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3CR Bioscience Limited’s patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent results.
Giardia intestinalis TaqMan RT-PCR Detection Kits
Overview
- Detection kits for Giardia intestinalis
- CE-IVD marked version available for in vitro diagnostic use
- Available in TaqMan format for analysis.
Giardiasis is a disease of the small bowel caused by the protozoan parasite Giardia intestinalis (syn.duodenalis or lamblia). Giardia is one of the most common intestinal parasites in the world, and occurs at very high prevalence rates in places with poor water sanitation. Individuals become infected through ingesting or coming into contact with contaminated water, food or soil. It can also be spread through the faecal-oral route due to poor hygiene practices, which makes it common in day-care centers. G. intestinalis lives inside the intestines of infected humans or other animals including cats, dogs, birds, cows, beaver and deer. Symptoms of infection include diarrhea, malaise, excessive gas, bloating, nausea, diminished interest in food, possible vomiting and weight loss.
There are 2 kits available for the detection of Giardia intestinalis:
Giardia intestinalis TaqMan RT-PCR Kit, 100 reactions
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
Giardia intestinalis TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
Details
Supporting Data
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival.
| Component | Cat. TM43850 (100 preps) | Cat. TM43810 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| Giardia intestinalis Primer & Probe Mix | 280 μL | 280 μL |
| Giardia intestinalis Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
Internal Lane Standard (60bp – 600bp, ROX) for ABI Genetic Analyzer
Overview
- Ready-to-use
- Highly stable
- Precise
- 22 discrete bands from 60 bp to 600 bp
- High quality ready-to-use double-stranded DNA ladder derived by recombinant technology for precise size and mass determination in various fluorescence-detection instruments including different models of Applied Biosystems® PRISM® and Genetic Analyzers
- 22 discrete bands, ranging from 60 bp to 600 bp
- The ladder is asymmetrically labelled with ROX that can be detected by excitation at 576 nm and fluorescent emission at 597 nm
- Compatible with products for fragment analysis including Promega PowerPlex® 16 System
Details
Supporting Data
Figure 1 / 1
Click for expanded view
Ladder Properties:
• 22 discrete bands, ranging from 60 bp to 600 bp
Storage:
• Store at -20°C in small aliquots. For longer term storage, -70°C is recommended.
• The product is light-sensitive. Minimize light exposure.
D-Lactic Acid (D-Lactate) (Rapid) Assay Kit
K-DATE
SKU: 700004275
50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser)
| Content: | 50 assays (manual) / 500 assays (microplate) / 450 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Lactic Acid |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 0.5 to 30 μg of D-lactic acid per assay |
| Limit of Detection: | 0.21 mg/L |
| Reaction Time (min): | ~ 5 min |
| Application examples: | Wine, soft drinks, milk, dairy products (e.g. cream, milk / whey powder, cheese, condensed milk and yogurt), foods containing milk (e.g. dietetic foods, bakery products, baby food, chocolate, sweets and ice-cream), vinegar, fruit and vegetables, processed fruit and vegetables, meat products, food additives, paper (and cardboard), cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Methods based on this principle have been accepted by DIN, GOST, IDF, EEC, EN, ISO, OIV, IFU, AIJN and MEBAK |
The D-Lactic Acid (D-Lactate) (Rapid) test kit is suitable for the rapid, specific measurement and analysis of D-lactic acid in wine, beer, juice, milk, cheese, vinegar, meat and other food products.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
See our full list of lactic acid test kits and other organic acid assay kits.
Advantages
- Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
- Very rapid reaction with most samples (~ 5 min)
- Very competitive price (cost per test)
- All reagents stable for > 2 years after preparation
- Mega-Calc™ software tool is available from our website for hassle-free raw data processing
- Standard included
- Suitable for manual, microplate and auto-analyser formats
The D-Lactic Acid (D-Lactate) (Rapid) test kit is suitable for the rapid, specific measurement and analysis of D-lactic acid in wine, beer, juice, milk, cheese, vinegar, meat and other food products.
Amine-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt
Amine-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The amino group (NH2) is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. Reagent grade, for research use only.
