Introduction

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Details

Specifications

Features	Specifications
Main Functions	Extract viral RNA/DNA from 200μl plasma/serum samples
Applications	RT-PCR，PCR，NGS
Products	Viral total nucleic acid, body cell total nucleic acid, negative bacterial DNA
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Whole blood, plasma, serum, soaking solution and tissue homogenate supernatant
Sample amount	200μl
Yield	2-10μg
Elution volume	≥30μl
Time per run	≤30 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle

Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.

Advantages

• Fast – several samples can be extracted in 20 minutes by column method
• High quality – high purity total RNA can be directly used in various sensitive downstream applications
• Safe – no phenol chloroform extraction required
• Sensitive – DNA/RNA can be recovered at the level of PG
• High yield – carrier RNA contained in the product maximize the recovery of trace nucleic acid

Kit Contents

Contents	IVD4173
Purification Times	100 Preps
HiPure Viral Mini Column	100
2ml Collection Tubes	200
PK/Carrier RNA	50 mg
Protease Dissolve Buffer	5 ml
Buffer AL	30 ml
Buffer MW1	44 ml
Buffer MW2	50 ml
RNase Free Water	15 ml

Storage and Stability

This kit is shipped and stored at room temperature and is valid for 12 months.

Experiment Data

This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Overview

• Ready-to-use
• Quantitative
• Highly Stable
• Precise
• Sixteen discrete fragments ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp

The Norgen FullRanger 100 bp DNA Ladder is prepared to ensure quality and batch-to-batch consistency. Our FullRanger contains sixteen discrete fragments ranging from 100 bp to 5000 bp with higher intensity reference bands at 500 bp and 1000 bp. This Ladder is ideal for accurate visual determination in both large and small cloning applications.

Contents
1mL of premixed DNA ladder (0.5µg/10µL) in loading buffer (10mM EDTA, 10% glycerol, 0.015% bromophenol blue, and 0.17% SDS).

Details

Supporting Data

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FullRanger 100 bp DNA Ladder (Cat# 11800) – 100 loads

Ladder Properties:
• Sixteen discrete bands, ranging from 100 bp to 5000 bp
• Higher intensity reference bands at 500 bp and 1000 bp

Fragment	Size (bp)	Mass (ng)
1	5000	55
2	4000	44
3	3000	33
4	2500	28
5	2000	22
6	1500	17
7	1000	50
8	900	35
9	800	31
10	700	43
11	600	24
12	500	56
13	400	16
14	300	22
15	200	15
16	100	11

Recommended Use:

Mix thoroughly. For best results, load 10µL of DNA ladder per well. For precise mass determination with a densitometer, stain gel after electrophoresis using 0.5µg/mL ethidium bromide for 30-40 minutes. The table above shows the size and mass for each band based on 10µL ladder per well.

Storage: 

Stable at room temperature. For longer term storage, -20°C is recommended.

This ladder was standardized using 10µL of DNA per lane on a 0.8 cm thick, 13 x 15 cm, 1.0% agarose gel run in TAE buffer.

Overview

• Detection kits for ZIKV
• CE-IVD marked version available for in vitro diagnostic use
• Available in TaqMan format for analysis

The Zika Virus (ZIKV) is an emerging mosquito-borne virus that was first identified in Uganda in 1947 in Rhesus monkeys through monitoring of sylvatic yellow fever. During large outbreaks in French Polynesia and Brazil, national health authorities reported potential neurological and auto-immune complications of ZIKV disease. Agencies investigating the Zika outbreaks are finding an increasing body of evidence about the link between ZIKV and microcephaly. Infection with ZIKV may be suspected based on symptoms and recent history (e.g. residence or travel to an area where ZIKV is known to be present). Zika virus diagnosis can only be confirmed by laboratory testing for the presence of ZIKV RNA in the blood or other body fluids, such as urine or saliva.

ZIKV TaqMan RT-PCR Kit, 100 reactions

• Ready to use format, including Master Mix for the target and PCR control to monitor for PCR inhibition and validate the quality
• Specific Primer and Probe mix for the pathogen/virus/viroid of interest
• Primer and Probe mix
• Positive and negative control to confirm the integrity of the kit reagents

ZIKV TaqMan RT-PCR Probe/Primer Set and Controls, 100 reactions

• Specific Primer/Probe mix and Positive Control for the pathogen/virus/viroid of interest
• Nuclease-free water
• Can be used together with Norgen’s RT-PCR Master Mix (#28113) or customer supplied master mix

Details

Supporting Data

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Storage Conditions

All kit components can be stored for 1 year after the date of production without showing any reduction in performance.

All kit components should be stored at -20°C upon arrival.

Component	Cat. TM62250 (100 preps)	Cat. TM62210 (100 preps)
MDx TaqMan 2X PCR Master Mix	2 x 700 μL	–
ZIKV Primer & Probe Mix	280 μL	280 μL
ZIKV Positive Control	150 μL	150 μL
Nuclease-Free Water (Negative Control)	1.25 mL	1.25 mL
Product Insert	1	1