Norgen’s PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) provides a rapid, simple and efficient procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on magnetic beads without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
Norgen’s PCR and Sequencing Reaction Clean-Up Kit (Magnetic Bead System) is also available in a 96-well format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
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Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 60200 (50 preps) | Cat. 62700 (192 preps) |
|---|---|---|
| Binding Buffer C | 30 mL | 2 x 30 mL |
| Elution Buffer B | 8 mL | 15 mL |
| Magnetic Bead Suspension | 1.1 mL | 8.5 mL |
| Elution Tubes (1.7 mL) | 50 | – |
| 96-Well Elution Plate | – | 2 |
| Adhesive Tape | – | 2 |
| Product Insert | 1 | 1 |
K-MANOL
SKU: 700004317
60 assays (manual) / 600 assays (microplate) / 580 assays (auto-analyser)
| Content: | 60 assays (manual) / 600 assays (microplate) / 580 assays (auto-analyser) |
| Shipping Temperature: | Ambient |
| Storage Temperature: | Short term stability: 2-8oC, Long term stability: See individual component labels |
| Stability: | > 2 years under recommended storage conditions |
| Analyte: | D-Mannitol |
| Assay Format: | Spectrophotometer, Microplate, Auto-analyser |
| Detection Method: | Absorbance |
| Wavelength (nm): | 340 |
| Signal Response: | Increase |
| Linear Range: | 5 to 75 μg of D-mannitol per assay |
| Limit of Detection: | 0.50 mg/L |
| Reaction Time (min): | ~ 4 min |
| Application examples: | Wine, chewing gum, dietetic foods, candies, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.). |
| Method recognition: | Novel method |
The D-Mannitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol in food, beverages and other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
View our complete list of alcohol assay kits.
Advantages
The D-Mannitol test kit is suitable for the rapid and specific measurement and analysis of D-mannitol in food, beverages and other materials.
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.
Specifications
| Features | Specifications |
| Main Functions | Isolation total DNA from100 mg simple plant |
| Applications | PCR, SSR, AFLP, RAPD and southern blot, etc. |
| Purification method | Mini spin column |
| Purification technology | Silica technology |
| Process method | Manual (centrifugation or vacuum) |
| Sample type | Economic plant samples |
| Sample amount | Fresh / frozen plant samples: ≤100mggDried plant / seed samples: ≤20mg |
| Elution volume | ≥30μl |
| Time per run | 30-50 minutes |
| Liquid carrying volume per column | 800μl |
| Binding yield of column | 100μg |
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane. The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.
Kit Contents
| Contents | D316402 | D316403 |
| Purification Times | 50 Preps | 250 Preps |
| RNase A | 10 mg | 50 mg |
| Protease Dissolve Buffer | 1.8 ml | 5 ml |
| Buffer SPL | 30 ml | 150 ml |
| Buffer PS | 10 ml | 50 ml |
| Buffer GW1 | 22 ml | 110 ml |
| Buffer GW2* | 12 ml | 2 x 50 ml |
| Buffer AE | 15 ml | 60 ml |
| HiPure gDNA Mini Columns | 50 | 2 x 125 |
| 2 ml Collection Tubes | 50 | 2 x 125 |
Storage and Stability
RNase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
Experiment Data
| Name | CAT NO | Fresh / frozen tissue amount | Dried tissue amount | Column type | Elution volume | Yield (muscle) |
| HiPure Plant DNA Mini Kit | D3187 | 150mg | 40mg | 1.5ml column | 50 – 100μl | 3-70μg |
| HiPure HP Plant DNA Maxi Kit | D3163 | 5g | 1g | 50ml column | 0.7 – 3ml | 75-1250μg |
| HiPure SF Plant DNA Kit | D3164 | 100mg | 20mg | 1.5ml column | 50 – 100μl | 3-50μg |
| HiPure SF Plant DNA 96 Kit | D3167 | 50mg | 15mg | 96 well plate | 75 – 150μl | 3-30μg |
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.