Tyrosinase Inhibitor Screening Kit (Colorimetric) provides a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of tyrosinase inhibitors. Tyrosinase catalyzes the oxidation of tyrosine, producing a chromophore that can be detected at OD = 510 nm. In the presence of kojic Acid, a reversible inhibitor of tyrosinase, the rate of oxidation of the substrate is decreased.
Tyrosinase or polyphenol oxidase is an oxidoreductase that participates in the biosynthesis of melanin, a ubiquitous biological pigment found in hair, eyes, skin, etc. Inhibition of tyrosinase has been a long-time target in the skin health research, cosmetics and agricultural industries because of its role in browning reactions in skin pigmentation and during fruit harvesting and handling.
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Clostridium difficile TaqMan PCR Detection Kits
Product Info
Document
Product Info
Overview
Detection kits for Clostridium difficile
CE-IVD marked version available for in vitro diagnostic use
Available in TaqMan format for analysis
Clostridium difficile is rod-shaped, gram positive bacterium. It is the main causal agent of antibiotic-associated diarrhea and pseudomembranous colitis. The colonization of intestines by C. difficile is usually associated with the elimination of natural intestinal flora as a result of antibiotic application and is frequently reported in health care centers. While C. difficile infection could be severe and life-threatening, particularly among the elderly, many patients are asymptomatic making diagnosis challenging during outbreaks. The tradition method of detecting C. difficile infection is by cytotoxicity test of the toxin produced by the bacterium, but such protocols usually require extensive time before conclusion can be made.
Storage Conditions and Product Stability All kit components can be stored for 2 years after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
Cryptosporidium is a parasite found in water that causes an infection in mammals termed cryptosporidiosis. It is one of the most common water-borne diseases and is found world-wide. It affects the intestines of mammals and typically causes an acute short-term infection. The most common symptom is self-limiting diarrhea in healthy individuals, however in immunocompromised individuals the symptoms are particularly severe and often fatal. There is no specific treatment for cryptosporidiosis other than fluid rehydration and management of any pain. Therefore early detection of Cryptosporidium in water is the foremost action to prevent the infection.
Cryptosporidium TaqMan RT-PCR Kit, 24 reactions
Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
Specific Primer and Probe mix for the pathogen/virus/viroid of interest
Primer and Probe mix
Positive and negative control to confirm the integrity of the kit reagents
The Cryptosporidium TaqMan PCR Kit Dx is shipped on dry ice. The components of the kit should be frozen upon arrival. If one or more of the components is not frozen when the kit is received, or if any of the components have been compromised during shipment, please contact Norgen Biotek for assistance. All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 3 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
Details
Specifications
Features
Specifications
Main Functions
Recover DNA fragments >100bp from agarose gel(<0.3g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
The Kit method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments (>100bp) and larger to paramagnetic beads. Excess primers, nucleotides, salts, and enzymes can be removed using a simple washing procedure.
Advantages
High recovery efficiency – up to 80% DNA recovery
General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
Kit Contents
Contents
D500101
D500102
D500103
Purification Times
50 Preps
500 Preps
5000 Preps
Buffer GDP
30 ml
250 ml
3 x 900 ml
MagPure Particles
1.6 ml
15 ml
3 x 50 ml
Buffer DW1
20 ml
180 ml
2 x 800 ml
Elution Buffer(10mmTris, pH8.5)
10 ml
60 ml
500 ml
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
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A highly efficient, easily automated agarose gel or PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration. The Kit can be easily used in manual and automated 96 or 384-well formats.
