Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Detail
Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The terminal carboxylic acid can react with primary amino groups in the presence of activators (e.g. EDC, or HATU) to form a stable amide bond. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Other Products
Urine Collection and Preservation Devices
Product Info
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Product Info
Overview
RNA/microRNA/DNA/Proteins are preserved for more than 2 years at room temperature in Norgen’s Urine Preservative
Compatible with most DNA, Total RNA, microRNA and protein isolation methods
Preservative is available in a single dose liquid format (ampule)
Preservative is also available in a dried format in tubes – Urine Collection and Preservation Tubes
Urine Collection and Preservation Devices are available in 4 convenient sizes: 5 cc tubes, 15 cc tubes, 50 cc tubes, and 120 cc cups
Norgen’s Urine Preservative is designed for the rapid preservation of DNA, RNA, microRNA and proteins from fresh urine samples. In addition, the Urine Preservative eliminates the need to immediately process or freeze samples and allows the samples to be shipped to centralized testing facilities at ambient temperatures. The components of the Urine Preservative allow samples to be stored for over 2 years at room temperature with no detected degradation of urine DNA, RNA or proteins.
Norgen’s Urine Preservative is available in 2 convenient formats:
1. Urine Preservative Single Dose Ampules With this product Norgen’s Urine Preservative is supplied in a liquid format in single dose ampules. The user simply collects 5 – 50 mL of urine into a urine collection container and adds the contents of the Urine Preservative Single Dose (Cat# 18126). The urine and preservative are then mixed, and the urine nucleic acids and proteins are preserved at room temperature.
2. Urine Collection and Preservation Tubes Norgen’s Urine Preservative is also available in a dried format in Norgen’s Urine Collection and Preservation Tubes. The user simply collects urine into the tubes and mixes gently until the orange preservative pellet in the tube has dissolved. Norgen’s Urine Collection and Preservation Tubes are available in 3 convenient sizes:
Select Urine Tested with the Norgen Urine Collection and Preservation Tubes
Human Mouse Lynx Wolf Urine collected from snow
Available Sizes and Formats
Urine Collection and Preservation Tubes (15 cc) – Urine inputs from 5 – 15 mL
Storage Conditions and Product Stability All tubes should be kept tightly sealed and stored at room temperature (15 – 25°C) for up to 2 years without any reduction in performance.
Features of the resDNASEQ CHO Residual DNA Quantitation kit include:
Simpler and Rapid
Only three steps will be need for Sample Preparation and All components of the Sample Preparation Kit can be stored at room temperature.
Only one Reagent for qPCR;
Only 1.5 hours will be needed for the whole test.
Accurate
Perfect amplification curve, good amplification efficiency and good precision.
Highly sensitive quantitation using proven TaqMan™ real-time qPCR technology.
Limit of Detection (LOD): 0.01 fg/μL; Limit of Quantification (LOQ): 0.3 fg/μL.
The recovery rate of different concentration samples in the linear range is between 70% and 130%.
Kit Performance
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 0.3fg/μL, 3fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99992, and amplification efficiency was 100.370%.
Fig 3. Five concentration samples of 0.1 fg/μL, 0.3 fg/μL, 0.5 fg/μL, 1 fg/μL and 3 fg/μL were detected, and 10 multiple wells were detected for each concentration. The CV of concentrati on values of samples with 0.3 fg/μL and above concentrations were less than 30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
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Note: Price not include shipment & duty, contact us to get full quote. The resDNASEQ CHO Residual DNA Quantitation kit is designed for the quantification of residual DNA from CHO, in cell lines which are used for production of biopharmaceutical products. The Ducky Bio residual DNA CHO Assay, based on proven real-time qPCR technology, makes testing of residual DNA from the Chinese hamster ovary (CHO) cell line rapid, specifc. The PCR-based assay is sensitive and specific for DNA from the CHO cell line and not subject to detection of human or environmental DNA that might be introduced during sample handling. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng CHO DNA per therapeutic dose).
The contamination of pathogenic microorganisms and their toxins in food and water is a serious issue for human health and safety. For instance, enterotoxins (SEs) produced by Staphylococcus aureus (S. aureus) are heat-stable, meaning pathological activity remains even after exposure to sterilization techniques and digestive proteases. Among the SEs, staphylococcal enterotoxin A (SEA) and B (SEB) are confirmed toxins which cause enteritis and food poisoning. Symptoms include nausea, vomiting, diarrhea, which in severe cases, may lead to fatalities in children and the elderly. These SEs, known as superantigens, non-specifically activate Tcells, leading to proliferation which ultimately results in T-cell elimination. This activation directly and indirectly induces a massive release of inflammatory cytokines.
In addition to acute poisoning, researchers reported that these toxins may play roles in the pathogenesis of autoimmune diseases. More specifically, intestinal dysbiosis (enteromicrobial imbalance) was found in patients with rheumatoid arthritis (RA) and which may overwhelm the host immune defense functions by chronic exposure to excess amounts of these pathogens (7, 8). In animal models, SEs synergistically play a role in the pathogenesis of autoimmune-related diseases, such as atopic dermatitis, food allergies, colitis, arthritis, and systemic lupus erythematosus.
Attogene is offering this highly robust test to detect SEB by lateral flow in diverse liquid sample types.
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Rapid dipstick test for detecting Staphylococcus Enterotoxin B (SEB) in liquid samples Detects 10 ppb and greater Staphylococcus Enterotoxin B (SEB) in the sample