The ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis.
Detail
Description
The ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis.
Features
5’→3’ DNA polymerase activity
No detectable 3’→5′ exonuclease (proofreading) activity
Generates PCR products with 3′-dA overhangs
High yield PCR
High reproducibility
Reduced pipetting errors
For direct loading into electrophoresis analysis
DNA bands can be visualized directly under UV or 470 nm blue light illumination
Applications
Routine PCR
Colony PCR
High throughput PCR
Amplification of DNA fragments up to 8 kb
Generation of PCR products for TA cloning
DNA labeling
Storage
Protected from light and avoid multiple freeze/thaw cycles 4°C for 6 months -20°C for 24 months
Other Products
D3113 HiPure Tissue&Blood DNA Midi Kit
Product Info
Document
Product Info
Introduction
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern Blotting. Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.
Details
Specifications
Features
Specifications
Main Functions
Isolation total DNA from 2ml blood and 200mg tissue using Midi column
Applications
PCR, southern bolt and virus detection, etc
Purification method
Midi spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Tissue, cell, blood, saliva, swab, blood spot, semen and other clinical samples
Sample amount
0.2-2 ml
Elution volume
≥300μl
Time per run
≤80 minutes
Liquid carrying volume per column
4ml
Binding yield of column
1mg
Principles
This product is based on silica column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
Fast – without separation of leukocytes, organic extraction or ethanol precipitation
Simple – all nucleic acids can be obtained by direct digestion
Wide applicability – handle a variety of liquid samples
Kit Contents
Contents
D311302
D311303
Purification Times
20
100
HiPure gDNA Midi Columns
20
100
15ml Collection Tubes
40
200
Buffer ATL
50 ml
250 ml
Buffer AL
50 ml
250 ml
Buffer GW1*
22 ml
110 ml
Buffer GW2*
12 ml
50 ml
RNase A
20 mg
90 mg
Proteinase K
100 mg
440 mg
Protease Dissolve Buffer
10 ml
30 ml
Buffer AE
20 ml
120 ml
Storage and Stability
Proteinase K, RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
Stool Nucleic Acid Collection and Preservation Devices Dx
Product Info
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Product Info
Overview
CE-IVDR marked in accordance with the European Commission Regulation (EU) No. 2017/746.
Ideal for use in in vitro workflows
No need to immediately process samples
Nucleic acid preservation at room temperature over 2 years for DNA and 7 days for RNA
Ship stool samples at room temperature safely
Compatible with most DNA/RNA isolation methods
Norgen’s Stool Nucleic Acid Collection and Preservation Devices Dx are designed for the collection and preservation of nucleic acids from fresh stool specimens. The Stool Nucleic Acid Collection and Preservation Devices Dx – 50 contains 50 individual Stool Nucleic Acid Collection and Preservation Devices Dx. Each Stool Nucleic Acid Collection and Preservation Device Dx consists of Norgen’s Stool Nucleic Acid Collection and Preservation Tube Dx containing Norgen’s Stool Preservative in a liquid format. The user simply collects stool into the tubes (fill up to the line indicated on the tube) and mixes gently until the stool is well submerged under the preservative. The Stool Nucleic Acid Collection and Preservation Tube Dx is subsequently sent to the laboratory for DNA and/or RNA isolation and analysis. The stool DNA in preserved samples is stable for more than 2 years at room temperature, and the stool RNA in preserved samples is stable for 7 days at room temperature. DNA can be isolated from the preserved stool samples using Norgen’s Stool DNA Isolation Kit Dx (Cat. Dx27600) and RNA can be isolated from the preserved stool samples using Norgen’s Stool Total RNA Purification Kit Dx (Cat. Dx49500). These tubes are ideal for collecting and preserving DNA and RNA samples for in vitro diagnostic use for medical purposes.
NOTE: This product is not available for sale in the United States.
Extract high quality & quantity total RNA including miRNA
No phenol step required; isolate all RNA in one fraction
Genomic DNA Removal Column for efficient elimination of gDNA
Bind & elute all RNA irrespective of size or GC content, without bias
Efficiently extract small RNA irrespective of GC content
Very sensitive & linear down to a few cells without the need for carrier RNA
Convenient & fast spin column format
Isolate from a wide variety of specimens
Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step.
Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.
The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
HeLa Cells (1 x 106 cells)E. coli (1 x 109 cells)15 µg50 µg
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
