The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.
Detail
Description
The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.
Features
5’→3’ DNA polymerase activity
No detectable 3’→5′ exonuclease (proofreading) activity
Generates PCR products with 3′-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
Includes tracking dye for direct loading after PCR
Applications
Routine PCR
Colony PCR
High throughput PCR
Amplification of DNA fragments up to 8 kb
Generation of PCR products for TA cloning
DNA labeling
Storage
4°C for 6 months -20°C for 24 months
Other Products
α-Amylase Assay Kit (Ceralpha Method)
Product Info
Document
Product Info
K-CERA
SKU: 700004273
100 assays per kit
Content:
100 assays per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
α-Amylase
Assay Format:
Spectrophotometer, Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
400
Signal Response:
Increase
Limit of Detection:
0.003 U/g
Reproducibility (%):
~ 3%
Total Assay Time:
~ 30 min
Application examples:
Cereal flours, fermentation broths and other materials.
Method recognition:
AACC Method 22-02.01, AOAC Method 2002.01, ICC Standard No. 303, RACI Standard Method and CCFRA (Flour Testing Working Group Method 0018)
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
All reagents stable for > 2 years after preparation
Very specific
Simple format
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Validation of Methods
Document
The Ceralpha Method: α-Amylase test kit is suitable for the specific measurement and analysis of α-amylase in cereal grains and fermentation broths (fungal and bacterial).
A device that uses the principle of high-frequency oscillation to generate heat through the friction of molecules in PVC hoses, thereby melting the PVC hose and achieving the sealing effect. This device is also referred to as a pipe sealing machine in some places.
Cell Culture Media Exosome Purification and RNA Isolation Kits
Product Info
Document
Product Info
Overview
Purification and enrichment of intact cell culture media exosomes for functional studies
Isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias.
Versatile cell culture media input volumes
No phenol extractions, Proteinase K treatment, nor carrier RNA required
No time-consuming ultracentrifugation, filtration nor special syringes required
No precipitation reagents nor overnight incubation required
Pure exosomes are purified and are free from any other RNA-binding proteins
Purification is based on spin column chromatography that uses Norgen’s proprietary resin
The purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
Norgen’s Cell Culture Media Exosome Purification and RNA Isolation Kits constitute an all-in-one system for the purification of exosomes and the subsequent isolation of exosomal RNA from different cell culture media sample volumes. The purification is based on spin column chromatography that employs Norgen’s proprietary resin. The kit is designed to isolate all sizes of RNA, including microRNA, irrespective of size or GC content, without bias. These kits provide a clear advantage over other available kits in that they do not require any special instrumentation, protein precipitation reagents, extension tubes, phenol/chloroform or protease treatments. Moreover, the kits allow the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The RNA isolated from the purified exosomes is free from any protein-bound circulating RNA and is of the highest integrity. The purified RNA can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.
Cell Culture Media Exosome Purification and RNA Isolation Mini Kit
For sample input volumes ranging from 5 mL to 10 mL.
Cell Culture Media Exosome Purification and RNA Isolation Midi Kit
For sample input volumes ranging from 10 mL to 20 mL.
Cell Culture Media Exosome Purification and RNA Isolation Maxi Kit
For sample input volumes ranging from 20 mL to 35 mL.
All sizes, including miRNA and small RNA (<200 nt)
Elution Volume
50-100 µL
Time to Complete 10 Purifications
35 to 40 minutes
Average Yields*
Variable depending on specimen
* Please check page 4 of the product insert for Average Yields and Common RNA Quantification Methods
Storage Conditions and Product Stability All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years from the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.
