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3CR Bioscience Limited’s patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent results.
DBCO-PEG4-PFP ester
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-PEG4-PFP ester is a PFP active ester containing a DBCO group which can undergo copper-free Click Chemistry with azide (N3). The PFP ester moiety is reactive with amine groups. PFP esters have been are stable compounds and are less susceptible to undergo hydrolysis. The hydrophilic PEG linker increases the water solubility of a compound in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HCM116 Slanetz and Bartley Medium
Introduction
Intended Use
For the enumeration of enterococci in water and other liquids by the membrane filtration technique.
Principle and Interpretation
The growth of the entire accompanying Gram-negative microbial flora is inhibited by sodium azide. Enterococci reduce 2,3,5-Triphenyl tetrazolium chloride (TTC) to give a red formazan inside the bacterial cell, their colonies are thus red. Nitrogen, minerals, and amino acids are provided by the tryptose whilst yeast extract supplies vitamins. Glucose acts as the carbon source, dipotassium phosphate buffers the medium, and agar-agar is the solidifying agent.
Formulation
| Ingredients | /liter |
| Tryptose | 20.0g |
| Yeast extract | 5.0g |
| Glucose | 2.0g |
| Disodium hydrogen phosphate | 4.0g |
| Sodium azide | 0.4g |
| 2,3,5-Triphenyl Tetrazolium chloride | 0.1g |
| Agar | 10.0g |
| pH 7.2±0.1 at 25°C | |
Preparation
Dissolve 41.5 g in 1 L of purified water. Heat in boiling water and agitate frequently until completely dissolved. Sterilize by further heat for 20 minutes in the boiling water bath.
Quality Control
Cultural characteristics observed after incubation at 34-38°C for 40-48hours
| Quality control strains | Growth | Colony color |
| Enterococcus faecalis ATCC29212 | Good growth,PR≥0.5 | deep red coloured colonies |
| Escherichia coli ATCC25922 | Total inhibition | – |
Sorage and Shelf Life
Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.
Precautions
1. When weighing the dehydrated medium, please wear masks to avoid causing respiratory system discomfort
2. Keep container tightly closed after using to prevent clumping.
Waste Disposal
Microbiological contamination was disposed by autoclaving at 121°C for 30 minutes.
Intended Use For the enumeration of enterococci in water and other liquids by the membrane filtration technique. Principle and Interpretation The growth of the entire accompanying Gram-neg……
5-20mins High Sensitivity Isothermal PCR Kit NFO Short Reaction Time
Product Description
High Sensitivity Isothermal PCR Kit NFO | Specificity, Short Reaction Time | 30-37℃ | 10-100 CFU/reaction
Product Detail
Kit Storage and term of Validity
Storage term: stored at ≤-20℃,keep away from light, avoid heavy weight and repeated freezing and thawing.
Term of Validity: 14 months
Isothermal nucleic acid Principle Summary
The kit is based on room and constant temperature nucleic acid rapid amplification technology, its principle is that at room and constant temperature, the recombinase and primer form a protein/single-stranded nucleotide complex Rec/ssDNA, and invade the double-stranded DNA template with the help of auxiliary proteins and single-stranded binding protein SSB; then form a D-loop region at the invasion point and start to scan the DNA duplex, after finding the target region complementary to the primer and disintegration of the complex Rec/ssDNA, the polymerase also binds to the 3′ end of the primer to start the chain extension. The kit relies on the role of NFO enzyme and adds the designed specific molecular probes according to the template, and get the result by colloidal gold technology (sandwich method).
Isothermal nucleic acid Product Features
1/ High sensitivity and specificity, short reaction time.
2/ The reagent form is freeze-dried, stable and easy to operate.
3/ The reaction can be operated by metal bath and water bath pot without purchasing expensive PCR apparatus.
Technical Parameters:
| Parameters | Details |
|---|---|
| Product Name | DNA Isothermal Amplification Kit NFO |
| Manufacturer | Amp-future |
| Storage Temperature | -20°C |
| Kit Components | Enzymes, Buffers ,Reagents |
| Packaging | 48 Tests/box |
| Detection Limit | 500-1000copies/µL |
| Shipping | ICE |
| Test Time | 5-20mins |
Isothermal nucleic acid Applications
Suitable for DNA isothermal rapid amplification kit(NFO type)
Primer: Require pair of nucleotide primers with the length of 25-35 bp.
DNA basic kit reaction temperature is 39 to 42℃ and time is 5-20 minutes.
Notes
1/ Please avoid nucleic acid contamination and set blank control during reaction due to the high sensitivity of the kit.
2/ Please take out the required quantity of MIRA reaction units for the experiment, and put the rest under storage conditions when performing the experiment.
