[TK1000] ExcelTaq™ Klen-Taq DNA Polymerase, 5 U/μl, 500 U
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ExcelTaq™ Klen-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentrations of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. The ExcelTaq™ Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.
Detail
Description
ExcelTaq™ Klen-Taq DNA Polymerase is a specially blended enzyme mix containing KlenTaq-1 DNA polymerase (a 5’-exo-minus, N-terminal deletion of Taq DNA polymerase) and a small amount of a proofreading DNA polymerase. This unique blending helps to improve the fidelity, yield and processivity of the resultant PCR process. The Klen-Taq is also highly robust, showing high tolerance of varying concentrations of Mg2+; it is highly thermostable and has four times the fidelity compared to Taq DNA polymerase. The ExcelTaq™ Klen-Taq DNA Polymerase is ideal for DNA amplifications 0.5-5 kb in length on genomic DNA, and up to 10 kb on less complex templates.
Features
5’→3′ DNA polymerase activity
3’→5′ exonuclease activity (proofreading)
4× fidelity as compared to Taq DNA polymerase
Thermo-stable: up to 98°C during PCR denaturing step
Robust PCR performance, resistance to variance in PCR conditions
Storage
-20°C for 24 months
Other Products
P1001 HiPure Plasmid Mini Kit
Product Info
Document
Product Info
Introduction
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.
Compared to other domestic products, Magen was the first to solve the stability problem of the column. Many other brands have unstable extraction concentrations, and the longer the time, the more unstable the column becomes. However, in our test of Magen kit, the quality and yield of plasmid extraction still remain stable after 5 years’ storage. For different customers, our kits can be customized. For example, Classic type is suitable for customers with low copy or unclear plasmid types. The rapid type is suitable for customers with high copy plasmids. Compared to many other brands, the plasmid DNA extracted by Magen has a longer preservation time and more thoroughly RNA removal.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 35μg plasmid DNA from 1-5ml bacterial culture
Applications
Enzyme digestion, sequencing, PCR, cloning, etc.
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Conventional plasmid, plasmid less than 30KB
Sample amount
High copy plasmid: 1-5ml culture mediumLow copy number plasmid : 5-10ml culture medium
Yield
5-35µg
Elution volume
≥30μl
Time per run
Complete 1-24 samples in 30 minutes
Liquid carrying volume per column
800µl
Binding yield of column
35µg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
Fast – it takes only 1 minute to obtain supernatant by optimized solution (10 minutes for other brands)
High yield – up to 35µg plasmid can be binded in one column
Kit Contents
Contents
P100102
P100103
Purification Times
100 Preps
250 Preps
RNase A
5 mg
10 mg
Buffer P1
30 ml
80 ml
Buffer P2
30 ml
80 ml
Buffer P3
40 ml
100 ml
Buffer PW1
60 ml
140 ml
Buffer PW2*
20 ml
50 ml
Elution Buffer
15 ml
30 ml
HiPure DNA Mini Columns II
100
250
2 ml Collection Tubes
100
250
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
The HiPure Mini system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Plasmid Mini Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of tris buffer or water.
Introducing the SARS-CoV-2 Detection Kit – the perfect solution for quick and accurate COVID-19 infection testing. This revolutionary kit has been designed to detect the presence of SARS-CoV-2 in Nasopharyngeal swabs, providing you with a reliable and convenient way to determine whether individuals have been infected with the virus. With the ability to perform 48 tests per kit, our detection kit is the perfect solution for rapid and accurate testing.
Our detection kit is designed for maximum accuracy and reliability, with components that must be stored at -20°C to ensure optimal performance. Our convenient shipping process ensures that the kit arrives frozen and ready for use, so you can rest assured that you’ll have the results you need as quickly as possible.
The SARS-CoV-2 Detection Kit is the most reliable and accurate COVID-19 infection testing solution on the market. With its convenient packaging and easy-to-use design, our detection kit is the perfect solution for any medical facility looking to quickly and accurately test for the presence of SARS-CoV-2.
Keywords: Covid-19 Virus Detection Test Kit, Covid-19 Test Kit, Covid 19 Rapid Test Kit
Technical Parameters:
Parameter
Value
Product Name
SARS-CoV-2 DetectionORF1ab Gene NFO Kit
Manufacturer
Amp-future Company
Detection Limit
500-1000copies/µL
Sample Type
Nasopharyngeal Swab
Target Gene
ORF1ab Gene
Packaging
48 Tests/Box
Test Time
5-20mins
Kit Components
Primers, Probes, Enzymes, Buffers
Shipping
ice
Storage Temperature
-20°C
Applications:
Amp-future bio’s SARS-CoV-2 Detection Kit is a rapid test kit for the detection and identification of SARS-CoV-2 virus. This test kit is designed to detect the presence of SARS-CoV-2 virus in human sample materials, and it is suitable for detecting the virus in nasopharyngeal swab samples. It has a detection limit of 500-1000 copies per µL, and it is packaged with 48 tests per kit. The kit uses ORF1ab Genetarget gene for the detection of SARS-CoV-2 virus.
