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A3P SCIENTIFIC
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A3P (ATP) acts as a powerhouse of energy, driving transformation and innovation, enabling new and better possibilities for growth and advancement.
Authorized distributor
3CR Bioscience Limited’s patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent results.
Plasma/Serum Circulating DNA Purification Kits (Slurry Format)
Overview
- Isolate all sizes of circulating DNA from plasma and serum samples
- Isolate Viral and Bacterial DNA
- Versatile plasma and serum input volumes
- Concentrate circulating DNA into small elution volumes
- Different elution strategies depending on the downstream application
- Isolate inhibitor-free circulating DNA for any application including PCR, qPCR, methylation-sensitive PCR
- Compatible with Streck Cell-Free DNA BCT® Tubes
- Isolate DNA with high quality and quantity from milk
- Purification is based on Norgen’s proprietary Silicon Carbide resin matrix
Norgen’s Plasma/Serum Circulating DNA Purification Kits (Slurry Format) provide efficient methods for the purification of fragmented free-circulating DNA from human plasma or serum. The kit is able to isolate all sizes of circulating DNA. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating DNA from large sample volumes. DNA can be isolated from either fresh or frozen samples using this kit. The kit will also isolate viral and bacterial DNA from plasma/serum. Typical yields of purified free-circulating DNA will vary depending on the input sample (1-100ng/mL circulating DNA in human plasma), with more concentrated samples tending to yield more free-circulating nucleic acids. The purified, inhibitor-free plasma/serum free-circulating DNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive PCR and Southern Blot analysis.
Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 50 μL to 400 μL. Preparation time for a single sample is less than 30 minutes.
Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Midi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 400 μL to 2 mL. Preparation time for a single sample is less than 45 minutes.
Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format)
Norgen’s Plasma/Serum Circulating DNA Purification Maxi Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating DNA from various amounts of plasma/serum ranging from 2 mL to 10 mL. Preparation time for a single sample is less than 45 minutes.
Details
Supporting Data
Figure 1 / 10
Click for expanded view
| Kit Specifications | |
| Minimum Plasma/Serum Input | 50 μL |
| Maximum Plasma/Serum Input | 400 μL |
| Minimum Elution Volume | 100 μL |
| Time to Complete Purifications | < 30 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
| Component | Cat. 50600 (50 preps) | Cat. 51200 (20 preps) | Cat. 51300 (10 preps) |
|---|---|---|---|
| Lysis Buffer D | 65 mL | 125 mL | 3 x 125 mL |
| Slurry A1 | – | 6 mL | – |
| Slurry A2 | 12 mL | – | 12 mL |
| Binding Buffer B | 20 mL | 6 mL | 20 mL |
| Binding Buffer C | – | – | 30 mL |
| Wash Solution A | 2 x 38 mL | 2 x 38 mL | 3 x 38 mL |
| Elution Buffer B | 8 mL | 8 mL | 15 mL |
| Mini Filter Spin Columns | 50 | 20 | – |
| Midi Filter Spin Columns | – | – | 10 |
| Midi Collection and Elution Tubes | – | – | 20 |
| Collection Tubes | 50 | 40 | 10 |
| Spin Columns | – | 20 | 10 |
| Elution Tubes (1.7 mL) | 50 | 40 | 10 |
| Product Insert | 1 | 1 | 1 |
Saxitoxin (PSP) Lateral Flow Kit (Freshwater Streams and Source Water)
- Screening of saxitoxin in Freshwater Streams and Source Water samples as low as 70 ppt
- Format: 10 tests (5 tests/5 control)
- Water Sample Bottles provided
- Run Time: 15 Minutes
Description
Saxitoxins (STXs) are naturally occurring alkaloids produced by some marine dinoflagellates and by strains of various species of freshwater cyanobacteria. Saxitoxin is one of the prevalent paralytic shellfish toxins (PSTs). It belongs to a family of potent neurotoxins with a molecular weight around 300 Da. Saxitoxin and its derivatives are alkaloids composed of a tetrahydropurine ring system with a highly polar guanidinium group. Due to their significant toxicity, saxitoxins are closely monitored in marine environments where they can accumulate during harmful algal blooms (HABs). In 2009 an action level of 0.6ppb (600ppt) was recommended by EFSA as a guidance framework have been established to manage saxitoxin levels in water.
Screening of saxitoxin in Freshwater Streams and Source Water samples as low as 70 ppt
Format: 10 tests (5 tests/5 control)
Water Sample Bottles provided
Run Time: 15 Minutes
Cell Culture Plate 48 Wells
Cell Culture Plate 48 Wells
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells, 12wells, 24wells., 48wells, 96wells
PRODUCT FEATURES
- The product is made of medical grade USP CLASS VI polymer polystyrene
- The product is made under a 100,00- class dust-free manufacturing site
- Two kinds of product line up are providing.
For adherent cell culture: Initial adherence and proliferative property of cells via hydrophilic surface treatment.
For suspension cell culture: The surface is resistant to cell adherence, which minimizes damage or loss of cell.
- Gamma radiation sterilization
- Non-Pyrogenic, DNase/Rnase free.
Cell Culture Plate provide excellent smoothness and uniformity based on accurate molding technology. By such property, clear view can be available when examine with a microscope.
Specifications: 6wells, 12wells, 24wells., 48wells, 96wells
PCR Decontamination Kit
OverView
The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.
- The double-strand specific property of the dsDNase allows decontamination with primers and probe present.
- Efficient for end-point PCR, probe-based qPCR, and some SYBR based qPCR mixes.
- Contaminating bacterial DNA can be reduced to levels below the detection limit.
- Fast and easy protocol.
- Flat NTCs (No Template Controls).
Decontamination of master mixes without reduction of sensitivity has always been a challenge. Especially when minor amounts of DNA are targeted, contaminating DNA is a major problem. Any loss of sensitivity in the qPCR assay caused by the decontamination protocol is unacceptable.
In Figure 1, it is demonstrated that the PCR decontamination kit can remove contaminating DNA from a qPCR mix to non-detectable levels (flat NTC), without affecting the sensitivity of the qPCR.
Kit Contents
- DTT (Inactivation Aid)
- dsDNase
The PCR Decontamination Kit can remove contaminating DNA in PCR master mixes, without reduction of PCR sensitivity.
DBCO-amine
DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
DBCO-amine is a simple building block containing a DBCO moiety and will add minimal spacer to the modified molecules. In the presence of activators such as EDC or HATU, this reagent can be used to derivatize carboxyl groups or activated esters (e.g. The NHS ester) through a stable amide bond. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Our valued customer
Our Team
Our sales team is comprised of knowledgeable and experienced individuals in the field of science, who are committed to service, honesty, and responsibility. We strive to ensure that our customers receive unparalleled service that they won’t find anywhere else. We want our customers to feel confident that we will provide them with the very best service possible.
TANATHORN VITISANT
Sales manager
Phone : 081-875-1869
Email : [email protected]
Line id : @a3p-scientific
NUCHANAT JANPRAPAS
Area Sales Manager
Phone : 099-263-6624
Email : [email protected]
Line id : belongkong
NANTASAK SRISUWAN
Area Sales Manager
Phone : 094-562-5914
Email : [email protected]
Line id : north6906295
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