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A3P (ATP) acts as a powerhouse of energy, driving transformation and innovation, enabling new and better possibilities for growth and advancement.
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3CR Bioscience Limited’s patented genotyping chemistry, PACE, is the latest in allele-specific chemistry. It provides consistent results.
Bis-PEG11-DBCO
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Bis-PEG11-DBCO represents a homobifunctional PEG linker with DBCO moieties on either side that perform copper-free catalyzed click chemistry with azides. The PEG spacer imparts water-solubility and helps with fine-tuning DMPK properties. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms)
The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.
NGS FFPE DNA Library Prep Kit Workflow
FFPE samples are a great resource for biomedical research. However, the methods for fixation and condition of storage significantly damage the DNA in the samples. Thus, the extraction of high quality DNA from FFPE samples is often a challenge. Low yield and low quality of FFPE DNA usually are common because of the limited tissue material and the DNA degradation.
As a result, it is usually difficult to construct high quality NGS libraries from low amount and low quality of FFPE DNA. In order to address this issue, we have developed the NGS FFPE DNA Library Prep Kit to make high quality libraries from the low input of FFPE DNA samples.
Three index types are available for the NGS FFPE DNA Library Prep Kit of the illumina platform:
Non-index (Cat.# 30035): Libraries do not have index.
Index (Cat.# 30037): Each of our index primers contains a unique barcode DNA (6 bases long) that can be used to identify individual library. Multiplexing of libraries is up to 48 samples. Index information can be downloaded here.
Unique dual index (Cat.# 30039): FFPE DNA library multiplexing is possible with 96 samples based on the unique dual indexing system. Our unique Four–Base Difference Index System allows us to make indexes that have at least 4 bases different from each other in the 8-base index sequence. Our unique dual indexing primers can effectively remove NGS errors including index hopping, de-multiplexing errors, index cross-contamination, mis-assignments etc. The unique dual index primer set includes 96 pre-mixed unique pairs of i5 and i7 index primers. Index information can be downloaded here.
Indexes are available for the MGI platform kits (Cat.# 34037).
Kit advantages:
- Fast and simple protocol
- Making libraries in just 1.5 hours
- 10 minutes of hands-on time
- Easy procedure
- Ready-to-use master mix to reduce the tedious mixing
- Less reaction components to simplify the reaction setup
- Less magnetic beads needed for the purification steps: saving more than 50% of the expensive beads
- Low input FFPE DNA: From 10 ng to 400 ng
Comparison of library conversion efficiency under the same condition. Input DNA amount is 25 ng.
Comparison of library yield under the same condition. Input DNA amount is 25 ng.
The NGS FFPE DNA Library Prep Kit (illumina and MGI Platforms) was developed for the construction of high quality FFPE DNA libraries using 10 ng to 400 ng of input DNA isolated from formalin-fixed, paraffin-embedded (FFPE) samples. The DNA damage caused by fixation makes it difficult to construct high quality libraries. Our kit has been optimized to repair damaged DNA in the reactions. Multiplexing of the NGS FFPE DNA Library is possible.
tert-Butyl (2-(prop-2-yn-1-yloxy)ethyl)carbamate
tert-Butyl (2-(prop-2-yn-1-yloxy)ethyl)carbamate is crosslinker consisting of a propargyl group and a t-Boc protected amine group. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The t-Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research use only.
tert-Butyl (2-(prop-2-yn-1-yloxy)ethyl)carbamate is crosslinker consisting of a propargyl group and a t-Boc protected amine group. The propargyl group reacts with azide-bearing compounds or biomolecules via copper catalyzed Click Chemistry reactions. The t-Boc protected amine can be deprotected under mild acidic conditions. Reagent grade, for research use only.
