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Total RNA Purification Kits

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Detail

Overview

  • Extract high quality & quantity total RNA including miRNA
  • No phenol step required; isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
  • Available in a variety of formats to suit your needs
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)

This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.

Isolate RNA after Purifying EVs and Exosomes

Ultracentrifugation, Exoquick, Filtration

Cat. #NameElution VolumePlasma/SerumUrineCell-Culture Media
55000Plasma/Serum RNA Purification Mini Kit10 – 25 µL50 µL – 1 mL250 µL – 1 mL5 – 10 mL
35300Total RNA Purification Micro Kit20 – 50 µL1 – 4 mL2 – 10 mL10 – 20 mL
17200Total RNA Purification Kit50 – 100 µL4 – 10 mL11 – 30 mL20 – 35 mL

Details

Supporting Data

Figure 1 / 13

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Figure 1. High Quality of Isolated RNA with Complete Size Range.
Figure 1. High Quality of Isolated RNA with Complete Size Range.
Figure 2. Amplification of Both Large and Small RNAs in the Same Extraction.
Figure 3. High Quality of RNA from a Diverse Range of Inputs.
Figure 4. Great Isolation Sensitivity.
Figure 5. Linear and Sensitive Isolation of Both Large and Small RNA.
Figure 6. Effective and Consistent Detection of miRNA from Plasma.
Figure 7. Recovering Diverse miRNA Species from Plasma with Better Consistency.
Figure 8. Better Diversity of miRNA Detected from Plasma.
Figure 9. Better Diversity of miRNA Detected from HeLa Cells.
Figure 10. Total RNA Purification 96-Well Kit
Figure 11. Total RNA Purification 96-Well Kit
Figure 12. Total RNA Purification 96-Well Kit
Figure 13. Total RNA Purification 96-Well Kit

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Click for expanded view

Kit Specifications
Maximum Binding CapacityUp to 50 μg RNA
Maximum Loading Volume650 μL
Size of RNA PurifiedAll sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Animal Cells3 x 106 cells
Animal Tissues10 mg (for most tissues*)
Blood100 μL
Plasma/Serum200 μL
Bacteria1 x 109 cells
Yeast1 x 108 cells
Fungi50 mg
Plant Tissues50 mg
Time to Complete 10 Purifications20 minutes
Average Yield
HeLa Cells (1 x 106 cells)15 μg
E. coli (1 x 109 cells)50 μg

* for isolating total RNA from larger amounts of tissue, please use Norgen’s Animal Tissue RNA Purification Kit (Cat# 25700)

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.

ComponentCat. 17200 (50 preps)Cat. 37500 (100 preps)Cat. 17250 (250 preps)Cat. 17270 (500 preps)Cat. 24300 (192 preps)Cat. 24370 (576 preps)Cat. 24350 (192 preps)Cat. 24380 (576 preps)
Buffer RL40 mL2 x 40 mL175 mL350 mL2 x 40 mL350 mL2 x 40 mL350 mL
Wash Solution A38 mL2 x 38 mL148 mL1 x 148 mL
1 x 74 mL
2 x 38 mL1 x 74 mL
1 x 148 mL
2 x 38 mL1 x 74 mL
1 x 148 mL
Elution Solution A6 mL2 x 6 mL30 mL60 mL2 x 20 mL60 mL2 x 20 mL60 mL
Mini Spin Columns50100250500
96-Well Isolation Plate26
96-Well Isolation Plate (Deep Well)26
Adhesive Tape412412
Collection Tubes50100250500
96-Well Collection Plate26
96-Well Collection Plate (Deep Well)26
Elution Tubes (1.7 mL)50100250500
96-Well Elution Plate26
96-Well Elution Plate (Deep Well)26
Product Insert11111111

Other Products

Exosomal RNA Isolation Kit (for the extraction of RNA from EVs that have been isolated using IZON’s qEV columns)

Overview

  • Extract high quality & quantity total RNA including miRNA
  • No phenol step required; isolate all RNA in one fraction
  • Bind & elute all RNA irrespective of size or GC content, without bias
  • Very sensitive & linear down to a few cells without the need for carrier RNA
  • Isolate from a wide variety of specimens
  • Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
  • Available in a variety of formats to suit your needs
  • Purification is based on spin column chromatography that uses Norgen’s resin separation matrix

Norgen’s Exosomal RNA Isolation Kit (for the extraction of RNA from EV’s that have been isolated using IZON’s qEV columns) constitutes an all-in-one system for the isolation of exosomal RNA from exosomes previously isolated using IZON’s qEV columns or concentrated using IZON’s EV Concentration kit. This kit allows for the isolation of RNA from intact extracellular vesicles (EVs) where the purification is based on spin column chromatography that employs Norgen’s proprietary resin.

