Description

The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl₂, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.

Features

• 5’→3’ DNA polymerase activity
• No detectable 3’→5′ exonuclease (proofreading) activity
• Generates PCR products with 3′-dA overhangs
• High yield PCR 
• High reproducibility
• Reduced  pipetting errors
• Includes tracking dye for direct loading after PCR

Applications 

• Routine PCR
• Colony PCR
• High throughput PCR
• Amplification of DNA fragments up to 8 kb
• Generation of PCR products for TA cloning
• DNA labeling

Storage

4°C for 6 months
-20°C for 24 months

The ExcelTaq™ 5X PCR Master Dye Mix is a ready-to-use mixture for amplifying targeted DNA fragments. It is designed to serve as ready-to-use master mix for virtually all PCR applications. The mixture contains all components for PCR with the exception of template and primers. This not only saves valuable time in the laboratory, but also reduces the number of pipetting and reagent handling errors. The PCR Master Dye Mix is supplied as a 5X concentrated ready-to-use mix, that is a mixture of recombinant Taq DNA polymerase, reaction buffer, MgCl2, dNTP, enzyme stabilizer and PCR-friendly loading dye solution containing tracking dye (Bromophenol blue) enabling efficient amplification of template in PCR and allows the user to prepare a PCR reagent – loading dye master mix conveniently.

DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.

Perfect for:

• End-point gel electrophoresis DNA detection
• down-stream applications (e.g. sub-cloning)
• facilitating use of different RPA reaction volumes, or variation of component ratios.

Product code: TALQBAS01

DNA amplification in a flexible liquid format. Contains all the enzymes and reagents necessary for the amplification of DNA, the user need only supply primers, dNTPs and template. See manual for more information. Click to order oligonucleotides.

Sulfo DBCO-Maleimide a water-soluble sulfhydryl reactive containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Sulfo DBCO-Maleimide a water-soluble sulfhydryl reactive containing a maleimide group and a DBCO moiety. Maleimide group specifically and efficiently reacts with thiols to form stable thioether bonds. The low mass weight will add minimal spacer to modified molecules and will enable simple and efficient incorporation of DBCO moiety onto cysteine-containing peptides or other thiol-containing biomolecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

K-CITR

SKU: 700004274

72 assays (manual) / 720 assays (microplate) / 840 assays (auto-analyser)

The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.

Note for Content: The number of manual tests per kit can be doubled if all volumes are halved.  This can be readily accommodated using the MegaQuant^TM  Wave Spectrophotometer (D-MQWAVE).

View our full list of organic acid test kits.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• Reconstituted citrate lyase stable for 4 weeks at 4^oC / 6 months below -10^oC 
• Buffer / cofactor / enzyme tablets for efficient use of kit components 
• PVP incorporated to prevent tannin inhibition 
• Very competitive price (cost per test) 
• Mega-Calc™ software tool is available from our website for hassle-free raw data processing 
• Standard included
• Suitable for manual, microplate and auto-analyser formats

The Citric Acid test kit is a flexible and simple method for the rapid and reliable measurement and analysis of citric acid (citrate) in foods, beverages and other materials.

Overview

• ASTM Type I Water
• Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
• Nuclease-free, protease-free, endotoxin-free
• Suitable for molecular biology applications (qPCR, NGS etc.)
• Suitable for cell culture applications
• Produced in an ISO certified GMP laboratory
• Water system is routinely monitored for compliance with QC standards

The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.

40 Layer Cell Factory Double Wide Mouth

Huayi Cell Factory is a robust and easy-to-use cell

culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.

PRODUCT FEATURES

• The product is made of medical grade USP CLASS VI polymer polystyrene
• The product is made under a 100,00- class dust-free manufacturing site
• Surface TC treatment ensures efficient cell attachment
• The product assembled with ultrasonic welded technology
• Versatile port design facilitates both pouring and aseptic filling techniques
• Gamma radiation sterilization
• the cell culture surface area

of one 10-layer Cell Factory unit is equivalent to the area

of 36 T-175 flasks

Huayi Cell Factory is a robust and easy-to-use cell

culture platform for applications in the production of human and animal vaccines, therapeutic proteins, cell therapy, and gene therapy.

To address the needs of your workflow, We have three kinds of mouth, which is available on most cell culture products to ensure consistent performance from lot to lot and from format to format.

Specifications: 1 layer 2 layers, 5 layers, 10 layers and 40 layers.