TruScript Reverse Transcriptase and Kits
Overview
- Convenient – With the ready-to-use Master Mix, the user needs only to add template to the master mix and enzyme in order to set up the reverse transcriptase reaction
- Time Savings – Set up RT reactions in a shorter time since less pipetting steps are required
- Cost Efficient – No need to buy separate enzymes, dNTPs and buffers. All are included with the ready-to-use Master Mix kits
- High Sensitivity and Yield – the optimized Master Mix allows for highly sensitive amplifications with high yields of PCR products
- Robust Enzyme – broad range of working temperatures from 37°C to 60°C. Capable of amplifying difficult templates with a high degree of reproducibility
TruScript Reverse Transcriptase is Available in Three Convenient Formats:
1. TruScript Reverse Transcriptase Kit (Cat.#54440)
This kit contains 5X RT Buffer and a vial of TruScript Enzyme Mix (200 units/µL). This enzyme can be used for reverse-transcription reactions with any user-supplied primers.
2. TruScript First Strand cDNA Synthesis Kit (Cat.#54420)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of total RNA (both poly A- or non-poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and primers (oligo dT and random hexamers) for effective cDNA synthesis from total RNA transcripts.
3. TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.#54400)
This is an all-in-one, ready-to-use product for simple set-up of reverse transcription of messenger RNA (poly A-containing transcripts).
The kit contains the 2x Reaction Mix and the TruScript Enzyme Mix. The 2x Reaction Mix contains a blend of buffer, nucleotides and oligo dT primer for the effective cDNA synthesis from total RNA transcripts or enriched mRNA sample.
Which TruScript Kit is Best for Your Needs?
| TruScript Reverse Transcriptase | TruScript First Strand cDNA Synthesis Kit for mRNA | TruScript First Strand cDNA Synthesis Kit | |
| Kit contains only reverse transcriptase enzyme and buffer (no primers or other buffer components) | |||
| Your template is enriched mRNA | |||
| Your template is total RNA (poly A OR non-poly A-containing transcripts) | |||
| Kit contains oligo dT | |||
| Kit contains both oligo-dT primer and Random Hexamers | |||
| Kit contains reaction buffer with nucleotides and primer | with oligo-dT | with oligo-dT and random hexamers | |
| You have your own first strand synthesis primer | |||
| Your template is microRNA (using your own primers) | |||
| Cat.#54440 | Cat.#54400 | Cat.#54420 |
Details
Supporting Data
Figure 1 / 2
Click for expanded view
TruScript First Strand cDNA Synthesis Kit for mRNA (Cat.# 54400) – Kit Components
1. TruScript Enzyme Mix
2. 2x Reaction Mix
3. Nuclease-Free Water
4. Product Insert
Storage Conditions and Product Stability
Norgen’s TruScript Reverse Transcriptase and the 5x RT Buffer should be stored at -20°C. These reagents should remain stable for at least 1 year in their unopened containers.
| Component | Cat. 54440 (10,000 units) | Cat. 54420 (50 rxns) | Cat. 54410 (50 rxns) |
|---|---|---|---|
| TruScript™ Reverse Transcriptase (200 units/ μL) | 50 μL | – | – |
| 5x RT Buffer | 1 mL | – | – |
| TruScript™ Enzyme Mix | – | 100 μL | 100 μL |
| 2x Reaction Mix | – | 500 μL | – |
| 2x Reaction Mix for mRNA | – | – | 500 μL |
| Nuclease-Free Water | 1.25 mL | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 | 1 |
CDL7M Largest Capacity Refrigerated Centrifuge
Application
Hereby we (Changsha Yingtai Instrument Co.,Ltd) confirm that CDL7M centrifuge is recommended for Graphene Base Manufacturing Industries. This centrifuge type is widely used in Large Laboratory Systems, Graphene Base Manufacturing Industries, and also used in the field of blood stations, pharmaceutical factories, biochemistry, biological products, and so on
