Bis-propargyl-PEG13 comprises two propargyl groups which can form triazole linkages with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The hydrophilic PEG units help improve the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
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Bis-propargyl-PEG13 comprises two propargyl groups which can form triazole linkages with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The hydrophilic PEG units help improve the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Details
Specifications
Features
Specifications
Main Functions
Isolation up to 30μg endotoxin-free plasmid DNA from 1-5ml bacterial culture using 96-well bind plate
Applications
Cell transfection, animal injection, etc.
Purification method
96 well plate
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Low copy plasmid vector
Sample amount
1-5ml LB（x96）
Yield
30μg
Elution volume
≥75μl
Time per run
≤60 minutes
Liquid carrying volume per column
800μl
Binding yield of column
70μg
Principle
The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).
Advantages
1. High throughput: processing 96 samples at once; 2. High yield: The purified plasmid yield can reach up to 30μg, which is greater than the yield of the magnetic bead method kit; 3. Detoxification: endotoxin content <0.1EU/μg; 4. Fast: The entire extraction can be completed in 60 minutes without the need for time-consuming alcohol precipitation;
Kit Contents
Contents
P115701
P115702
P115703
Purification Times
1 x 96 Preps
4 x 96 Preps
20 x 96 Preps
RNase A
10 mg
20 mg
100 mg
Buffer P1
30 ml
120 ml
550 ml
Buffer P2
30 ml
120 ml
550 ml
Buffer LEN3
15 ml
60 ml
300 ml
Buffer LN4
80 ml
270 ml
2 x 700 ml
Buffer LN5
40 ml
220 ml
1100 ml
Buffer PW1
40 ml
220 ml
1100 ml
Buffer PW2
25 ml
100 ml
2 x 200 ml
Elution Buffer
30 ml
60 ml
250 ml
Lysate Clear Plate
1
4
20
HiPure DNA Plate
1
4
20
1.6ml Collection Plate
2
8
40
0.5ml Collection Plate
1
4
20
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve. After addition of RNase A，Buffer P1 is stable for 6 months when stored at 2-8°C.
The HiPure Minipreps system provides a fast, simple, and cost-effective plasmid DNA miniprep method for routine molecular biology laboratory applications. HiPure Miniprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Permagen’s direct replacement magnet plate for either PN: A32782 & A001322. Comes with spring cushion base and same strength magnets to meet current protocol requirements. 49% Cost savings*
For faster separations you can upgrade to our SKU# MSP500R which comes with all of the same features, just stronger magnets.
Compatible with any magnetic beads & all protocols requiring a 96S super magnet plate
Permagen’s direct replacement magnet plate for either PN: A32782 & A001322. Comes with spring cushion base and same strength magnets to meet current protocol requirements. 49% Cost savings*
