TGM12 Table top capillary vessel Haematocrit centrifuge 

K-GAMINE

SKU: 700004290

50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)

Content:	50 assays (manual) / 500 assays (microplate) / 500 assays (auto-analyser)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	N-Acetyl-D-Glucosamine, D-Glucosamine, D-Glucosamine sulphate
Assay Format:	Spectrophotometer, Microplate, Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	4 to 80 µg of glucosamine per assay
Limit of Detection:	1.33 mg/L
Reaction Time (min):	~ 8 min
Application examples:	Food supplements, food products and beverages.
Method recognition:	Novel method

The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products. This kit can also be used to measure N-Acetyl-D-Glucosamine and D-Glucosamine sulphate.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

See our complete list of monosaccharide and disaccharide assay kits.

Advantages

• Novel product with simple format 
• All reagents stable for > 2 years after preparation 
• All enzymes supplied as stable suspensions 
• Very rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included 
• Suitable for manual, microplate and auto-analyser formats

The D-Glucosamine test kit is a high purity reagent for the measurement and analysis of D-glucosamine in neutraceutical/food products. This kit can also be used to measure N-Acetyl-D-Glucosamine and D-Glucosamine sulphate.

Name of Product

HybriDetect Cassette

Catalog Number

MGHC 1

Short Info

The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.

Please note, that you may have to pay country-specific taxes and duties.

Method/Platform

Lateral flow, sandwich or competetive

Range/Assay Sensivity

10 pmol/LFA – 1 fmol/LFA

Test Principle

Milenia HybriDetect Cassette is a ready-to-use, universal test unit, which is based on the lateral flow technology using gold nanoparticles. The test unit is designed to develop qualitative or semi-quantitative rapid test systems for several analytes such as proteins, antibodies, or gene amplicons. The user needs to develop an analyte-specific solution or reaction (PCR, RPA, LAMP, CRISPR/Cas, …), which contains a first detector (e.g. antibody, antigen, specific probe) labelled with FITC or FAM and a second one (e.g. antibody, primer) labelled with biotin.

The HybriDetect Cassette is the cassette version of our HybriDetect dipstick (MGHD 1). It is a simple and quick tool to develop your own rapid test and allows you and your clients a custumor friendly evaluation. Various molecules can be detected such as proteins, antibodies and genetic amplicons.

OverView

HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human  total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results  indicate that HL-dsDNase has minimal impact on RNA quality and quantity.

HL-dsDNase is an engineered version of dsDNase that is rapidly and completely inactivated by incubation for 5 minutes at 58°C with 1 mM DTT at pH 8.0 or above. Chemical inactivation in downstream compatible RT or PCR buffers allows milder heat inactivation and, in some cases, skipping heat inactivation altogether. This makes HL-dsDNase very useful for removal of DNA from RNA preparations since the enzyme may be inactivated using various strategies with reduced risk of auto-degradation of  RNA in the presence of magnesium, making HL-dsDNase an ideal enzyme when working with small volumens of RNA.

HL-dsDNase is also an excellent choice for removal of unwanted external DNA from samples prior to analysis of nucleic acids protected by biological membranes, e.g., bacteria, viruses, and sperm. Especially if using downstream analysis methods that might be affected by host cell DNA, as metagenomic sequencing and STR-profiling. The easy inactivation of HL-dsDNase makes it a fast and efficient alternative to methods as differential extraction, where sample material is often lost.

Recommended applications:

• Removal of genomic DNA from RNA preparations
• Improved removal of host-cell DNA from sperm cells prior to STR-profiling

Key advantages with HL-dsDNase:

• Double-strand DNA specific endonuclease
• Easily heat-inactivated by very moderate heat treatment
• High specific activity
• Useful for removal of DNA from RNA preps

Properties

HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human  total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results  indicate that HL-dsDNase has minimal impact on RNA quality and quantity.