Overview

• Ready-to-use
• Highly stable
• Precise
• 22 discrete bands from 60 bp to 600 bp

• High quality ready-to-use double-stranded DNA ladder derived by recombinant technology for precise size and mass determination in various fluorescence-detection instruments including different models of Applied Biosystems® PRISM® and Genetic Analyzers 
• 22 discrete bands, ranging from 60 bp to 600 bp
• The ladder is asymmetrically labelled with ROX that can be detected by excitation at 576 nm and fluorescent emission at 597 nm
• Compatible with products for fragment analysis including Promega PowerPlex® 16 System

Details

Supporting Data

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Ladder Properties:
• 22 discrete bands, ranging from 60 bp to 600 bp
 

Storage:
• Store at -20°C in small aliquots. For longer term storage, -70°C is recommended.
• The product is light-sensitive. Minimize light exposure.

GL20 Technical Parameter：

Max. Speed	21000rpm
Max. RCF	30910×g
Max. Capacity	4×800ml
Time Range	1~9h59min
RPM/RCF Convert	Yes
Noise (dB)	≤ 60
Temperature	-20℃~40℃
Acc/Dec	10 Kinds
Speed Accuracy	±20r/min
Temperature Accuracy	±1℃
Voltage(V/Hz)	AC 220V/110V 50HZ/60HZ
Size (W x D x Hmm)	680×540×830mm
Net Weight(Kg)	170KG
Certificates	CE,ISO & Calibration report are available

Matched Rotors for GL20

Order No	Rotor	Rotor Max Speed(r/min)	Volume(ml)	RCF(×g)
G20-1	Swing Rotor	4000	4×800ml	3450
G20-2	Microplate rotor	4000	4×4×96 well	2940
G20-3	Microplate rotor	4000	2×4×96 well	3210
G20-4	Microplate rotor	4000	2×3×48 well	2300
G20-5	Swing rotor	4000	4×40×7ml vacuum tube	3270
G20-6		4000	4×40×5ml vacuum tube	2890
G20-7		4000	4×28×10ml vacuum tube	3130
G20-8	Swing Rotor	4000	Fat bottle	3830
G20-9	Fixed Rotor	16000	4×8PCR	15760
G20-10	Fixed Rotor	15000	6×8PCR	21420
G20-11	Fixed Rotor	16000	8×8PCR	17480
G20-12	Fixed Rotor	15000	12×8PCR	22930
G20-13	Fixed Rotor	15000	40×0.5ml	22920
G20-14	Fixed Rotor	21000	12×1.5ml	30910
G20-15	Fixed Rotor	16000	24×1.5ml	23440
G20-16	Fixed Rotor	14000	30×1.5ml	20800
G20-17	Fixed Rotor	13000	48×1.5ml	17930
G20-18	Fixed Rotor	16000	16×5ml	22020
G20-19	Fixed Rotor	16000	6×10ml	21500
G20-20	Fixed Rotor	15000	12×10ml	22680
G20-21	Fixed Rotor	13000	16×10ml	19490
G20-22	Fixed Rotor	13000	8×15ml	17790
G20-23	Fixed Rotor	11000	12×15ml	14330
G20-24	Fixed Rotor	5000	24×15ml	3500
G20-25	Fixed Rotor	5000	30×15ml	3830
G20-26	Fixed Rotor	14000	6×30ml	19060
G20-27	Fixed Rotor	13000	6×50ml(conical)	18840
G20-28	Fixed Rotor	13000	6×50ml(round)	18730
G20-29	Fixed Rotor	5000	12×50ml	3860
G20-30	Fixed Rotor	4000	24×50ml	2970
G20-31	Fixed Rotor	13000	4×85ml	18940
G20-32	Fixed Rotor	12000	4×100ml	14850
G20-33	Fixed Rotor	10000	6×100ml	11380
G20-34	Fixed Rotor	4000	12×100ml	2970
G20-35	Fixed Rotor	12000	24 capillaries	15800
G20-36	Swing Rotor	15000	4×5ml	19920
G20-37	Vertical rotor	16000	16×5ml	16450
G20-38	Vertical rotor	14000	8×30ml	19750

Features

1. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.

2.Imported compressors fluorine free,double cycle cooling, cold and hot alternating easily, free environment pollution and precise in temperature control

3.Microprocessor control, digital display which indicates the temperature, speed, time, RCF in operation, speed raising and reducing quickly, operate simply. Running parameter can be edited.

4. 10 kinds of program stored in the memory, 10 kinds of accelerating and decelerating speed for your choice.

5. Automatically electric lid lock, over speed, over temperature protection and imbalance protection.

6.High quality steel centrifuge body , stainless steel chamber, built-in stainless steel explosion-proof protect inner sleeve , 3 tiers protection,safe and reliable.

7. Rotor is connected to spindle by specialized taper sleeve, loading simple and quick, no direction.

Introduction

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.

Details

Specifications

Features	Specifications
Main Functions	Recover DNA fragments >100bp from agarose gel(<0.5g), purification of DNA from PCR, enzymatic reaction solution or crude gDNA
Applications	PCR, NGS, labeling, ligation and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Agarose gel, PCR products, enzyme products
Sample amount	Agarose gel: ≤500mg
Recovery	≥80%
Elution volume	≥15μl
Time per run	≤20 minutes（1-24 samples）
Liquid carrying volume per column	800µl
Binding yield of column	35µg

Principle

The HiPure system uses a simple bind-wash-elute procedure. Gel slices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Advantages

• High recovery efficiency – ≥80% DNA recovery
• General – recover DNA from gel or enzyme-driven reaction solutions such as PCR
• Fast – isolation can be completed in 10-15 minutes by column gel method
• Great cost-effectiveness performance

Kit Contents

Contents	D211102	D211103
Purification Times	100 Preps	250 Preps
Buffer GDP	120 ml	250 ml
Buffer DW2	50 ml	2 x 50 ml
Elution Buffer	20 ml	30 ml
HiPure DNA Mini Columns II	100	250
2 ml Collection Tubes	100	250

Storage and Stability

The Kit should be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Experiment Data

HiPure Gel Pure DNA Kit uses proprietary chemistry and HiPure technology to recover DNA fragments between 60bp-10kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing, restriction digestion, or various labeling reactions. In addition, this kit can be also used to recover DNA directly from enzymatic reactions such as PCR and enzyme digestion reactions.