Description
The ExcelTaq™ 5X Fluorescent PCR Master Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as a master mix for virtually all PCR applications. This product is supplied as a 5X concentrated mixture containing all the essential ingredients for PCR with the exception of templates and primers. In addition, the mixture contains electrophoresis tracking dye (Bromophenol blue) and a safer fluorescent DNA staining dye, which enables the user to track the electrophoresis process in real time as well as eliminates the need for the staining process. The resultant PCR reaction mixture is sufficiently dense enough to be loaded directly into a TAE or TBE buffered gel for electrophoresis.
Features
- 5’→3’ DNA polymerase activity
- No detectable 3’→5′ exonuclease (proofreading) activity
- Generates PCR products with 3′-dA overhangs
- High yield PCR
- High reproducibility
- Reduced pipetting errors
- For direct loading into electrophoresis analysis
- DNA bands can be visualized directly under UV or 470 nm blue light illumination
Applications
- Routine PCR
- Colony PCR
- High throughput PCR
- Amplification of DNA fragments up to 8 kb
- Generation of PCR products for TA cloning
- DNA labeling
Storage
Protected from light and avoid multiple freeze/thaw cycles
4°C for 6 months
-20°C for 24 months
