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3CR Bioscience Limited’s patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent results.
GeneAb™ PSA
Description
Specifications
| Clone | IHC654 |
| Source | Mouse Monoclonal |
| Positive Control | Prostate, Prostate Carcinoma |
| Dilution Range | 1:200 |
Prostate-Specific Antigen (PSA) is a serine protease of the kallikrein family, that is produced by the prostate epithelium and epithelial lining of the periurethral glands. Although considered prostate-specific, PSA has also been detected in breast tissue, breast tumors, endometrium, adrenal neoplasms, and renal cell carcinomas. Anti-PSA can be used for differentiating high-grade prostate adenocarcinoma from high-grade urothelial carcinoma, as well as for determining the prostatic origin of carcinomas in non-prostate tissues. Anti-PSA recognizes primary and metastatic prostatic neoplasms, but not tumors of nonprostatic origin, and can be useful as an aid to confirm prostatic acinar cell origin in primary and metastatic carcinomas.
Blood DNA Isolation Kits
Overview
- High yield and high purity DNA ready for any application
- Available in a variety of formats to properly suit your needs
- Compatible with blood collected on a variety of commercially available tubes
These kits allow for the isolation of DNA from the blood of various species, including humans and will recover genomic DNA, mitochondrial DNA, viral DNA and bacterial DNA. The purified DNA is of excellent quality and yield and completely compatible with any downstream application including PCR, qPCR and genotyping.
Blood DNA Isolation Mini Kit
Norgen’s Blood DNA Isolation Mini Kit is designed for the rapid preparation of DNA from up to 200 µL of whole blood using a rapid spin column protocol. Preparation time for a single sample is less than 30 minutes and each kit contains sufficient materials for 50 preparations.
Blood DNA Isolation Midi Kit
Norgen’s Blood DNA Isolation Kit is designed for the rapid spin column preparation of DNA from 0.3 to 2 mL volumes of whole blood. Preparation time for a single sample is less than 30 minutes, and each kit contains sufficient materials for 20 preparations.
Blood DNA Isolation Maxi Kit
This kit is designed for the rapid preparation of DNA from 3 mL up to 10 mL of whole blood. Preparation time for a single sample is less than 30 minutes.
Blood DNA Isolation 96-Well Kit (High Throughput)
This kit provides a rapid method for the high-throughput isolation of DNA from up to 200 µL of whole blood. Fast and easy processing using either a vacuum manifold or centrifugation.
Blood DNA Isolation Kit (Magnetic Bead System)
Norgen’s Blood DNA Isolation Kit (Magnetic Bead System) is designed for the rapid preparation of genomic DNA from up to 200 µL of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgen’s magnetic beads bind DNA under optimized salt concentrations and releases the bound DNA under low salt and slightly alkali conditions. The genomic DNA is preferentially purified from other cellular proteinaceous components. Typical yields of genomic DNA will vary depending on the cell density of the blood sample. The purified genomic DNA is fully digestible with all restriction enzymes tested, and is completely compatible with downstream applications including real-time PCR, NGS and microarray analysis.
Blood DNA Isolation 96-Well Kit (High Throughput Magnetic Bead System)
96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Details
Supporting Data
Figure 1 / 19
Click for expanded view
| Kit Specifications | |
| Minimum Blood Input | 20 µL |
| Maximum Blood Input | 200 µL |
| Column Binding Capacity | > 50 µg |
| Average Yield (200 µL of blood) | 4-12 µg* |
| Time to Complete 10 Purifications | 30 minutes |
*Yield will vary depending on the type of blood processed
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment. The kit contains a ready-to-use Proteinase K, which is dissolved in a specially prepared storage buffer. The buffered Proteinase K is stable for up to 1 year after the date of shipment when stored at room temperature.
