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A3P SCIENTIFIC
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A3P (ATP) acts as a powerhouse of energy, driving transformation and innovation, enabling new and better possibilities for growth and advancement.
Authorized distributor
3CR Bioscience Limited’s patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent results.
D2141 HiPure DNA Clean Up Kit
Introduction
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
Details
Specifications
| Features | Specifications |
| Main Functions | Recover DNA fragments between 20bp-20kbp from crude DNA and enzymatic reaction solution |
| Applications | PCR, sequencing, labeling reactions, ligations and restriction digestion, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Crude DNA, enzymatic reaction solution |
| Sample amount | <150μl |
| Recovery | 80% |
| Elution volume | ≥15μl |
| Time per run | ≤10 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 20µg |
Principle
The HiPure system uses a simple bind-wash-elute procedure. Gelslices are dissolved in a buffer containing a pH indicator, allowing easy determination of the optimal pH for DNA binding, and the mixture is applied to the column. Nucleic acids adsorb to the silica-gel membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.
Advantages
- High recovery efficiency – up to 80% DNA recovery
- Fast – isolation can be completed in 10 minutes by column gel method
- Low elution volume – elution volume can be as low as 10μl
- Efficient – efficient removal of various inhibitory factors
Kit Contents
| Contents | D214102 | D214103 |
| Purification Times | 100 Preps | 250 Preps |
| Buffer GWP | 30 ml | 60 ml |
| Buffer DW2 | 20 ml | 50 ml |
| Elution Buffer | 30 ml | 60 ml |
| HiPure DNA Mini Columns I | 50 | 250 |
| 2 ml Collection Tubes | 50 | 250 |
Storage and Stability
HiPure DNA Clean Up Kit components are guaranteed for at least one year when stored at room temperature. If any precipitates form in the buffers, warm at 37℃ to dissolve.
HiPure DNA Clean Up Kit usesproprietary chemistry and HiPure technology to recover DNA Fragments between20bp-20kbp with yields exceeding 80%. DNA is suitable for ligations, PCR, sequencing,restriction digestion, or various labeling reactions. In addition, this kit canbe also used to recover DNA directly from enzymatic reactions such as PCR andenzyme digestion reactions.
R4311 HiPure Cell miRNA Kit
Introduction
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total RNA and miRNA from cell and tissue without MagZol reagent |
| Applications | RT-PCR, Northern Blot, poly A+purification, nucleic acid protection and in vitro translation |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Cell, Animal tissue, Plant tissue |
| Sample amount | Cells: ≤ 5 x 10^6, Animal tissue:<10mg |
| Elution volume | ≥30μl |
| Time per run | ≤40 minutes |
| Liquid carrying volume per column | 800µl |
| Binding yield of column | 100µg |
Principle
Biological samples are first lysed and homogenized in a highly denaturing guanidine isothiocyanate-containing buffer, which immediately inactivates DNases and RNases to ensure isolation of intact DNA and RNA. The lysate is then passed through a Mini spin column. This column, in combination with the high-salt buffer, allows selective and efficient binding of genomic DNA. Flow-through from the column is digested by Proteinase K in the presence of ethanol. This optimized digestion, together with the subsequent addition of further ethanol, allows appropriate binding of total RNA, including miRNA, to the column. Contaminants are efficiently washed away and high-quality RNA is eluted.
Advantages
- High quality – one step RNA extraction reagent combined with silica gel column can obtain the highest concentration
- Fast – several samples can be extracted in 40 minutes
- High applicability – samples including animals, plants, bacteria, cells, etc.
- High concentration – efficiently remove macromolecular RNA, enrich small RNA and improve sensitivity
- Safe – no phenol chloroform extraction and no use of Trizol reagent
Kit Contents
| Contents | R431102 | R431103 |
| Purification Times | 50 Preps | 250 Preps |
| HiPure RNA Mini Columns | 100 | 2 x 250 |
| 2ml Collection Tubes | 100 | 2 x 250 |
| Proteinase K | 48 mg | 240 mg |
| Protease Dissolve Buffer | 5 ml | 15 ml |
| Buffer RLC | 40 ml | 200 ml |
| Buffer RWC | 20 ml | 80 ml |
| Buffer RW2* | 20 ml | 2 x 50 ml |
| RNase Free Water | 10 ml | 60 ml |
Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
The Kit is designed for purification of total RNA, including miRNA and other small RNA molecules (18nt), from cultured cells and various animal and human tissues, including difficult-to-lyse tissues samples.
Streptavidin Europium Chelate Microspheres For Lateral Flow
- Ready to Use Streptavidin Europium Chelate Microspheres
- Specifically Designed Running Buffer
Description
Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow
Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres can irreversibly bind biotin.
Europium dyed polystyrene beads conjugated to streptavidin
250ul of a 1mg/ml stock ready to use for Lateral Flow
50mL Lateral FLow Running Buffer designed for Streptavidin Europium Chelate Microspheres
Inquire for Larger Volumes: [email protected]
Excitation max: 365nm
Emission max: 610nm
Europium Chelate Microspheres Nanoparticle Streptavidin Conjugate for Lateral Flow
Our Streptavidin Europium Chelate Microspheres conjugate is manufactured using special conjugation technology and functonally tested by lateral flow. We have coated our high quality nanoparticles with a proprietary surface coat that covalently binds the biotin forming ultra stable conjugates. The resulting Streptavidin Europium Chelate Microspheres can irreversibly bind biotin.
