Tri-(Propargyl-PEG2-ethoxymethyl)-methane-amido-PEG3-carboxylate

Overview

• Extract high quality & quantity total RNA including miRNA
• No phenol step required – isolate all RNA in one fraction
• Rapid processing in under 40 minutes
• Isolate total RNA from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

Norgen’s Total RNA Purification Maxi Kit provides a rapid method for the isolation and purification of total RNA from cultured animal cells, tissue samples, blood, bacteria, yeast, fungi, plants, and viruses. The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). The RNA is preferentially purified from other cellular components such as proteins, without the use of phenol or chloroform. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real-time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Norgen’s Purification Technology

Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The RNA is preferentially purified from other cellular components such as proteins without the use of phenol or chloroform. The process involves first lysing the cells or tissue of interest with the provided Buffer RL (please see the flow chart on page 4). Ethanol is then added to the lysate, and the solution is loaded onto a maxi spin column. Norgen’s resin binds RNA in a manner that depends on ionic concentrations. Thus only the RNA will bind to the column, while the contaminating proteins will be removed in the flowthrough or retained on the top of the resin. The bound RNA is then washed with the provided Wash Solution A in order to remove any remaining impurities, and the purified total RNA is eluted with the Elution Solution A. The purified RNA is of the highest integrity, and can be used in a number of downstream applications.

Details

Supporting Data

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Kit Specifications
Maximum Column Binding Capacity	1.5 mg
Maximum Column Loading Volume	20 mL
Size of RNA Purified	All sizes, including small RNA (< 200 nt)
Maximum Amount of Starting Material
Liver Tissue	100 mg
Heart Tissue	50 mg
Kidney Tissue	100 mg
Brain Tissue	250 mg
Lung Tissue	100 mg
Whole Blood	2 – 10 mL*
Bacteria	2.5 x 10 10 cells
Yeast	2 x 108 cells
Fungi	1 g
Plant Tissues	1 g
Time to Complete 4 Purifications	40 minutes
Average Yield: HeLa Cells (5 x 107 cells) E. coli (2.5 x 1010 cells)	~750 μg ~1.5 mg

*For isolating total RNA from purified leukocytes

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.

Component	Cat. 26800 (8 preps)
Buffer RL	2 x 40 mL
Wash Solution A	4 x 38 mL
Elution Solution A	3 x 20 mL
RNA Maxi Spin Columns (with collection tubes)	8
Elution Tubes (50 mL)	8
Product Insert	1

Opentrons OT-2 8-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for medium- to high-throughput workflows.

Opentrons OT-2 8-Channel Electronic Pipettes automate liquid handling and pipetting for most daily wetlab applications. Best for medium- to high-throughput workflows.

Description

The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors. 

Features

• Ideal for PCR amplification and cDNA synthesis
• Premixed solution
• Nuclease and ribonuclease free

Applications 

• PCR amplification of DNA fragments
• DNA fill-in reaction
• DNA sequencing
• Reverse transcription
• One-step RT-PCR

Storage

-20°C for 36 months

The SMOChem™ Deoxynucleotide (dNTP) Mix is an aqueous solution that contains an equimolar solution of ultrapure dATP, dCTP, dGTP and dTTP, each at a concentration of 10 mM at pH 8.5. The dNTP Mix is designed for many different molecular biology applications that involved in DNA synthesis or labeling, such as PCR, real-time PCR, DNA sequencing, reverse transcription, primer extension, and etc. The dNTP Mix is free of exo-deoxyribonuclease and endo-deoxyribonuclease as well as ribonuclease activity. The dNTP Mix offers the possibility to reduce the number of pipetting steps and the risk of reaction set up errors.