| Component | Cat. 46300 (50 preps) | Cat. 46380 (100 preps) | Cat. 51400 (20 preps) | Cat. 31200 (12 preps) | Cat. 46350 (192 preps) | Cat. 59800 (50 preps) | Cat. 62600 (192 preps) |
|---|---|---|---|---|---|---|---|
| Lysis Buffer B | 20 mL | 2 x 20 mL | 2 x 40 mL | 2 x 110 mL | 2 x 40 mL | 20 mL | 1 x 40 mL 1 x 20 mL |
| Solution WN | 18 mL | 2 x 18 mL | 55 mL | 55 mL | 55 mL | 18 mL | 55 mL |
| Wash Solution A | 18 mL | 2 x 18 mL | 2 x 38 mL | 2 x 38 mL | 2 x 38 mL | – | – |
| Elution Buffer B | 30 mL | 2 x 30 mL | 30 mL | 30 mL | 2 x 30 mL | 15 mL | 1 x 30 mL 1 x 15 mL |
| Proteinase K in Storage Buffer | 1.2 mL | 2 x 1.2 mL | 4.4 mL | 8 mL | 4.5 mL | 1.2 mL | 4 mL |
| Magnetic Bead Suspension | – | – | – | – | – | 2 x 1.1 mL | 8.5 mL |
| Maxi Spin Columns | – | – | – | 12 | – | – | – |
| Mini Spin Columns | 50 | 100 | – | – | – | – | – |
| Midi Spin Columns | – | – | 20 | – | – | – | – |
| 96-Well Plate | – | – | – | – | 2 | – | 2 |
| Adhesive Tape | – | – | – | – | 4 | – | 2 |
| Collection Tubes | 50 | 100 | 20 | 12 | – | – | – |
| 96-Well Collection Plate | – | – | – | – | 2 | – | – |
| Elution Tubes | 50 | 100 | 20 | 12 | – | 50 | – |
| 96-Well Elution Plate | – | – | – | – | 2 | – | 2 |
| Product Insert | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
N-(endo-BCN-PEG2-amido-PEG3)-N-bis-(PEG3-Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane)
N-(endo-BCN-PEG2-amido-PEG3)-N-bis-(PEG3-Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is is a multi-functional PEG linker with six terminal propargyl groups and a BCN group. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The BCN group can react with azide -tagged compound or biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(endo-BCN-PEG2-amido-PEG3)-N-bis-(PEG3-Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is is a multi-functional PEG linker with six terminal propargyl groups and a BCN group. The propargyl groups enables formation of triazole linkage with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry. The BCN group can react with azide -tagged compound or biomolecules. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
HiDi® 2x PCR Master Mix
- HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and therefor, is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).HiDi® 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only target-specific primers and template need to be added as the mix contains all components for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors.This PCR mix is also available with a full-length Taq DNA polymerase with a nuclease domain, featuring 100% compatibility with hydrolysis probes (TaqMan® probes etc.).Benchmarking with products of competitors conducted by us and others show that the HiDi® DNA polymerase family is the first choice for highly selective PCRs, such as genotyping by allele-specific PCR, HLA genotyping, analysis of single CpG methylation sites or the detection of mutations in a high background of wild-type sequences. By using HiDi® DNA polymerase, less than 10 copies of a mutation can be detected in a background of >10.000 wild-type copies straight away without any other tedious assay optimization.It has also been shown that HiDi® DNA polymerase family is highly suitable for quality control in CRISPR-Cas or TALEN-based applications.Several independently conducted studies show that HiDi® DNA polymerase is ideally suited for use in asPCR in numerous research areas ranging from mutation detection to genome editing. (read more)For research use and further manufacturing.In case you are aiming to use our RUO products as components or for your development of e.g. an IVD medical device, please contact us.
HiDi is available as:
HiDi® DNA Polymerase (>>)
HiDi® Taq DNA Polymerase (>>)
HiDi® 2x PCR Master Mix (>>)
HiDi® Taq 2x PCR Master Mix (>>)
Casestudies:
HiDi® DNA Polymerase: Applications from mutation detection to genome editing (read more)
Example Primer Design
Matching vs. mismatching nucleotide is placed at the 3′-end of the primer for best discrimination results.
Example Results – There´s no accounting for taste
Cilantro: some people love it in their food, some hate it. Here we are detecting a genomic SNP (rs72921001) in HeLa genomic DNA. This SNP is reported to be close to a number of genes coding for olfactory receptors. (Reference: Eriksson N. et al. (2012), “A genetic variant near olfactory receptor genes influences cilantro preference.”)
Considering, that only the C-allele specific primer is extended and yielding in a specific amplicon, we can conclude a genetic predisposition in disliking cilantro, as this SNP is significantly associated with detecting a soapy taste to cilantro.
Allele-specific PCRs were performed from 1 ng/µl of HeLa gDNA in the presence of a realtime dye, indicating the amplification of the C-allele specific primer only. The A-allele specific primer is discriminated, thus not amplified up to 50 cycles.
PCR products were subsequently analysed on a 2.5% agarose gel. Specific product is visualized by ethidium bromide staining at the amplicon length of 109 bp.
