Overview
- Isolate high quality total RNA (including small RNA and microRNA) and all sizes of circulating and exosomal RNA, including microRNA from urine and cerebrospinal fluid (CSF) samples
- Small urine and CSF input ranging from as low as 0.5 mL to 1 mL
- No phenol extractions
- Very sensitive and linear down to a few cells without the need for carrier RNA
- Bind and elute all RNA irrespective of size or GC content, without bias
- Rapid and convenient spin column protocol
- Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix
Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (All sizes, including < 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease-specific outcomes, and prediction of therapeutic responses.
Details
Supporting Data
Figure 1 / 1
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| Kit Specifications | |
| Volume of Urine Processed | 0.5 – 1 mL |
| Size of RNA Purified | All sizes, including < 200 nt |
| Time to Complete 10 Purifications | < 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 29000 (25 preps) |
|---|---|
| Lysis Buffer A | 2 x 20 mL |
| Wash Solution A | 18 mL |
| Elution Solution A | 6 mL |
| Mini Spin Columns | 25 |
| Collection Tubes | 25 |
| Elution Tubes (1.7 mL) | 25 |
| Product Insert | 1 |