IVD6672 MagPure Pathogen DNA/RNA Kit
Introduction
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
Details
Specifications
| Features | Specifications |
| Main Functions | Extract total pathogen nucleic acid from cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant. |
| Applications | RT-PCR，PCR |
| Products | Pathogen DNA / RNA |
| Purification method | Polydisperse magnetic beads |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | cell-free/low-content cell biological samples such as body fluids, serums, plasma, tissue homogenate supernatant |
| Sample amount | 200-300μl |
| Adaptive instrument | Nucleic acid extractor, pipetting workstation |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. After adding magnetic particles and binding solution, DNA/RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer NFW.
Advantages
- Fast – several samples can be extracted in 40 minutes by column method
- High quality – high purity total RNA / DNA can be directly used in various sensitive downstream applications
- Safe – no phenol chloroform extraction required
- Sensitive – DNA/RNA can be recovered at the level of PG
Kit Contents
| Contents | IVD6672-50 | IVD6672 |
| Purification Times | 50 Preps | 200 Preps |
| Bead Tube C | 50 | 4 x 50 |
| MagPure Particle | 1.6 ml | 7.0 ml |
| Proteinase K | 24 mg | 100 mg |
| Protease Dissolve Buffer | 1.8 ml | 6 ml |
| Buffer MLBN | 30 ml | 120 ml |
| Buffer MW1* | 22 ml | 53 ml |
| Buffer MW2* | 20 ml | 50 ml |
| Buffer NFW | 10 ml | 30 ml |
Storage and Stability
MagPure Particles and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage (up to 8 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for 18 months under these conditions.
This product is suitable for extracting total pathogen nucleic acid from biological samples with no/low cell content such as body fluid, serum, plasma, soaking solution, tissue homogenate supernatant, culture medium supernatant, etc. The purified DNA/RNA can be used for clinical in vitro detection.
D6352 MagPure Seed DNA Kit
Introduction
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed |
| Applications | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Conventional economic plant samples |
| Sample amount | 50-100mg |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
- Safe – require no phenol or chloroform extraction
- Fast – several samples can be extracted in 60 minutes
- High purity – high quality DNA, completely remove inhibitors
Kit Contents
| Contents | D635201 | D635202 | D635203 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 1.7 ml | 4 ml | 18 ml |
| Buffer SOL | 40 ml | 90 ml | 500 ml |
| Buffer SDS | 4 ml | 9 ml | 50 ml |
| Buffer MPB | 40 ml | 70 ml | 350 ml |
| Buffer GW1 | 26 ml | 53 ml | 220 ml |
| Elution Buffer | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Microcystin Detection Kit (Rapid – Recreational Water)
- Screening of Microcystins in water samples at 6 ppb or lower
- Format: 10 tests (5 tests/5 control)
- Water Sample Bottles
- Negative Control
- 1mL Syringe
- Sample Dilution Buffer also sold separately
- Sample Filters
Description
Attogene’s Microcystin Test Kit (Rapid – Recreational Water) can be used to detect microcystins in liquid samples; highly sensitive, rapid, robust screening kit for algae toxins microcystins and nodularins.
The most frequently reported cyanobacterial toxins are the hepatotoxic microcystins (MCs). MCs are peptides with a molecular weight ranging from 900 to 1,100 Da. They consist of seven amino acids of which the two terminal amino acids of the linear peptide are condensed to form a cyclic compound.
A tiered notification system which takes different actions based on different numeric thresholds for microcytin-LR concentrations in recreational waters has been developed. This is guidance that allows states to take various actions—such as posting information about harmful algal blooms (HABs), issuing a recreational public health advisory, or temporarily closing recreational waters through a no contact advisory—depending on the severity of the bloom event.
Microcystin-LR
Informational sign postings about HABs at recreational waters: < 6 μg/L
Recreational public health advisory: 6 μg/L
Elevated recreational public health advisory (e.g. no contact): 20 μg/L
Screening of Microcystins in water samples at 6 ppb or lower
Format: 10 tests (5 tests/5 control)
Water Sample Bottles
Negative Control
1mL Syringe
Sample Dilution Buffer also sold separately
Sample Filters
Our valued customer
Our Team
Our sales team is comprised of knowledgeable and experienced individuals in the field of science, who are committed to service, honesty, and responsibility. We strive to ensure that our customers receive unparalleled service that they won’t find anywhere else. We want our customers to feel confident that we will provide them with the very best service possible.
TANATHORN VITISANT
Sales manager
Phone : 081-875-1869
Email : [email protected]
Line id : @a3p-scientific
NUCHANAT JANPRAPAS
Area Sales Manager
Phone : 099-263-6624
Email : [email protected]
Line id : belongkong
NANTASAK SRISUWAN
Area Sales Manager
Phone : 094-562-5914
Email : [email protected]
Line id : north6906295
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