Description
The ExcelTaq™ Blood Direct DNA Polymerase is designed for amplifying targeted DNA directly from whole blood, eliminating the need for a lengthy DNA isolation process. ExcelTaq™ Blood Direct DNA Polymerase is highly tolerant in the presence of PCR interfering/ inhibiting substances in blood, such as IgG, hemoglobin, and lactoferrin. ExcelTaq™ Blood Direct DNA Polymerase is compatible with most anticoagulants, such as citrate, EDTA, and heparin (Fig. 1). The ExcelTaq™ Blood Direct DNA Polymerase includes a pair of Positive Control Primers (CCR5) that are compatible with primate blood samples.
Features
- 5’→3′ DNA polymerase activity
- No detectable 3’→5′ exonuclease (proofreading) activity
- Generates PCR products with 3′-dA overhangs
- Compatible with most anticoagulants
Applications
- Direct amplification of DNA from blood samples
- High throughput screening without DNA purification
- Suitable for multiplex PCR
Storage
-20°C for 24 months
4℃ for 6 months