Features
1. Widely used in the field of blood bank, pharmaceutical factory and laboratory.
2. Brushless frequency motor, in great torque, free maintenance, no powder pollution, quick in speed up and down.
3. Digital display which indicates the program, speed, time,RCF, temperature.
4. Micro computer control, there are 10 kinds of program and 10 kinds of acceleration and deceleration for your choice.
5. There are 3 kinds of rotors for your choice.
6. Electric lid lock, compact design, super speed and imbalance protection.
7. The centrifuge body is made of high-quality steel, safe and reliable.
CDL7M Technical Parameter：
| Max. Speed | 8000rpm |
| Max. RCF | 14551×g |
| Max. Capacity | 6×2400ml |
| Time Range | 1~9h59min |
| RPM/RCF Convert | Yes |
| Noise (dB) | ≤ 65 |
| Temperature | -20~40℃ |
| Acc/Dec | 10 Kinds |
| Speed Accuracy | ±20r/min |
| Temperature Accuracy | ±1℃ |
| Voltage(V/Hz) | AC 220V/380V 50HZ/60HZ |
| Size (W x D x Hmm) | 940×890×1000mm |
| Net Weight(Kg) | 570KG |
| Certificates | CE,ISO & Calibration report are available |
Matched Rotors for CDL7M
| Order No. | Rotor No. | Max Speed (rpm) | Max Volume(ml) | Max.RCF（×g） |
| 7M-1 | Swing Rotor | 4000 | 6×2400ml | 5330 |
| 7M-2 | Angle Rotor | 8000 | 6×1000ml | 14551 |
| 7M-3 | Swing Rotor | 4200 | 2×6×1000ml | 5680 |
Respiratory Syncytial Virus A (RSV-A) TaqMan RT-PCR Detection Kits
Overview
- Detection kits for RSV-A
- Available in TaqMan format for analysis
Norgen’s Respiratory Syncytial Virus A (RSV-A) TaqMan RT-PCR Detection Kit is designed for the detection of RSV-A specific RNA in a real-time PCR based on the use of TaqMan technology. This kit is designed for research use only and not for use in diagnostic procedures. The detection of RSV-A specific RNA is based on TaqMan one-step RT-PCR providing a simple, reliable and rapid result for the detection of RSV-A infection. Norgen’s TaqMan RT-PCR Kit includes a PCR control to monitor for PCR inhibition, and to validate the quality of the sample and the detection result. The RSV-A TaqMan RT-PCR Kit comprises Master Mix for the target and PCR control detection, Primer & Probe Mix, as well as a positive control and a negative control (nuclease-free water) to confirm the integrity of the kit reagents.
RSV-A TaqMan RT-PCR Kit, 100 reactions
- Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
- Specific Primer and Probe mix for the pathogen/virus/viroid of interest
- Primer and Probe mix
- Positive and negative control to confirm the integrity of the kit reagents
RSV-A TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions
- Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
- Nuclease-free water
- Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix
For research use only and NOT intended for in vitro diagnostics.
Details
Supporting Data
Figure 1 / 3
Click for expanded view
Storage Conditions and Product Stability
All kit components can be stored for 1 year after the date of production without showing any reduction in performance.
All kit components should be stored at -20°C upon arrival. Repeated thawing and freezing (> 2 x) of the Master Mix and Positive Control should be avoided, as this may affect the performance of the assay. If the reagents are to be used only intermittently, they should be frozen in aliquots.
| Component | Cat. TM34250 (100 preps) | Cat. TM34210 (100 preps) |
|---|---|---|
| MDx TaqMan 2X PCR Master Mix | 2 x 700 μL | – |
| RSV-A Primer & Probe Mix | 280 μL | 280 μL |
| RSV-A Positive Control | 150 μL | 150 μL |
| Nuclease-Free Water (Negative Control) | 1.25 mL | 1.25 mL |
| Product Insert | 1 | 1 |
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Our Team
Our sales team is comprised of knowledgeable and experienced individuals in the field of science, who are committed to service, honesty, and responsibility. We strive to ensure that our customers receive unparalleled service that they won’t find anywhere else. We want our customers to feel confident that we will provide them with the very best service possible.
TANATHORN VITISANT
Sales manager
Phone : 081-875-1869
Email : [email protected]
Line id : @a3p-scientific
NUCHANAT JANPRAPAS
Area Sales Manager
Phone : 099-263-6624
Email : [email protected]
Line id : belongkong
NANTASAK SRISUWAN
Area Sales Manager
Phone : 094-562-5914
Email : [email protected]
Line id : north6906295
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