Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate

The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition,  all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of  hands-on time. Library multiplexing up to 12 samples is possible.

NGS DNA Fragmentation & Library Prep Kit Workflow

The incorporation of DNA fragmentation in the kit makes it possible to directly use intact genomic DNA as input DNA without the need of mechanical DNA shearing or enzymatic DNA fragmentation. The protocol is optimized to generate libraries from 200 bp to 500 bp in size. The library size is inversely correlated with the incubation time of step 1 at 20°C.

Kit features:

• • • Fast: 1-hour library construction from intact genomic DNA to NGS library
    • • Total time: 1 hr
      • Hands-on time: 5 min
    • Intact genomic DNA can be used directly as input; DNA fragmentation is not required.
    • Works with both EDTA-free DNA samples and DNA samples in TE buffer
    • Simple workflow
    • • Three steps
      • Only one beads purification step
    • Guaranteed quality: high library conversion efficiency based on our chemistry

Library conversion efficiency for NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform). 100 ng, 300 ng and 500 ng of DNA were used as input.

NGS DNA Fragmentation & Library Prep Kit (Ion Torrent Platform): Library size distribution with different incubation time.

The NGS DNA Fragmentation & Library Prep Kit (ion torrent platform) was developed for construction of high quality libraries for next generation sequencing for ion torrent platform. The kit uses intact genomic DNA (both EDTA-free DNA and DNA in TE buffer are compatible) as input DNA without an additional DNA fragmentation step. Our NGS kit has a fast and simple workflow with only three step. In addition, all steps can be performed in one tube. The DNA libraries can be generated within one hour with less than 10 minutes of hands-on time. Library multiplexing up to 12 samples is possible.

Propargyl-PEG8-acid has an alkyne group and an acid group. The acid group can derivatize amine-containing molecules in the presence of activators such as EDC or HATU. The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG8-acid has an alkyne group and an acid group. The acid group can derivatize amine-containing molecules in the presence of activators such as EDC or HATU. The alkyne group can react with azides via copper catalyzed Click Chemistry reactions. The PEG units help increase the solubility of the molecule in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.

N-(Amino-PEG4)-N-bis(PEG4-propargyl) HCl salt is a trifunctional linker with two terminal alkynes and a primary amine. The terminal alkynes can be linked to azides using copper click chemistry while the primary amine is reactive towards carboxylic acids, NHS esters, ketones and aldehydes to form a variety of stable bonds.