The Amp-future bio SARS-CoV-2 Detection Kit is an ideal solution for organizations and institutions looking for a rapid and reliable solution for the detection of SARS-CoV-2 virus. It can be used in healthcare settings, laboratories, and other research institutes to identify the presence of SARS-CoV-2 virus in human samples, and it can also be used in the diagnosis and treatment of COVID-19 patients. The kit is suitable for use in public health surveillance programs to monitor the spread of the virus, and it can also be used for the rapid diagnosis and monitoring of SARS-CoV-2 infections in high-risk areas.
The Amp-future bio SARS-CoV-2 Detection Kit is a reliable and cost-effective solution for the detection and identification of SARS-CoV-2 virus. It is manufactured in China, and it has been developed following the highest standards of quality assurance. This kit is an indispensable tool for organizations and institutions looking for a rapid and reliable solution for the detection of SARS-CoV-2 virus in human samples.
Support and Services:
At , our goal is to provide customers with the best SARS-CoV-2 Detection Kit available. To ensure customer satisfaction, we provide a variety of technical support and service options.
Customer Support: Our customer support representatives are available to answer any questions you may have about the SARS-CoV-2 Detection Kit, including its features, compatibility, and installation.
Online Documentation: Our online documentation provides detailed instructions on how to use the SARS-CoV-2 Detection Kit, as well as troubleshooting tips and other helpful information.
Warranty and Returns: We provide a limited warranty on our SARS-CoV-2 Detection Kit to protect our customers against manufacturer defects or malfunctions. If you experience any issues with your product, please contact us for assistance.
Software Updates: We regularly release software updates for the SARS-CoV-2 Detection Kit to ensure optimal performance. You can easily download and install the latest updates from our website.
If you have any questions or need further assistance, please contact us at and one of our representatives will be happy to help.
FAQ:
Q1: What is SARS-CoV-2 Detection Kit?
A1: SARS-CoV-2 Detection Kit is a product produced by Amp-future bio, which is originated from China. It is used for detecting the presence of SARS-CoV-2 virus in a sample.
Q2: What is the principle of SARS-CoV-2 Detection Kit?
A2: SARS-CoV-2 Detection Kit utilizes the PCR (polymerase chain reaction) technology to amplify small amounts of the virus’s genetic material, allowing it to be detected with high accuracy.
Q3: What type of sample can be tested with SARS-CoV-2 Detection Kit?
A3: SARS-CoV-2 Detection Kit is suitable for testing samples such as nasal swab, nasopharyngeal swab, sputum, saliva, throat swab and bronchoalveolar lavage fluid.
Document
Introducing the SARS-CoV-2 Detection Kit – the perfect solution for quick and accurate COVID-19 infection testing. This revolutionary kit has been designed to detect the presence of SARS-CoV-2 in Nasopharyngeal swabs, providing you with a reliable and convenient way to determine whether individuals have been infected with the virus. With the ability to perform 48 tests per kit, our detection kit is the perfect solution for rapid and accurate testing.
Our detection kit is designed for maximum accuracy and reliability, with components that must be stored at -20°C to ensure optimal performance. Our convenient shipping process ensures that the kit arrives frozen and ready for use, so you can rest assured that you’ll have the results you need as quickly as possible.
The SARS-CoV-2 Detection Kit is the most reliable and accurate COVID-19 infection testing solution on the market. With its convenient packaging and easy-to-use design, our detection kit is the perfect solution for any medical facility looking to quickly and accurately test for the presence of SARS-CoV-2.
HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results indicate that HL-dsDNase has minimal impact on RNA quality and quantity.
HL-dsDNase is an engineered version of dsDNase that is rapidly and completely inactivated by incubation for 5 minutes at 58°C with 1 mM DTT at pH 8.0 or above. Chemical inactivation in downstream compatible RT or PCR buffers allows milder heat inactivation and, in some cases, skipping heat inactivation altogether. This makes HL-dsDNase very useful for removal of DNA from RNA preparations since the enzyme may be inactivated using various strategies with reduced risk of auto-degradation of RNA in the presence of magnesium, making HL-dsDNase an ideal enzyme when working with small volumens of RNA.
HL-dsDNase is also an excellent choice for removal of unwanted external DNA from samples prior to analysis of nucleic acids protected by biological membranes, e.g., bacteria, viruses, and sperm. Especially if using downstream analysis methods that might be affected by host cell DNA, as metagenomic sequencing and STR-profiling. The easy inactivation of HL-dsDNase makes it a fast and efficient alternative to methods as differential extraction, where sample material is often lost.
Easily heat-inactivated by very moderate heat treatment
High specific activity
Useful for removal of DNA from RNA preps
Properties
Document
HL-dsDNase is especially developed to remove contaminating genomic DNA from RNA preparations. Figure 1 shows that HL-dsDNase can reduce 50 ng of human gDNA to levels non-detectable by qPCR. In figure 2, a human total RNA sample was treated with HL-dsDNase and analysed on the Bio-Rad Experion™ System. The results indicate that HL-dsDNase has minimal impact on RNA quality and quantity.
