This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.
Detail
Introduction
This product speeds and simplifies nucleic acid size selection for fragment library preparation for next generation sequencing.
Details
Specifications
Features
Specifications
Main Functions
Selectively recover DNA from PCR products and enzymatic reaction solution （Replace Beckmen or agencourt SPRISelect）
Applications
Prepare DNA library for second generation sequencing
Purification technology
Magnetic beads technology
Process method
Manual or automatic
Sample type
DNA products, restriction endonuclease systems, or other enzymatic reaction solution
Sample amount
Appropriate
Recovery
80%
Operation time
≤50 minutes
Principle
The MagSelect method contains magnetic particles in an optimized binding buffer to selectively bind DNA fragments 100bp and larger to paramagnetic beads. Excessprimers, nucleotides, salts, and enzymes can be removed using a simple washing procedure. The result is a more purified PCR product.
Advantages
High recovery – up to 80%
High throughput – using magnetic beads purification technology
Kit Contents
Contents
XP-5
XP-50
XP-500
MagSelect Beads
5 ml
50 ml
500 ml
Storage and Stability
MagSelect XP should be stored at 2-8°C upon arrival and is stable up to 18 months under the condition. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. Shake the reagent well before use. It should appear homogenous and consistent in color.
170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)
Content:
170.5 mL of prepared reagent (e.g. 550 assays of 0.31 mL)
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 2 years under recommended storage conditions
Analyte:
Acetic Acid
Assay Format:
Auto-analyser
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
up to 1.8 g/L of acetic acid per assay
Limit of Detection:
10 mg/L (recommended assay format)
Reaction Time (min):
~ 10 min
Application examples:
Wine, beer, fruit and fruit juices, soft drinks, vinegar, vegetables, pickles, dairy products (e.g. cheese), meat, fish, bread, bakery products (and baking agents), ketchup, soy sauce, mayonnaise, dressings, paper (and cardboard), tea, pharmaceuticals (e.g. infusion solutions), feed and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Improved method
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution.
View our complete list of organic acid assay kits.
Advantages
Very stable reagent when prepared for auto-analyser applications (> 7 days at 4oC)
PVP incorporated to prevent tannin inhibition
Linear calibration (R2 ~ 0.9995) up to 30 μg/mL of acetic acid in final reaction solution
Validated by the University of Wine, Suze la Rousse, France
Very rapid reaction
Very competitive price (cost per mL of reagent)
All reagents stable for > 2 years
Extended cofactors stability
Document
The Acetic Acid analyser format test kit is suitable for the specific measurement and analysis of acetic acid (acetate) in beverages and food products. On calibration, the prepared reagent is linear to > 28 micrograms of acetic acid per mL of assay solution.
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from 500 mg soil sample
Applications
RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Forest soil, grassland soil, mining area soil, sediment and other samples
Sample amount
500 mg
Elution volume
≥30μl
Time per run
≤60 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
High quality – high purity total RNA can be directly used in various sensitive downstream applications
Fast – extraction of several samples can be completed in 60 minutes by column purification method
Safe – no phenol chloroform extraction required
Sensitive – RNA can be purified at the level of PG
Kit Contents
Contents
R418302
R418303
Purification Times
50 Preps
250 Preps
HiPure RNA Micro Columns
50
250
2ml Collection Tubes
100
500
gDNA Filter Column
50
250
2ml Beads Tubes
50
250
Buffer SOL
30 ml
150 ml
Buffer SDS
4 ml
15 ml
Buffer PHC
30 ml
150 ml
Buffer GDP
40 ml
150 ml
Buffer RW1
50 ml
200 ml
Buffer RW2 *
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
Buffer PHC should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15–25°C) and are stable for at least 18 months under theseconditions.The entire kit can be stored at 2–8°C, but in this case buffers should be redissolvedbefore use. Make sure that all buffers are at room temperature when used.
Experiment Data
Document
HiPure Soil RNA Kit is suitable for extracting high-purity microbial total RNA from soil samples. The kit adopts silica gel column purification technology and original humic acid adsorbent technology. It is suitable for extracting high-yield and high-purity total RNA from various soil samples, such as forest soil, grassland soil, mining soil, sediment and so on. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
