Name of Product

HIT – Heparin-Induced Thrombocytopenia Test (20)

Catalog Number

MQHIT 1

Short Info

Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

This product is only available in Germany!

Method/Platform

lateral flow, immunoassay

Range/Assay Sensivity

Test Principle

IgG antibodies are resposible for the heparin induced thrombocytopenia.

Immobilized anti-human IgG on the membrane of the test unit binds patients IgG-antibodies which are previously captured by the PF4/polyanion-complex which is detected by intensely colored gold nanoparticles.

The presence of PF4/polyanion-complex becomes visible at a colored test line. The surplus of gold particles continues to migrate through the membrane and is captured at the control line by specific antibodies.

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Lateral Flow Assay designed for the detection of IgG antibodies against PF4/polyanion-complexes in human citrated plasma or Serum

Overview

• Isolate all sizes of exosomal and extracellular vesicle RNA, including microRNA
• ​Bind and elute all RNA irrespective of size or GC content, without bias
• No phenol extractions
• No Proteinase K treatment
• No carrier RNA
• Concentrate isolated RNA into a flexible elution volume ranging from 50 µL to 100 µL
• Purify high-quality RNA in 15-20 minutes
• Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin

This kit provides a fast, reliable and convenient method to isolate and concentrate exosomal RNA from previously purified exosomes. It allows for the isolation of RNA from intact extracellular vesicles (EVs) purified from different urine or plasma/serum sample volumes. The purification is based on Norgen’s proprietary resin.

The Exosomal RNA Isolation Kit is designed to isolate all sizes of RNA, including microRNA. Moreover, the kit allows the user to elute into a flexible elution volume ranging from 50 µL to 100 µL. The purified RNA is of the highest integrity, and can be used in a number of downstream applications including real time PCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, and expression array assays.

Details

Supporting Data

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Kit Specifications
Sample type	Exosomes purified using Norgen’s Purification Kits and most other methods
Size of RNA Purified	All sizes, including miRNA and small RNA (< 200 nt)
Elution Volume	50-100 μL
Time to Complete 10 Purifications	35-40 minutes
Average Yields*	Variable depending on specimen

*Please check page 5 of the product insert for the average yields and the common RNA quantification methods.

Storage Conditions and Product Stability
All buffers should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment. It is recommended to warm Lysis Buffer A for 20 minutes at 60°C if any salt precipitation is observed.

Component	Cat. 58000 (50 preps)
Lysis Buffer A	20 mL
Lysis Additive B	2 mL
Wash Solution A	18 mL
Elution Solution A	6 mL
Mini Spin Columns	50
Collection Tubes	50
Elution Tubes (1.7 mL)	50
Product Insert	1

Introduction

HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.

Details

Specifications

Features	Specifications
Main Functions	Isolation total DNA from 150 mg plant and fungal tissue
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Fresh / frozen plant samples, dried plant / seed samples
Sample amount	Fresh / frozen plant samples: 50-150 mgDried plant / seed samples: 15-40 mg
Elution volume	≥40μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

This product is based on silica column purification. The sample is lysed with CATB Buffer. DNA is released into the lysate. Cell debris, precipitated proteins and polysaccharides are removed by chloroform extraction. After adjust the binding condition, transfer to an adsorption column. DNA is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• Broad spectrum – suitable for extracting DNA from various plant samples
• Fast – several samples can be extracted in 30 minutes by silica technology
• Good repeatability – silica gel column purification technology can obtain ideal results every time

Kit Contents

Contents	D318702	D318703
Purification Times	50 Preps	250 Preps
Buffer PAL	60 ml	200 ml
Buffer GWP	60 ml	200 ml
Buffer DW1	30 ml	150 ml
Buffer GW2*	20 ml	50 ml
Buffer AE	20 ml	60 ml
HiPure DNA Mini Columns II	50	250
2 ml Collection Tubes	50	250

Storage and Stability

This product can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Purchase Guide

Name	CAT NO	Fresh / frozen tissue amount	Dried tissue amount	Column type	Elution volume	Yield (muscle)
HiPure HP Plant DNA Kit	D3187	150mg	40mg	1.5ml column	50 – 100μl	3-70μg
HiPure HP Plant DNA Maxi Kit	D3163	5g	1g	50ml column	0.7 – 3ml	75-1250μg
HiPure SF Plant DNA Kit	D3164	100mg	20mg	1.5ml column	50 – 100μl	3-50μg
HiPure SF Plant DNA 96 Kit	D3167	50mg	15mg	96 well plate	75 – 150μl	3-30μg

HiPure Plant DNA Mini Kit supplies a simple and rapid extraction of genomic DNA from different plant samples. The kit is based on silica gel column and CTAB lysis purification technology. The whole extraction process is only 30-50 minutes. Purified DNA can be used directly for PCR, SSR, AFLP, RAPD and Southern Blot, etc.