Description

Specifications

Cluster of differentiation 5 (CD5) is expressed in high levels on the surface of T cells, while controversy surrounds the expression levels and role of CD5 in B cells. As a part of a diagnostic panel, its utility lies predominantly as a marker for T cells, with over 70% of T cell neoplasms expressing CD5. In particular, it is correlated with chronic lymphocytic leukemia/small lymphocytic lymphomas, mantle cell lymphoma, as well as a subset of diffuse large B cell lymphomas. CD5 demonstrates positive expression in thymic carcinomas, and is not as sensitive as CD3. CD5 also has value as a prognostic indicator, being associated with poor prognosis in acute T cell lymphoblastic leukemia.

K-BETA3

SKU: 700004268

100 assays per kit

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

See more of our amylase assay kits and other kit products for measurement of enzyme activities.

Advantages

• Very cost effective 
• All reagents stable for > 2 years as supplied 
• Only enzymatic kit available 
• Very specific 
• Simple format 
• Rapid reaction 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included

The Betamyl-3; β-Amylase test kit is suitable for the specific measurement and analysis of β-amylase in malt flour.

See more of our amylase assay kits and other kit products for measurement of enzyme activities

Techinical parameter

Model:
SH7006,SH9006,SH9009

Brand:
Yingtai

Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Methyltetrazine-PEG7-DBCO is a PEG linker with a terminal TCO reactive reagent and a DBCO group. DBCO is commonly used for copper-free Click Chemistry reactions. Methyltetrazine can be used to convert azido-containing peptides or proteins into tetrazine-modified peptides or protein without catalyst or axillary reagents. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-TDFR-200A

SKU: 700004347

The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.

View Dietary Fiber Measurement Guide – Which Method for which sample?

Dietary fiber can generally be described as the carbohydrate content of food that is not digested in the human small intestine. It passes into the large intestine where it is partially or fully fermented. These characteristics of dietary fiber are associated with its numerous well documented health benefits.

Dietary Fiber is a mixture of complex organic substances, including hydrophilic compounds, such as soluble and insoluble polysaccharides and non-digestable oligosaccharides, as well as a range of non-swellable, more or less hydrophobic, compounds such as cutins, suberins and lignins. The procedures for the determination and analysis of total dietary fiber as outlined in our assay protocol are based on the methods of Lee et al.¹ and Prosky et al.^2,3 (AOAC 991.43, AOAC 985.29, AACC 32-07.01 and AACC 32-05.01). However, the enzymes in the Megazyme Total Dietary Fiber Kit can also be used in other dietary fiber analytical methods such as AACC Method 32-21.01 and AACC Method 32-06.01.

1. Association of Official Analytical Chemists. (1985). Official Methods of Analysis, 14th ed., 1st suppl. Secs. 43, A14-43, A20, p.399.
2. Association of Official Analytical Chemists. (1986). Changes in methods. J. Assoc. Off. Anal. Chem., 69, 370.
3. Association of Official Analytical Chemists. (1987). Changes in methods. J. Assoc. Off. Anal. Chem., 70, 393.

See General Referee Reports: Journal of AOAC INTERNATIONAL, Vol. 81, No. 1, 1998. 

Two separate methods are described in the associated assay protocol:

METHOD 1: DETERMINATION OF TOTAL, SOLUBLE AND INSOLUBLE DIETARY FIBER
Based on AOAC Method 991.43 “Total, Soluble, and Insoluble Dietary Fiber in Foods” (First Action 1991) and AACC Method 32-07.01 “Determination of Soluble, Insoluble, and Total Dietary Fiber in Foods and Food Products” (Final Approval 10-16-91).

METHOD 2: DETERMINATION OF TOTAL DIETARY FIBER
Based on AACC method 32-05.01 and AOAC Method 985.29.

Note that a letter of endorsement from the original method developer, Dr. Leon Prosky, is included in the Documents Tab.

See our full range of starch and dietary fiber products.

Validation of Methods

Advantages

• Very competitive price (cost per test)   
• All reagents stable for > 2 years 
• High purity / standardised enzymes employed 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Simple format

The Total Dietary Fiber Assay Kit for the analysis of Total, Soluble and Insoluble Dietary Fiber according to AOAC and AACC approved methods.

PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose.

PEG3-bis(Amino-Tri-(Propargyl-PEG2-ethoxymethyl)-methane) is a crosslinker consisting of six propargyl groups. The propargyl groups can form triazole linkage with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry. The hydrophilic PEG spacer increases solubility in aqueous media. Reagent grade, for research purpose.