Overview

• Convenient
• One cDNA Synthesis, Multiple microRNAs and microRNA-targets analyzed
• Time Savings
• Cost Efficient
• High Sensitivity and Yield
• Robust Enzyme
• Available in 12 or 50 reaction size

Norgen’s microScript microRNA cDNA Synthesis Kit is an all-in-one, ready-to-use product for the reverse transcription of microRNA from either Total RNA preparations or enriched microRNA preparations. The kit contains the 2x Reaction Mix and the microScript microRNA Enzyme Mix. The kit utilizes Norgen’s microScript Reverse Transcriptase, a mutant version of Moloney Murine Leukemia Virus (M-MuLV) Reverse Transcriptase. It has reduced RNase H activity and increased thermal stability.

The workflow of Norgen’s microScript microRNA cDNA Synthesis Kit involves a simple, single-tube set-up by the mixing of 2x Reaction Mix, Enzyme Mix and the RNA template. The reaction can then be carried out in a thermocycler. A poly (A) tail is first added to the RNA template, followed by cDNA synthesis using an adapter primer. In addition to the ease-of-use, the single-tube set-up provides superb consistency and sensitivity. The cDNA could be used in a PCR or qPCR amplification using a Universal PCR Reverse Primer and the forward primer that contains the sequence of the microRNA of interest. A single cDNA preparation could be used for PCR amplification of a number of different microRNAs. In addition, the cDNA preparation could be used for PCR or qPCR detection (using gene-specific forward and reverse primers) of mRNA or large RNA if total RNA preparation was the starting template. This could allow for parallel evaluation of expression level of microRNAs and microRNA-targets.

Details

Supporting Data

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Storage Conditions and Product Stability
This kit is stable for 1 year after the date of shipment .

Character

High flatness sample shelf:

Silicone oil thermal conductive shelf, high-precision processing method of upper and lower clamping, shelf temperature uniformity can reach ±0.5°c

Integrated cavity:

Integrated chamber, the shelf and cold trap are located in the same chamber, the steam conduction is faster and the freeze-drying effect is better

Refrigerating and heating control system:

Using thermally conductive silicone oil with a high temperature range and precise PID control algorithm, the control accuracy can reach ±0.5°C. Sample shelf temperature range: -70°C~ +80°C

Freeze-drying endpoint testing system:

The pressure comparison method is used to determine the freeze-drying end point. The freeze-drying end point test can be automatically performed after the analytical drying stage to ensure that the moisture content of the material reaches the standard requirements.

Vacuum precise control and adjustment system:

The sublimation and analytical drying processes are controlled and adjusted with high precision to make the freeze-drying process warmer and the sample sublimation more reliable.

Pulse backfill system:

Three backfilling modes can be selected: slow, medium and fast, which avoids the problem of effective substances being blown away and lost due to the aeration of granular and flocculent materials after freeze-drying.

Manual automatic mode selection:

Manual mode is used to explore process parameters (strong human intervention), automatic mode is used in the mature stage of the process, one-click operation, simple and convenient

Data recording and analysis system:

Through the PC host computer, all operating parameters and background parameters of the equipment can be recorded in real time, and all parameter action changes and action changes of operating components can be recorded. 

Freeze-drying process recipe storage function:

The host can store 60 sets of fixed or user-defined freeze-drying process recipes, and the PC can store >10,000 programs (depending on the computer hard drive)

Eutectic point test function:

The eutectic point temperature of the product can be tested offline and online, and the spectrum can be analyzed manually or automatically (optional)

Remote control system:

The operating status of the device can be monitored remotely, and the device can be monitored in real time via WiFi or the cloud.

Mobile phone monitoring system:

You can monitor the operating status of the equipment with your mobile phone, which is simple and convenient (optional)

Cold trap defrost system:

The hot air defrosting method has high safety performance; the defrosting speed is fast, which solves the inconvenience caused by traditional immersion defrosting.