HiDi® stands for High Discrimination of mismatches at the 3’-terminus of primers in PCR. This myPOLS Biotec enzyme family is optimized for this feature and therefor, is the first choice for applications that rely on this property such as allele-specific PCR (asPCR) that is also termed allele-specific amplification (ASA).
HiDi® 2x PCR Master Mix – ready to use mix simplifies your PCR setup. Only target-specific primers and template need to be added as the mix contains all components for a successful and reliable PCR. This ensures reproducible results, significantly reduces set-up times and the risk of pipetting errors.
C1414 MagPure Particles F
Introduction
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
Details
Specifications
| Features | Specifications |
| Concentration | 50 mg/ml |
| Appearance | Suspension of dark brown particles |
| Surface functional group | Si-OH, Silanol |
| Dispersibility | Monodisperse,spherical |
| Particle size | 0.2-1.5 μm |
| Preservation conditions | Room Temperature, valid for up to 2 years.It is recommended to store in 2-8°C to prevent microbial growth. |
| Magnetic response speed | 20 seconds |
| Settling velocity | >3 minutes |
| High salt mediated binding | >2M guanidine isothiocyanate, DNA recovery up to 80% |
| Alcohol mediated binding | 2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85% |
| PEG8000 mediated binding | The recovery of DNA/RNA was up to 85% |
| DNase/RNase | Not detected |
| DNA residue | Not detected |
| Recommended application | Plasmid extraction,gel DNA recovery, genomic DNA extraction, RNA extraction, viral nucleic acidextraction, circulating DNA isolation |
Principle
Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.
Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.
After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.
Ordering information
| CAT.No. | Product Name | Package |
| C14140 | MagPure Particles F | 100 ml |
| C14141 | MagPure Particles F | 400 ml |
| C14142 | MagPure Particles F | 3 x 400 ml |
| C14143 | MagPure Particles F | 10 x 400 ml |
Purchase Guide
| Features | MagPure Particles | MagPure Particles N | MagPure Particles G | MagPure Particles F | MagBind Particles |
| Cat.No. | C1410 | C1411 | C1412 | C1414 | C1413 |
| Concentration | 100mg/ml | 70mg/ml | 40mg/ml | 50mg/ml | 10mg/ml |
| Form | Amorphous and Porous | Amorphous and Porous | Porous | Amorphous | Nonporous |
| Surface function | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | Si-OH, Silica Beads | COOH, Carboxyl Beads |
| Dispersion | Polydisperse | Polydisperse | Monodisperse | Monodisperse | Monodisperse |
| Particle Size | 1.5-5μm | 0.2-2μm | 1-1.5μm | 0.2-1.5μm | 0.8-1μm |
| Color | Black | Yellowish Brown | Dark Brown | Dark Brown | Yellowish Brown |
| Magnetic response | 15-30s | ~60s | ~30s | 20s | 120s |
| Settling Time (1ml) | >5min | >10min | >3min | >3min | >2h |
| Usage (0.2ml Sample) | 20μl | 20μl | 20-30μl | 20-30μl | 20-30μl |
| DNA Recover Rate (only 4M GITC) | >80% | >80% | >80% | >80% | 0 |
| DNA Recover Rate (10% PEG8000/NaCl) | >85% | >85% | >85% | >85% | >90% |
| Recommended Use | gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc.Viral DNA/RNA IsolationAgarose Gel DNA Purification | DNA/RNA Isolation from low nucleic acid content samplesPlasmid IsolationDNA/RNA Clean Up | Circulating DNA IsolationViral Nucleic acid IsolationgDNA Isolation FFPE DNA/RNA Isolation | Plasmid extractiongel DNA recoverygenomicDNA/RNA extraction viral nucleic acid extractionCirculating DNA extraction | DNA/RNA Clean Up and concentrationDNA/RNA Isolation from low nucleic acid content samplesResearch immuno assays |
- The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.
Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid. Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method. Magpure particle is a kind of polydisperse fast speed silica magnetic beads. The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.
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Our Team
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TANATHORN VITISANT
Sales manager
Phone : 081-875-1869
Email : [email protected]
Line id : @a3p-scientific
NUCHANAT JANPRAPAS
Area Sales Manager
Phone : 099-263-6624
Email : [email protected]
Line id : belongkong
NANTASAK SRISUWAN
Area Sales Manager
Phone : 094-562-5914
Email : [email protected]
Line id : north6906295
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