Description

Attogene’s Lead Rapid Lab Lateral Flow Kit can be used for the screening of Lead in water and food samples at greater than or equal to 10 ppb in a laboratory setting.  Unlike the field-based kit, the lab kit is intended for a more technical end user who will be evaluating samples in a laboratory setting.

Lead contamination is a serious worldwide environmental problem. As it is difficult to detoxify by chemical or biological methods, gradual Lead ion accumulation in the nervous and cardiovascular systems of the human body can subsequently cause serious diseases.   Long-term health consequences of drinking Lead-contaminated food and water include brain, heart, kidney, lungs  and immune system problems for adults, and the physical and mental development delays in infants and children.  Attogene’s Lead Lateral Flow test gives results conforming of 10ppb or greater in 5-15 minutes.

This is a revolutionary product designed to make Lead testing in easy and affordable.  This fast screening test kit contains 10 tests with everything needed for accurate results of unsafe lead levels. With reliable results in only 10 minutes, this test kit clearly gives results confirming Lead in water and conforming to the EPA guideline of 15 ppb (µg/L).

Sample Collection:

1. Take a first-draw sample

Immediately after opening a faucet or valve, collect a 250 mL sample. This sample should be from each tap used for consumption.

2. Take a flush sample

If first-draw sample results show elevated lead levels of 5 ppb or higher, collect a flush sample. To do this, ensure water has not been used for between 8 to 18 hours, then collect the sample at 30 seconds.

3. Take sequential samples

If you want to test a lead service line, collect 8 to 10 sequential samples, depending on how far the line is from the tap.

Standards and Regulations and recommendations for Lead

• EPA: Drinking Water: 15ppb
• FDA: Juice: 50ppb
• EPA: Residential Soil: 400 ppm (play areas), 1200 ppm (non-play areas)
• CPSC: Paint: 90ppm
• FDA: Bottled drinking water: 5ppb

High Blood lead levels (i.e., greater than 700ppb) can cause serious health effects, including seizure, coma, and death.  Blood levels as low as 100ppb have been associated with adverse effects on cognitive development, growth, and behavior among children aged 1-5 years.

Screening of Lead in water samples at 5-10 ppb
Format: 10 tests (5 tests/5 controls)
Run Time: 15 Minutes

Description 

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 10⁸–10⁹ cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 

Features

• Flexible– fresh culture, overnight culture, 4°C stored liquid culture or even colonies on agar plate can be used for transformation. 
• Fast and Easy– only few steps for preparation; suitable for time-saving transformation 
• High efficiency– up to 109 cfu/μg 
• Personalization– suitable for most E. coli strains

Kit Contents

Storage

4°C for 12 months

Champion™ E. coli Transformation Kit provides an easy method for rapid preparation of chemically competent cells with high transformation efficiency from fresh culture, overnight culture, or even directly from bacterial colonies on the plate. The competent cell preparation method eliminates the requirement of time-wasting wash step. In addition, preparation of competent cells from overnight culture or directly from bacterial colonies provides flexibility to cloning experiment. The resultant competent cells can be immediately used or stored at -70°C for one year. This kit includes a specialized SMO-Broth™ medium and a unique Champion™ CC Buffer for culturing and preparing competent cells efficiently. Following the simple and quick competent cell preparation protocol from fresh culture, the transformation efficiency is typically ranged from 108–109 cfu/μg transformants/μg of pUC19 plasmid DNA, but varies depending on the E. coli strains. The resultant competent cells can be further transformed using time-saving transformation protocol, eliminating the requirement of heat-shock and recovery steps. 

Bis-propargyl-PEG13 comprises two propargyl groups which can form triazole linkages with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The hydrophilic PEG units help improve the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Bis-propargyl-PEG13 comprises two propargyl groups which can form triazole linkages with azide-bearing compounds or biomolecules in copper catalyzed Click Chemistry reactions. The hydrophilic PEG units help improve the solubility of the molecule in aqueous media. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Specification

Overview

• Extract high quality & quantity total RNA including miRNA 
• No phenol step required; isolate all RNA in one fraction
• Genomic DNA Removal Column for efficient elimination of gDNA
• Bind & elute all RNA irrespective of size or GC content, without bias
• Efficiently extract small RNA irrespective of GC content
• Very sensitive & linear down to a few cells without the need for carrier RNA
• Convenient & fast spin column format
• Isolate from a wide variety of specimens
• Purification is based on spin column chromatography that uses Norgen’s proprietary resin separation matrix

This kit purifies total RNA including miRNA from biological samples and employs an extra column for the efficient removal of contaminating gDNA, thereby replacing the enzymantic DNase step. 

Efficiently extract total RNA from a range of samples including cells, bacteria, yeast, virus and bodily fluids including plasma/serum, blood, saliva, CSF and more.  Extract high quality and purity RNA with excellent RIN values and A260/A280 suitable for downstream applications including qRT-PCR, RT-PCR, microarrays, NGS and more.

The kit purifies all sizes of RNA from large mRNA, lncRNA down to microRNA (miRNA) in the same fraction without the requirement of phenol.  Isolate all RNA sequences at an equal rate irrespective of size. Moreover, when the RNA sequences are small (e.g. miRNA), the column binds small RNAs regardless of their GC content.

Details

Supporting Data

Figure 1 / 2

Previous

Next

Click for expanded view

Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.

• ASTM Type I Water
• Produced by reverse osmosis, ion-exchange, electro-deionization, ultrafiltration, UV treatment (UVC & 185 nm), 0.1 μm membrane filtration
• Nuclease-free, protease-free, endotoxin-free
• Suitable for molecular biology applications (qPCR, NGS etc.)
• Suitable for cell culture applications
• Produced in an ISO certified GMP laboratory
• Water system is routinely monitored for compliance with QC standards

The Norgen water purification system has been designed in conjunction with experts from Millipore Sigma. The Ultrapure NFW is purified in a two-stage process. The first stage, to produce type II water, includes pre-treatment with activated charcoal and polyphosphate, this water then undergoes reverse osmosis, electro-deionization and ion exchange to remove contaminants as well as UV treatment for disinfection. The Pure water then flows to a second system in a clean room with an additional 4 layers of purification. After passing through an additional ion exchange column, the water is then subjected to Ultrafiltration to remove particulates, bacteria, & ionic contaminants down to trace level, the water is then treated with UV light (182 nm wavelength) to break down organic matter. Lastly a second Ultrafilter for the production of pyrogen-, nuclease- and bacteria-free water at 18.2 MΩ. This water is of utmost purity and suitable for direct use in molecular biology applications such as qPCR and NGS, as well as cell culture and other applications.

Details

Applications
For use in any molecular biology application

Storage Conditions
Norgen’s Nuclease-Free Water should be stored at room temperature. Storage at +4°C or -20°C is optional.

Precautions and Disclaimers
This product is designed for research purposes only.  It is not intended for human or diagnostic use.