Amine-Tri(3-methoxypropanamide-PEG10-Propargyl) Methane HCl salt is reactive with azide-bearing compounds or biomolecules via copper catalyzed azide-alkyne Click Chemistry to yield a stable triazole linkage. The amino group (NH2) is reactive with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde), etc. Reagent grade, for research use only.
BASIC AND ADVANCED LEAF CUTTING TOOL
Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.
About
BASIC LEAF CUTTING TOOL
The 3CR Bio Basic Leaf Cutting Tool is a lightweight, easy-to-use, low-cost punch designed for precise sampling of thin or soft materials, ideal for fieldwork. This tool is expertly designed to core, punch, and retrieve samples from thin or soft source materials such as leaves. The tool comprises a convenient, hollow plastic pen-style holder, mounted with a durable stainless steel cutting tip at one end. It features an internal push-button-operated plunger for safe and efficient sample disc ejection. The tool produces a precise ¼ inch diameter disc, making it ideal for detailed sampling tasks. For best results, clean the cutting tip between each sample extraction by coring blank filter paper or rinsing with water or ethanol. This low-cost leaf punch is lightweight, easy to use, and perfect for fieldwork.
ADVANCED LEAF CUTTING TOOL
The 3CR Bio Advanced Leaf Cutting Tool is designed to streamline and enhance your sampling process. This high-precision leaf tissue punch produces a single ¼ inch diameter disc effortlessly. It features a unique mechanism that securely holds a standard snap-top or screw-top 2 mL storage tube in a fixed position, allowing the sample to be received without any need for touching or manipulation. Ideal for fieldwork, this advanced leaf punch significantly reduces effort and time when performing multiple samplings, ensuring accuracy and efficiency in every use.
Efficient, leaf punch tools for quick, precise field sampling. For use with the 3CR Bio Leaf Sample Collection Kit.
D6352 MagPure Seed DNA Kit
Introduction
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Details
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from 50-100 mg easy-grinded plant (tender leaf) and seed |
| Applications | PCR, transgene detection, fluorescence quantitative PCR, southern blot, SNP site analysis, etc. |
| Purification technology | Magnetic beads technology |
| Process method | Manual or automatic |
| Sample type | Conventional economic plant samples |
| Sample amount | 50-100mg |
| Elution volume | ≥50μl |
| Time per run | ≤60 minutes |
Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.
Advantages
- Safe – require no phenol or chloroform extraction
- Fast – several samples can be extracted in 60 minutes
- High purity – high quality DNA, completely remove inhibitors
Kit Contents
| Contents | D635201 | D635202 | D635203 |
| Purification Times | 48 Preps | 96 Preps | 5 x 96 Preps |
| MagPure Particles | 1.7 ml | 4 ml | 18 ml |
| Buffer SOL | 40 ml | 90 ml | 500 ml |
| Buffer SDS | 4 ml | 9 ml | 50 ml |
| Buffer MPB | 40 ml | 70 ml | 350 ml |
| Buffer GW1 | 26 ml | 53 ml | 220 ml |
| Elution Buffer | 10 ml | 30 ml | 120 ml |
Storage and Stability
MagPure Particles should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
MagPure Seed DNA Kit supplies a simple and rapid extraction of genomic DNA from different plant pieces and seed. This kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation. The whole extraction only takes 60 minutes. This kit can be used on different automatic extraction machines like KingFisher ML, KingFisher Flex and KingFisher Duo. Purified DNA can be used directly for PCR, quantitative PCR, southern blot, hybridizationand transgenosis detection.
Our valued customer
Our Team
Our sales team is comprised of knowledgeable and experienced individuals in the field of science, who are committed to service, honesty, and responsibility. We strive to ensure that our customers receive unparalleled service that they won’t find anywhere else. We want our customers to feel confident that we will provide them with the very best service possible.
TANATHORN VITISANT
Sales manager
Phone : 081-875-1869
Email : [email protected]
Line id : @a3p-scientific
NUCHANAT JANPRAPAS
Area Sales Manager
Phone : 099-263-6624
Email : [email protected]
Line id : belongkong
NANTASAK SRISUWAN
Area Sales Manager
Phone : 094-562-5914
Email : [email protected]
Line id : north6906295
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