The kit is designed to isolate all sizes of extracellular vesicle RNA, including microRNA. The kit provides a clear advantage over other available kits in that it does not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 μL to 100 μL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PR, reverse transcription PCR, Nothern blotting, RNase protection and primer extension, and expression array assays.

Details

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature (15–25°C). This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

ComponentCat. 68910 (50 Preps)
Lysis Buffer A2 x 30 mL
Lysis Additive B7 mL
Wash Solution A38 mL
Elution Solution A6 mL
Mini Spin Columns50
Collection Tubes50
Elution Tubes (1.7 mL)50
Product Insert1

Carbendazim ELISA Kit

Description

Carbendazim is a fungicide used for the treatment and control of fungal diseases. Carbendazim continues to be used, frequently in combination with other fungicides.

This kit is based on ELISA technology, which is fast, easy, accurate and sensitive compared with common instrumental analysis and only needs 1.5 hours in one operation, it can considerably minimize operation error and work intensity.

IVD3141 HiPure Stool DNA Kit

Introduction

With the development of molecular biology, stool, a new non-invasive sample, has been widely used in the research of animal molecular genetics, population ecology, behavioral ecology and some intestinal disease diagnosis. Stool samples includes gut microbial DNA, food residue sample DNA, and alimentary tract exfoliated cell DNA.

The primary problem encountered when using stool sample for molecular biology research is the low content of exfoliated cells in the digestive tract and a certain degree of degradation of genetic material in stool. Another issue in molecular scatology research based on PCR is the presence of a large number of inhibitors in stool that can affect Taq enzyme activity, leading to downstream detection inactivation. These inhibitors include polysaccharides, plant polysaccharides, bile acids, bile salts, bile pigments, digestive juices, mucus, etc. Therefore, selecting appropriate extraction methods to obtain high-quality DNA is the key to successful downstream detection of stool DNA.

At present, the pretreatment methods used in the laboratory, such as phenol/chloroform extraction, cetyltrimethyl bromide (CTAB) lysis, and guanidine isothiocyanate lysis, lack universality in different species, and the success rate of extracting DNA for PCR amplification is also very low. The HiPure Stool DNA Kit provided by Magen Company has opened up a new approach for DNA extraction from stool samples with good universality, high cost-effectiveness, high yield and purification. The reagent kit adopts a unique solution system and inhibitory factor adsorbent, which can efficiently remove various impurities in stool samples. The purified DNA can be directly used for PCR, quantitative PCR and other applications.

This product allows rapid and reliable isolation of high-quality genomic DNA from various stool samples. Up to 100 mg soil samples can be processed in 60 minute. The system combines the reversible nucleic acid binding properties of HiPure matrix with the speed and versatility of spin column technology to eliminate PCR inhibiting compounds such as humic acid from soil samples. Purified DNA is suitable for PCR, restriction digestion, and next-generation sequencing. There are no organic extractions thus reducing plastic waste and hands-on time to allow multiple samples to be processed in parallel.

Details

Specifications

FeaturesSpecifications
Main FunctionsIsolation total DNA from 50-100mg stool samples
ApplicationsPCR, Southern Blot, enzyme digestion and NGS, etc.
Purification methodMini spin column
Purification technologySilica technology
Process methodManual (centrifugation or vacuum)
Sample typeStool
Sample amount50-100mg
Yield3-15μg
Elution volume≥30μl
Time per run≤60 minutes
Liquid carrying volume per column750μl
Binding yield of column100μg

Principle

Stool sample is homogenized and then treated in a specially formulated buffer containing detergent to lyse bacteria, yeast, and fungal samples. Humic acid, proteins, polysaccharides, and other contaminants are removed using our proprietary Absorber Solution. Binding conditions are then adjusted and the sample is applied to a DNA Mini Column. Two rapid wash steps remove trace contaminants and pure DNA is eluted in low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

Advantages

  • High purity – unique adsorbent can completely remove inhibitory factors
  • High concentration – maximum extraction of total DNA from stool samples
  • High recovery – DNA can be recovered at the level of PG
  • Good repeatability – silica technology can obtain ideal results every time

Kit Contents

ContentsIVD3141
Purification Times50 Preps
HiPure DNA Mini Columns II50
2ml Collection Tubes50
2ml Bead Tubes50
Proteinase K24 mg
Protease Dissolve Buffer1.8 ml
Buffer SPL40 ml
Buffer PCI40 ml
Buffer AL20 ml
Buffer GW122 ml
Buffer GW220 ml
Buffer AE15 ml

Storage and Stability

Proteinase K and Buffer PCI should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2–8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.