Propargyl-PEG10-acid
Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Propargyl-PEG10-acid is a propargyl PEG linker with a terminal carboxylic acid. The terminal carboxylic acid forms amide bonds with primary amines; activators (EDC or HATU) will be needed. The propargyl group can react with azide-bearing compounds to form stable triazole bonds. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Total RNA Purification Kits
Overview
- Extract high quality & quantity total RNA including miRNA
- No phenol step required; isolate all RNA in one fraction
- Bind & elute all RNA irrespective of size or GC content, without bias
- Very sensitive & linear down to a few cells without the need for carrier RNA
- Isolate from a wide variety of specimens
- Purified RNA is suitable for a variety of downstream applications, including Small RNA Sequencing. Find out more information on Norgen’s NGS services
- Available in a variety of formats to suit your needs
- Purification is based on spin column chromatography that uses Norgen’s resin separation matrix
These kits are suitable for the isolation of total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more. Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more. These kits purify all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol. Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.
Total RNA Purification 96-Well Kit (High Throughput and High Throughput Deep Well)
This 96-well kit provides a rapid method for the high-throughput isolation and purification of total RNA in 30 minutes using vacuum manifold, plate centrifuge, or liquid handlers with vacuum capabilities. Total RNA can be isolated from a broad range of sample sources including cultured cells, tissues, blood, serum, plasma, bacteria, yeast, fungi, and viruses.
Isolate RNA after Purifying EVs and Exosomes
Ultracentrifugation, Exoquick, Filtration
| Cat. # | Name | Elution Volume | Plasma/Serum | Urine | Cell-Culture Media |
|---|---|---|---|---|---|
| 55000 | Plasma/Serum RNA Purification Mini Kit | 10 – 25 µL | 50 µL – 1 mL | 250 µL – 1 mL | 5 – 10 mL |
| 35300 | Total RNA Purification Micro Kit | 20 – 50 µL | 1 – 4 mL | 2 – 10 mL | 10 – 20 mL |
| 17200 | Total RNA Purification Kit | 50 – 100 µL | 4 – 10 mL | 11 – 30 mL | 20 – 35 mL |
Details
Supporting Data
Figure 1 / 13
Click for expanded view
| Kit Specifications | |
| Maximum Binding Capacity | Up to 50 μg RNA |
| Maximum Loading Volume | 650 μL |
| Size of RNA Purified | All sizes, including small RNA (< 200 nt) |
| Maximum Amount of Starting Material | |
| Animal Cells | 3 x 106 cells |
| Animal Tissues | 10 mg (for most tissues*) |
| Blood | 100 μL |
| Plasma/Serum | 200 μL |
| Bacteria | 1 x 109 cells |
| Yeast | 1 x 108 cells |
| Fungi | 50 mg |
| Plant Tissues | 50 mg |
| Time to Complete 10 Purifications | 20 minutes |
| Average Yield | |
| HeLa Cells (1 x 106 cells) | 15 μg |
| E. coli (1 x 109 cells) | 50 μg |
* for isolating total RNA from larger amounts of tissue, please use Norgen’s Animal Tissue RNA Purification Kit (Cat# 25700)
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. These kits are stable for 2 years after the date of shipment.
| Component | Cat. 17200 (50 preps) | Cat. 37500 (100 preps) | Cat. 17250 (250 preps) | Cat. 17270 (500 preps) | Cat. 24300 (192 preps) | Cat. 24370 (576 preps) | Cat. 24350 (192 preps) | Cat. 24380 (576 preps) |
|---|---|---|---|---|---|---|---|---|
| Buffer RL | 40 mL | 2 x 40 mL | 175 mL | 350 mL | 2 x 40 mL | 350 mL | 2 x 40 mL | 350 mL |
| Wash Solution A | 38 mL | 2 x 38 mL | 148 mL | 1 x 148 mL 1 x 74 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL | 2 x 38 mL | 1 x 74 mL 1 x 148 mL |
| Elution Solution A | 6 mL | 2 x 6 mL | 30 mL | 60 mL | 2 x 20 mL | 60 mL | 2 x 20 mL | 60 mL |
| Mini Spin Columns | 50 | 100 | 250 | 500 | – | – | – | – |
| 96-Well Isolation Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Isolation Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Adhesive Tape | – | – | – | – | 4 | 12 | 4 | 12 |
| Collection Tubes | 50 | 100 | 250 | 500 | – | – | ||
| 96-Well Collection Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Collection Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Elution Tubes (1.7 mL) | 50 | 100 | 250 | 500 | – | – | ||
| 96-Well Elution Plate | – | – | – | – | 2 | 6 | – | – |
| 96-Well Elution Plate (Deep Well) | – | – | – | – | – | – | 2 | 6 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Our valued customer
Our Team
Our sales team is comprised of knowledgeable and experienced individuals in the field of science, who are committed to service, honesty, and responsibility. We strive to ensure that our customers receive unparalleled service that they won’t find anywhere else. We want our customers to feel confident that we will provide them with the very best service possible.
TANATHORN VITISANT
Sales manager
Phone : 081-875-1869
Email : [email protected]
Line id : @a3p-scientific
NUCHANAT JANPRAPAS
Area Sales Manager
Phone : 099-263-6624
Email : [email protected]
Line id : belongkong
NANTASAK SRISUWAN
Area Sales Manager
Phone : 094-562-5914
Email : [email protected]
Line id : north6906295
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