.

Endotoxin Removal Kit (Midi)

This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

Endotoxin Removal Kit (Maxi)

This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.

About Endotoxins

Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.

Details

Supporting Data

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Kit Specifications
Maximum DNA Input	25 μg
Final Endotoxin Level	≤ 0.1 EU/µg DNA
Size of DNA Purified	Up to 13,000 bp
Maximum DNA Volume Input	100 μL
Average Recovery	> 90%
Time to Complete 10 Purifications	20 minutes

 
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 22700 (25 preps)	Cat. 52200 (10 preps)	Cat. 21900 (4 preps)
Buffer SK	15 mL	60 mL	60 mL
Wash Solution H	18 mL	18 mL	18 mL
Elution Buffer I	6 mL	12 mL	12 mL
Endotoxin Removal Solution	1.5 mL	1.5 mL	1.5 mL
Precipitation Solution	–	1.5 mL	1.5 mL
Spin Columns (assembled with Collection Tubes)	–	10	4
Spin Columns	25	–	–
Collection Tubes	25	–	–
Elution Tubes (1.7 mL)	25	–	–
Elution Tubes (15 mL)	–	10	–
Elution Tubes (50 mL)	–	–	4
Product Insert	1	1	1