Introduction

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 1-1.5ml whole blood, 0.5-1ml bone marrow, buffy coat
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount	1-1.5ml whole blood, 0.5-1ml bone marrow
Yield	1-30μg

Principle

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples. This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR,NGS and virus detection.

Advantages

• High purity – OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy – less than 50% of the price of top brands
• Automatic – extraction without manual participation, saving time and effort
• High yield – nucleic acid recovery up to 95%
• General – samples include whole blood, serum, plasma, lymphocyte suspension, bone marrow, cultured cells, etc.

Kit Contents

Contents	R661101	R661102	R661103
Purification Times	48 Preps	96 Preps	480 preps
10 x RBC Lysis Buffer	50 ml	2 x 50 ml	4 x 100 ml
Proteinase K	12 mg	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	1.8 ml	10 ml
DNase I	600 μl	2 x 600 μl	10 x 600 µl
DNase Buffer	20 ml	30 ml	150 ml
MagPure Particles N	1.2 ml	2.5 ml	11 ml
Buffer RTL	30 ml	60 ml	300 ml
Buffer ALB2	40 ml	60 ml	300 ml
Buffer MW1*	22 ml	44 ml	220 ml
Buffer MW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	20 ml	60 ml

Storage and Stability

DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N and Proteinase K should be stored at 2–8°C upon arrival. However, short-term storage(up to 8 weeks) at room temperature (15–25°C) does not affect their performance.The remaining kit components can be store at room temperature and are stable for up to 18 months under these conditions.

This product is suitable for extracting RNA from anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells and other samples.

Introduction

Usages:
Yeast extract is made from yeast, used in microbiological culture media as a source of nitrogen and B vitamins.

Technical specification:
Total nitrogen(TN)—————————≥10.0%
Amino nitrogen(AN) ————————≥4.0%
Moisture—————————————≤5.0%
Ash———————————————≤10.0%
Clˉ——————————————–＜2.0%


Storage: Keep container tightly closed, store in a cool, dry place, away from bright light.

Specifications: 500g/bottle; 10kg/bag

500g/bottle; 10kg/bag

 K-LMALAF

SKU: 700004312

245.5 mL of prepared reagent (e.g. 1116 assays of 0.22 mL)

Content:	245.5 mL of prepared reagent (e.g. 1116 assays of 0.22 mL)
Shipping Temperature:	Ambient
Storage Temperature:	Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:	> 2 years under recommended storage conditions

Analyte:	L-Malic Acid
Assay Format:	Auto-analyser
Detection Method:	Absorbance
Wavelength (nm):	340
Signal Response:	Increase
Linear Range:	up to 80 μg/mL of L-malic acid in final reaction solution
Limit of Detection:	20 mg/L
Reaction Time (min):	~ 3 min
Application examples:	Wine, beer, fruit juices, soft drinks, candies, fruit and vegetables, bread, cosmetics, pharmaceuticals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:	Methods based on this principle have been accepted by AOAC, EEC, EN, NF, NEN, DIN, GOST, OIV, IFU, AIJN, NBN, ISO and MEBAK

The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.

View more assay kits on our organic acid list.

Advantages

• Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4^oC.
• PVP incorporated to prevent tannin inhibition 
• Very stable reagent when prepared for auto-analyser applications 
• Linear calibration (R² ~ 0.9994) up to 80 μg/mL of L-malic acid in final reaction solution 
• Validated by the University of Wine, Suze la Rousse, France 
• Very competitive price (cost per mL of reagent) 
• Both enzymes supplied as stable suspensions 
• Very rapid reaction (~ 3 min)

The L-Malic Acid (Analyser Format) test kit is an analyser format for the specific measurement and analysis of L-malic acid (L-malate) in beverages and food products. On calibration, the prepared reagent is linear to > 80 micrograms of L-malic acid per mL of assay solution.