Biotin-PEG2-C4-alkyne is biotinylation reagent that can react with azide moiety in Cu(I)-catalyzed Click Chemistry reaction to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Biotin-PEG2-C4-alkyne is biotinylation reagent that can react with azide moiety in Cu(I)-catalyzed Click Chemistry reaction to form a stable triazole linkage. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Magnetic Beads (microRNA & Oligo Purification)

Solid Phase Reversible Immobilization magnetic beads are often used for DNA purification because they are simple, fast, and effective. The beads are paramagnetic particles coated with carboxyl groups that reversibly bind to nucleic acid. However, Standard SPRI beads can only purify DNA/RNA fragments that are 100 base pairs or longer. DNA/RNA fragments shorter than 100 base pairs are not effectively recovered by SPRI beads. We have developed Magnetic Beads (microRNA & Oligo Purification) to solve the problem.

With our proprietary beads technology, the beads overcomes the hurdle of the short DNA/RNA recovery problem. The magnetic beads is ideal for microRNA purification, oligo purification, short DNA/RNA purification, and removing impurities and unwanted components such as dNTPs, detergents, salts, proteins, and other contaminants effectively. The magnetic bead reagents are RNase-free and can be used for both DNA and RNA applications.

Our magnetic beads are optimized for microRNA purification, oligo purification, and DNA/RNA purification. The fragments can be as short as 20 bases, such as microRNA, tRNA, dsDNA fragments 20 bp or longer, ssDNA fragments 20 nt or longer, RNA fragments 20 nt or longer, DNA/RNA hybrid fragments 20 bp or longer, and oligos and chimeric oligos 20 nt or longer. Purified short DNA and RNA fragments are ideal for applications requiring high-quality fragments, as the fragments are free of impurities and contaminants.

Comparison of short DNA fragments recovery. BioDynami 20 bp DNA ladder (Cat. # 10002) was used as DNA input. Ampure XP beads, BioDynami beads (DNA & RNA purification) and BioDynami beads (microRNA & Oligo purification) were tested. Only BioDynami beads (microRNA & Oligo purification) successfully recovered DNA fragments between 20-100 bp.

Recovery of DNA and RNA oligos with BioDynami magnetic Beads (microRNA & Oligo Purification). 20 nt of DNA oligos and RNA oligos were used as input. Input and recovered oligos were quantified with BioDynami ssDNA Quantification kit (Cat. # 40043) and BioDynami RNA Quantification kit (Cat. # 40044).

Features

• Effective purification of short DNA and RNA samples
  • • microRNA
    • tRNA
    • dsDNA fragments 20 bp or longer
    • ssDNA fragments 20 nt or longer
    • RNA fragments 20 nt or longer
    • DNA/RNA hybrid fragments 20 bp or longer
    • Oligo and chimeric oligo 20 nt or longer
• Removal of impurities and unwanted reaction components
• Compatibility: Works with both manual and automated procedures

Introduction

029070 Nitrate Reduction Reagent

Usage:For Nitrate Reduction Test.

Specification:10ml*2vials

10ml*2vials

Introduction

The HiPure Plasmid DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.

Details

Specifications

Principle

The HiPure Plasmid procedure is based on alkaline lysis of bacterial cells followed by adsorption of DNA onto silica in the presence of high salt. The unique silica membrane used in the kit completely replaces glass or silica slurries for plasmid DNA minipreps. The procedure consists of 3 basic steps: Preparation and clearing of a bacterial lysate by alkaline method，then transfer the supernatant to column to bind DNA. After washing proteins and other impurities, nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High purity – purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast – silica gel column purification is much faster than ion exchange
• High yield – up to 10mg plasmid can be binded in one column
• Low endotoxin – the obtainedplasmid can be directly used for cell transfection and animal injection, etc.

Kit Contents

Storage and Stability

The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37℃ to dissolve.

Purchase Guide

For any technical problems or customized products, please contact us.

F&Q about Endotoxin-free Plasmid Extraction Kit — P1156 ←click here

The HiPure Plasmid DNA Mega Kits combine the power of HiPure technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high-quality DNA. HiPure DNA columns facilitate the binding, washing and elution steps thus enabling multiple samples to be simultaneously processed. Plasmid DNA purified by this system is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations. Up to 10 mg high copy number plasmid DNA can be purified from 500 mL overnight culture.

Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG5-NHS ester has a propargyl group and an NHS ester group. The terminal NHS ester and is an amine reactive moeity which is useful for derivatizing peptides, antibodies, amine coated surfaces etc. The propargyl group can participate in Click Chemistry reactions with azide compounds, copper is needed for catalyzation. The PEG units can enhance the hydrophilicity of the molecule. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.

Biotin-PEG8-alkyne is a PEG linker containing a biotin group and a terminal alkyne. Biotin is useful for affinity-based applications such as pull-down assays while terminal alkynes are used in copper (I) click chemistry with azide groups on a target molecule. The inclusion of a PEG linker in this molecule improves its solubility in water.