Overview

• Quantified standard to be used as a positive control or PCR quantification standard
• Vigorously quantified using multiple methods

Mastitis is the single most costly disease of dairy cattle resulting in the reduction of milk yield and quality. The inflammation of the utter is mainly caused by bacteria, and Staphylococcus aureus (S. aureus) is often considered the most common cause of contagious mastitis in dairy herds. S. aureus infection is estimated to be present in up to 90% of dairy farms and is responsible for 35% of the economic loss in the dairy industry. S. aureus is a facultatively anaerobic, Gram positive bacterium. The majority of S. aureus strains are catalase-positive and coagulase-positive, which forms the basis of traditional identification methodology.

Staphylococcus aureus Quantified Bacterial DNA Standard is prepared from cultured bacteria using Norgen’s sample preparation technology. The purified DNA is quantified vigorously using multiple methods including spectrophotometry, gel densitometry and real-time PCR. It is intended to be used as a positive control or PCR quantification standard for Staphylococcus aureus.

Details

Supporting Data

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Storage Conditions

Upon receipt, store Norgen’s Staphylococcus aureus Quantified Bacterial DNA Standard at -20^oC or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20^oC or lower.

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Nineteen discrete fragments ranging from 100 bp to 10000 bp
• Higher intensity reference bands at 500 bp, 1000 bp and 5000 bp

The Norgen HighRangerPlus 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our HighRangerPlus contains nineteen discrete fragments ranging from 100 bp to 10000 bp with higher intensity reference bands at 500 bp, 1000 bp and 5000 bp. This Ladder is ideal for size determination of digested DNA.

Contents:
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

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HighRanger Plus 100 bp DNA Ladder (Cat# 12000) – 100 loads

Ladder Properties:

• Nineteen discrete fragments ranging from 100 bp to 10000 bp
• Higher intensity bands at 500 bp, 1000 bp and 5000 bp for easy reference

Recommended Use:
Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 
This kit is stable for 2 years after the date of shipment.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Description 

The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Features

• Ready-to-use — Premixed with a loading buffer for direct loading, no need to boil.
• Two reference bands — 75 kDa (red) and 25 kDa (green)

Contents 

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate (pH 7.5 at 25°C), 2% SDS, 0.2 mM DTT, 3.6 M urea, and 15% (v/v) glycerol). 

Quality Control 

Under suggested conditions, PM2600 ExcelBand™ 3-color High Range Protein Marker resolves 12 major bands in 15% SDS-PAGE (Tris-Glycine buffer, MOPS, and MES buffer) and after Western blotting to nitrocellulose membrane. 

Storage

4°C for 3 months
-20°C for long term storage

The PM2600 ExcelBand™ 3-color High Range Protein Marker is a ready-to-use three-color protein standard with 12 pre-stained proteins covering a wide range of molecular weights from 10 to 245 kDa in Tris-Glycine Buffer (9 to 235 kDa in Bis-Tris (MOPS) buffer and 10 to 235 kDa in Bis-Tris (MES) buffer). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-PAGE (Tris-Glycine buffer). The PM2600 3-color High Range Protein Marker is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins.

Overview

• Detection kits for H5N1
• Available in TaqMan format for analysis

Influenza virus infection of birds, humans and other animals is a major public health problem worldwide. Influenza viruses are classified as either type A, B or C based on differences in their nucleoproteins and matrix proteins. The type A viruses are the most virulent human pathogens among the three influenza types and cause the most severe disease and epidemics. The different types can be further classified into subtypes based on antigenic differences in two surface glycoproteins; hemagglutinin and neuroamidase. All known subtypes of influenza A can be found in birds (H1-H16, N1-N9), while a limited number of the subtypes have been found in humans (H1-H3, N1 and N2). However, over the past few years, various subtypes of Influenza A viruses, including H5N1, have been reported to infect humans (WHO, 2006). In addition, the coexistence of human influenza viruses and avian influenza viruses may provide an opportunity for genetic material to be exchanged between these viruses. This could potentially create a new virulent influenza strain that is easily transmissible and lethal to humans (Food Safety Research Information Office, 2006). Thus, there is the need for sensitive diagnostic tests to allow for the rapid and early detection of these H5 influenza virus infections, to help reduce the risk of epidemics or pandemics in both animals and humans.

H5N1 TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

H5N1 TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

For research use only and NOT intended for in vitro diagnostics.

Details

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Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.

Introduction

Usages:

For the rapid detection and enumeration of coliform bacteria.

Principle:

Peptone and yeast extract powder provides carbon and nitrogen sources and trace elements; sodium chloride maintains osmotic equilibrium; agar as medium coagulant; dodecyl sulfate inhibit Gram-positive bacteria; chromogenic substrate and large intestine flora β- galactosidase-glucosidase specific reaction, hydrolysis of the substrate, the release of the color groups  produce green colonies on the light yellow plate.

Formulation (per liter):

Peptone :10g

Yeast extract powder: 3g

sodium chloride:5g

sodium lauryl sulfate:0.1g

Agar :12g

Chromogenic substrate 2.7g

Final pH 7.0 ± 0.2

How to use:

1. Weigh 32.8g of this product, adding 1L of distilled or deionized water , heated to boiling stirring until completely dissolved, dispensing into flask without autoclaving.

2,Take 25g or 25mL of sample with aseptic procedures, added to the flask containing 225mL of sterile phosphate buffered saline (or saline) is shaken thoroughly homogenized with a homogenizer or a 1:10 dilution 1min, then 1:10 dilution continue to select the appropriate serial dilutions of three, two plates each dilution was inoculated.

3, the use of pour method: The medium is cooled in a water bath to about 50 , sterilized petri dish having a diameter 90mm, 1ml samples were inoculated per dish, then poured into about 15ml of the above dissolution medium, and mix to solidify, upside down, 37 for 24 hours.

4, using surface inoculation: cooled to about 50 , shake devoted to the medium in sterile petri dish. Can be stored in the refrigerator for a day or stored at room temperature for several days (dark, 4 ). The sample was streaked method or filter method, 37 for 24 hours.

5, observe the results.

Quality control:

This product appear light yellow after pouring into plate, these strains were inoculated after 36 ± 1 18 ~ 24h culture growth in the following table.

Growth of bacteria were cultured bacteria numbers feature

Escherichia coli ATCC25922 good green colonies

Citrobacter ATCC8090 good green colonies

Salmonella typhimurium CMCC50115 good colorless colonies

Enterococcus faecalis ATCC29212 suppressed —–

Storage: Store at 10-30 , dark, cool and dry place, tighten the cap immediately after use. Storage period of two years.

1000mL

The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.

The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.

The Opentrons HEPA Module enables you to run sensitive contamination-prone applications. It removes 99.99% of 0.3 μm DNA-containing particulates and biological contaminants like bacteria, fungi, and other microorganisms from the air, creating a clean work environment inside the OT-2.

The Opentrons HEPA Module comes in two different voltages. Your account manager will help you choose the correct one for your geographic region.