[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 RXN

The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is a ready-to-use reagent with all the essential components for quantitative real-time PCR (qPCR) except primers and templates. The master mix contains a highly stable hot-start Taq polymerase in an optimized buffer with dsDNA specific SYBR green fluorescent dye. Consequently, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) features high stability during storage, even at 37°C for weeks. In addition to high sensitivity and signal intensity, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) performs a low background/ high specificity qPCR results, as well as a better compatibility with fast PCR program.

With inert smart blue contrast dye, the ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is ready-to-use and greatly reduces pipetting errors, while largely improving the reproducibility of the process. The ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX) is also compatible with ROX reference dye if ROX is recommended by the manufacturer of the qPCR system. This master mix allows sensitive, precise amplification, real-time tracking of the amplification process, and simultaneous quantification for targeted DNA molecules.

Description

Features

High Stability
Fast Hot Start
High Sensitivity
Low Background
Suitable for Fast Program
Smart Blue Contrast Dye

Storage

Protect from light.
Aliquot to avoid multiple freeze-thaw cycles.
-20°C for 12 months

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West Nile Virus (WNV) TaqMan RT-PCR Detection Kits

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Overview

Detection kits for WNV
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis

West Nile Virus (WNV) belongs to the RNA virus family of Flaviviridae. It is known to spread primarily through arthropod vectors such as mosquitoes. The virus infects mainly birds but is also reported to infect humans as well as pets such as cats and dogs. In humans, the majority of the infections caused by WNV are asymptomatic. However, some individuals (the majority of the confirmed cases) could enter a second, febrile stage with flu-like symptoms – commonly known as West Nile Fever. In a more serious stage, the disease becomes neuroinvasive, causing meningitis or encephalitis. Such severe conditions could lead to mortality. As the symptoms of WNV are very similar to other common diseases but can be fatal at a severe stage, it is important to distinguish the virus early in the diagnosis, particularly at the molecular level.

WNV TaqMan RT-PCR Kit, 100 reactions

Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents

WNV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
Nuclease-free water
Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

Figure 1 / 3

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Component	Cat. TM44250 (100 preps)	Cat. TM44210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
WNV Primer & Probe Mix	280 μL	280 μL
WNV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1

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A3P Scientific

A3P Scientific Head Office

83, On-nut 88/2 Prawet Sub-district, Prawet District, Bangkok, 10250, Thailand

Tel : 081-875-1869 , 02-328-7179
Email : [email protected]

Content:	100 assays per kit
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8^oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	Histamine
Assay Format:	Spectrophotometer
Detection Method:	Absorbance
Wavelength (nm):	492
Signal Response:	Increase
Linear Range:	0.5 to 12 μg of histamine per assay
Limit of Detection:	~ 0.09 μg of histamine per test or ~ 0.75 mg/Kg (ppm) of histamine in a fish sample
Limit of Quantification:	~ 0.5 mg of histamine per test or ~ 4.2 mg/Kg (ppm) of histamine in a fish sample
Reproducibility (%):	~ 3%
Reaction Time (min):	~ 20 min
Application examples:	Fish samples in various forms (e.g. fresh, canned, frozen, etc).

Max. Speed	8000rpm
Max. RCF	14900×g
Max. Capacity	8×2000ml(16*500ml blood bag）
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 65
Temperature	-20~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/380V 50HZ/60HZ
Dimensions (L x W x Hmm)	940×890×1000mm /
Net Weight(Kg)	600KG
Certificates	CE,ISO & Calibration report are available

Order No.	Rotor No.	Max Speed (rpm)	Max Volume(ml)	Max. RCF（×g）
8M-1	Swing Rotor	4000	8 x 2000ml	5716
8M-2	Swing Rotor	4000	6×2400ml	5330
8M-3	Angle Rotor	8000	6×1000ml	14900
8M-4	Swing Rotor	4200	2×6×1000ml	5680

[TQ1200] ExcelTaq™ 2X Fast Q-PCR Master Mix (SYBR, no ROX), 200 RXN

Detail

Other Products

Histamine Assay Kit

West Nile Virus (WNV) TaqMan RT-PCR Detection Kits

Overview

Details

Supporting Data

DL8M Large Capacity Refrigerated Centrifuge

A3P Scientific

A3P Scientific Head Office

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