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Ultrapure Nuclease-Free Water

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Detail

Overview

  • ASTM Type I Water
  • Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
  • Nuclease-free, protease-free, endotoxin-free
  • Suitable for molecular biology applications (qPCR, NGS etc.)
  • Suitable for cell culture applications
  • Produced in an ISO certified GMP laboratory
  • Water system is routinely monitored for compliance with QC standards

The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.

Product Specifications
pHn/a (highly pure water does not contain enough ions for an exact pH determination.)
Recommended StorageRoom Temperature
GradeUltrapure (ASTM Type I)
Biological ActivityDNase-, RNase-, Protease-, Endotoxin- Free
TOCs< 50 ppb
Resistivity> 18 MΩ-cm
TreatmentNot DEPC-Treated
Quantity100 mL, 500 mL, 1000 mL
Purification MethodsActivated charcoal, polyphosphate, reverse osmosis, electro-deionization, ion exchange, UV, ultrafiltration
Shipping ConditionsRoom Temperature

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.

Other Products

HAM005 Peptone Bacteriological

Introduction

Usages:
Peptone obtained by enzymatic digestion of selected animal tissues, widely used in the preparation of microbiological culture media.

Technical specification:
Total nitrogen(TN)—————————≥14.5%
Amino nitrogen(AN) ————————≥2.0%
Moisture—————————————≤5.0%
Ash———————————————≤5.0%
Clˉ——————————————–≤3.0%
PH(2% solution)——————————5-7

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.

Specifications: 500g/bottle; 10kg /bag

Conveniently Test RNA Isothermal PCR Kit At Home Reagent Buffer

Product Description

Conveniently Test RNA At Home With At Home Test Kit – Reagents Buffers

Product Detail

Kit Storage and term of Validity

Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.

Term of Validity: 14 months

Isothermal nucleic acid Principle Summary

This kit is based on a rapid nucleic acid amplification technology at room temperature and constant temperature:at room temperature and constant temperature(generally 39℃~42℃),reverse transcriptaseuses specific primers and template RNA tosynthesize cDNA strands,and binds the auxiliary protein and single strand with the help of the protein, the recombinase and the primer form a complex; perform a homology search and bind the target homology domain, at this time a D-loop region is formed at the homology positionand strand exchange begins; accompanied by the recombinase from the complex upon dissociation,the polymerase also bindsto the 3′ end of the primer,initiating chainextension.It is suitable for laboratory-level RNA amplification and RNA amplification for other detection purposes.

Isothermal nucleic acid Product Features

1/ High sensitivity and specificity, short reaction time.

2/ The reagent form is freeze-dried, stable and easy to operate.

3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.

Technical Parameters:

ParametersDetails
Product NameRNA Isothermal Amplification Kit Basic
ManufacturerAmp-future
Storage Temperature-20°C
Kit ComponentsEnzymes, Buffers ,Reagents
Packaging48 Tests/box
Detection Limit500-1000copies/µL
ShippingICE
Test Time5-20mins

Isothermal nucleic acid Applications

Suitable for RNA isothermal rapid amplification kit(BASIC type)

Primer: Require pair of nucleotide primers with the length of 25-35 bp.

RNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.

Notes

1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.

2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.

OT-2 Robot

The Opentrons® OT-2 is a high-precision, open-source liquid handler designed to be easy for scientists to use and flexible enough to automate many application workflows. This robot and its accompanying automation platform are used to automate hundreds of protocols and workflows in genomics, proteomics, cell-based assays, and drug discovery.

The OT-2 has a user-friendly, plug and play graphical interface to design protocols compatible with any ANSI/SBS compliant or automation friendly labware. The base model includes an Opentrons GEN2 Single-Channel Pipette; you can also use up to two pipettes at the same time. Additional pipettes can be purchased separately and are easily interchangeable by the user. Consumables and other labware are sold separately.