Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.
Real-time fluorescent DNA amplification made possible with a TwistAmp® exo kit. Recommended for users who want to combine TwistDx’s RPA amplification technology with the use of TwistDx’s proprietary fluorescent TwistAmp® exo probe in a homogenous format. The user need only supply primers, probe, and template. See manual for more information. Click to order oligonucleotides.
Fig 1. Only three steps will be need for Sample Preparation and only 20 minitutes will be taken for Sample Preparation.
Fig 2. Seven concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, 30fg/μL, 300fg/μL, 3pg/μL, 30pg/μL, 300pg/μL were detected. CV of each concentration was < 30%, Regression coefficient associated with standard solutions was 0.99975, and amplification efficiency was 100.068%.
Fig 3. Four concentration samples of 5fg/μL, 10fg/μL, 20fg/μL, and 30fg/μL were detected, and 10 multiple holes were detected for each concentration. The detection values of 5fg/μL and above were CV ＜30%.
Fig 4. DNA recovery can be determined by including samples spiked with known DNA amounts which are prepared from the corresponding DNA standards. Typically, the range for this value varies from 70% to 130%.
Fig 5. Only one Reagent for qPCR MIX.
Note: Price not include shipment & duty, contact us to get full quote.
The resDNASEQ E.coli Residual DNA Quantitation kit is designed for the quantification of residual DNA from E. coli, in cell lines which are used for production of biopharmaceutical products. The resDNASEQ E.coli Residual DNA Quantitation kit use TaqManTM quantitative PCR to perform rapid, specifc quantitation of femtogram levels of residual host-cell or plasmid DNA. The kit was developed to meet the sensitivity requirements defined by WHO (10 ng E. coli DNA per therapeutic dose).
Usages:
For the growth of pathogenic and non-pathogenic microorganisms.
Principle:
Polypeptone provide carbon and nitrogen sources, vitamins and growth factors; Neisseria starch can promote the growth characteristics and enhanced hemolytic streptococci; sodium chloride to maintain osmotic balance; agar as culture medium coagulant.
Formulation (per liter):
Peptone: 10g
Beef extract powder :10g
Sodium chloride: 5g
Agar :15g
Final pH 7.4 ± 0.2
How to use:
1. Suspend 40g of the product, adding 1 L of distilled or deionized water, ,heated to boiling stirring until completely dissolved, dispensing into flask, 121 autoclaved 15min.
2.After the medium is cooled to about 50 , sterile procedures, basal medium was added per 100mL defibrinated sheep or rabbit blood 5-10mL, shake pour plate; also dispensing sterile tubes set into the slope. Spare.
3. take the bacteria to be tested fresh pure cultures inoculated crossed or coated on the plate, set the temperature of the incubator training requirements were cultured for a predetermined time.
4. Observe the results.
Quality control:
The following were inoculated after 36 ± 1 for 24h , the results are as follows:
bacteria name bacteria No. growth status colony characteristics
Escherichia coli ATCC25922 not good hemolysis
Staphylococcus aureus, ATCC6538 good β-hemolytic
Beta-hemolytic streptococcus CMCC (B) 32210 good β-hemolytic
Streptococcus pneumoniae CMCC (B) 31001 good α-hemolytic
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
500g
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
Specifications
| Features | Specifications |
| Main Functions | Selectively recover DNA from PCR products and enzymatic reaction solution (Replace Beckmen or agencourt AmPure XP) |
| Applications | NGS, DNA library |
| Purification technology | Magnetic beads technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | DNA products, restriction endonuclease systems, or other enzymatic reaction solutions |
| Sample amount | Appropriate |
| Recovery | 80% |
| Operation time | ≤50 minutes |
This product is based on the purification method of high binding magnetic particles. PCR amplicons mix with MagPure A3, 100bp and larger DNA binds to magnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure and finally DNA was eluted by Elution Buffer or Water.
Advantages
Kit Contents
| Contents | BP-5 | BP-50 | BP-500 |
| MagPure A4 XP | 5 ml | 50 ml | 500 ml |
Storage and Stability
MagPure A4 XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 4 weeks) at room temperature (15-25°C) does not affect its performance. — Shake the reagent well before use. It should appear homogenous and consistent in color.
DO NOT FREEZE.
MagPure A4 XP utilizes Magen’s solid-phase paramagnetic bead technology for high-throughput purification of PCR amplicons. AmPure utilizes an optimized buffer to selectively bind PCR amplicons 100bp and larger to paramagnetic beads. Excess primes, nucleotides, salts and enzymes can be removed using a simple washing procedure. The resulting purified PCR product is essentially free of contaminants.