Parameter

K-MBG4

SKU: 700004319

100 assays (manual) / 400 assays (auto-analyser)

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(3¹-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Mixed linkage β-glucanase (endo-1,3:1,4-β-glucanase) / lichenase (EC 3.2.1.73) acts specifically to release 2-chloro-4-nitrophenol (CNP) from this substrate. The rate of release of CNP is directly related to the β-glucanase/lichenase activity in a sample. The reaction is terminated and the phenolate colour is developed on addition of Tris buffer solution (pH = 10.0).

Note that the substrate is not hydrolysed by β-glucosidase or cellobiohydrolase. The substrate can be hydrolysed by certain endo-cellulases (e.g. Trichoderma sp.) but this does not result in an increase in absorbance.

Discover more assay kits for enzyme activity measurement.

Data calculators are located in the Documents tab.

Advantages

• Very cost effective 
• All reagents stable for > 2 years 
• Specific for endo-1,3:1,4-β-glucanase/lichenase 
• Simple, convenient, rapid assay 
• Well suited to automation 
• Malt flour standard and lichenase standard included

The MBG4 reagent contains a single substrate, namely 4,6-O-benzylidene-2-chloro-4-nitrophenyl-β-(31-β-D-cellotriosyl-glucoside) (BCNPBG4). The benzylidene acetal group prevents any hydrolytic action by exo-acting hydrolytic enzymes such as β-glucosidase or cellobiohydrolase.

Introduction

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.

Details

Specifications

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

The sample is homogenized and lysed in the lysate, and RNA is released into the lysate. The high concentration of guanidine isothiocyanate contained in the lysate denatured and inactivated endogenous or exogenous RNase, while RNA is protected from degradation. The lysate is centrifuged to remove insoluble impurities. After adding ethanol to adjust the binding conditions, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is removed without adsorption. The column is washed with buffer VHB to remove protein and other impurities, washed with buffer RW2 to remove salt. Finally the RNA is eluted by RNase free water. The eluted RNA can be directly used in RT-PCR, Northern blot, poly-A purification, in vitro translation, etc.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Storage and Stability

Carrier RNA should be stored at 2–8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under theseconditions

Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.

Introduction

Usages:
For differentiating enteric bacteria based on urease activity by adding 40% sterile urea solution.

Principle:
Peptone provides the carbon and nitrogen; maintain a balanced osmotic sodium chloride; potassium dihydrogen phosphate is buffers; decomposing bacteria urease urea medium, produce large amounts of ammonia, agar as medium coagulant.

Formulation (per liter):
Peptone 1g
Sodium chloride 5g
Glucose 1g
Ppotassium dihydrogen phosphate 2g
Phenol red 0.012g
Agar 12g
Final pH7.2 ± 0.2

How to use:
1.Suspend 21g in 1L of distilled or deionized water. Heat with frequent agitation and boil to completely dissolve the powder. Distribute into flasks. Autoclave at 121 for 15 minutes. cooling to 50-55 and adding 40% urea solution.
2.Diluted and treated samples.

Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.

500g

Description

Our SNPsig® kits use our own proprietary genotyping method to enable the identification of SARS-CoV-2 variants of concern. These products can be used on any real-time PCR machine using familiar protocols, whilst resulting in exceptional genotyping data.

Positive control templates for wild-type and variants are supplied in every kit to make data interpretation simple.

Our SNPsig® technology provides an alternative to sequencing as well as S gene target failure (SGTF) that enables scientists to analyse and monitor these specific genomic mutations.  Our kits can provide a pivotal role in screening for SARS-CoV-2 variants for the purpose of genomic surveillance and studies.

For the detection of the SARS-CoV-2 (20H/501Y.V2 South Africa)
Rapid detection of specific detection profiles
High priming efficiency
Sensitive to < 100 copies of target Positive copy number standard curve for quantification Accurate controls to confirm findings 96 reactions, includes master mix