The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Detail
K-ASNAM
SKU: 700004266
100 assays (50 of each) per kit
Content:
100 assays (50 of each) per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 6 months under recommended storage conditions
Analyte:
Ammonia, L-Asparagine, L-Glutamine
Assay Format:
Spectrophotometer, Microplate
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
0.2 to 7.0 µg of ammonia, or 0.5 to 50 μg of L-asparagine or L-glutamine per assay
Potatoes, potato products, vegetables, cereals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Novel method
The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Plasmid DNA is ready for various downstream applications including restriction digestion, bacterial transformation, sequencing and more
Available in 4 formats: MiniPrep, MiniPrep (Magnetic Bead System), 96-Well MiniPrep (Magnetic Bead System), and MaxiPrep
These kits are designed for the rapid preparation of plasmid DNA from Escherichia coli.
Plasmid MiniPrep Kit
This kit is designed for the rapid preparation of plasmid DNA from small cultures of Escherichia coli using convenient spin columns. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. This kit is able to purify plasmids up to 13,000 bp in size, and the typical purification yield is up to 20 μg from 1.5 mL of bacterial culture. Purified DNA is of excellent quality for transformation, restriction enzyme digestion, sequencing and more. Also available in a 96-well format.
Plasmid MiniPrep Kit (Magnetic Bead System and High Throughput Magnetic Bead System)
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is designed for the rapid preparation of plasmid DNA from small batch cultures of Escherichia coli. Norgen’s magnetic beads bind DNA under optimized salt concentrations and release the bound DNA under low salt and slightly alkali conditions. The plasmid DNA is preferentially purified from other cellular components such as genomic DNA and RNA. The purified plasmids are fully digestible with all restriction enzymes tested, and are completely compatible with real-time PCR and NGS.
Norgen’s Plasmid MiniPrep Kit (Magnetic Bead System) is also available in a 96-well (HT) format for high throughput applications. Purification with the 96-well plates can be integrated with a robotic automation system.
Plasmid MaxiPrep Kit
This kit is designed for the rapid spin column preparation of plasmid DNA from up to 100 mL of Escherichia coli cultures. The kit allows for the isolation of plasmid DNA with final endotoxin levels of 0.1 EU/µg of DNA or less. The kit is able to purify plasmids up to 13,000 bp in size, and typical yields from a 100 mL culture for a high copy number plasmid are between 0.4 and 1.0 mg. The purified DNA is fully digestible with all restriction enzymes tested, and is completely compatible with manual or automated sequencing to achieve 95-100% accuracy.
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the of shipment. The RNase should be stored at -20°C upon arrival. The Resuspension Solution AZ should be stored at 4°C upon addition of RNase enzyme.
Component
Cat. 13300 (50 preps)
Cat. 46400 (250 preps)
Cat. 46500 (4 preps)
Cat. 46600 (20 preps)
Cat. 60300 (50 preps)
Cat. 63000 (192 preps)
Resuspension Solution AZ
12 mL
60 mL
20 mL
100 mL
12 mL
2 x 20 mL
Lysis Buffer N
40 mL
80 mL
40 mL
2 x 80 mL
40 mL
2 x 40 mL
Buffer TN
20 mL
130 mL
55 mL
2 x 130 mL
20 mL
1 x 55 mL 1 x 20 mL
Wash Solution E
12 mL
2 x 18 mL
–
–
–
–
Elution Buffer K
8 mL
30 mL
–
–
8 mL
2 x 8 mL
Wash Solution J
–
–
25 mL
3 x 25 mL
–
–
Elution Buffer J
–
–
24 mL
120 mL
–
–
RNase A
1 vial
1 vial
1 vial
1 vial
1 vial
1 vial
Magnetic Bead Suspension
–
–
–
–
1 x 1.1 mL
4 x 1.1 mL
Spin Columns
50
250
–
–
–
–
Collection Tubes
50
250
–
–
–
–
DNA Maxi Spin Columns with Collection Tubes (Clear ring in column)
–
–
4
20
–
–
Maxi Spin Filter Columns with Collection Tubes (Grey ring in column)
Usages： For determination of coliform confirmatory test for multi-tube fermentation.
Principle: Peptone provide carbon and nitrogen sources; lactose as fermentable sugars; ox bile powder and brilliant green inhibit the non-Enterobacteriaceae.
Formulation (per liter): Peptone: 10g Lactose: 10g Ox bile powder: 20g Brilliant green: 0.0133g Final pH7.2 ± 0.2
How to use: 1.Suspend 40g of the product, add 1 L of distilled water, stirring heated to boiling until completely dissolved, packed in a test tube with a small inverted tube, autoclave at 121 ℃ for 15min, cool to room temperature and set aside. 2.Sample handling and dilution. Slightly. 3.Transplanted LST broth tube to brilliant green lactose bile broth tube by using a inoculating loop. 4.the tube was placed in an incubator culture at 36 ± 1 ℃ for 24h. 5.Observe the results. Press production trachea number, check table report MPN MPN value per gram (mL) sample of coliform bacteria.
Quality control: Quality control strains were inoculated and culuture at 36 ± 1 ℃ for 24h ,results are as follows: Bacterial Name Bacterial No. Growth Status Gassing Escherichia coli ATCC25922 good +
Salmonella typhimurium CMCC (B) 50115 good —
Staphylococcus aureus ATCC6538 inhibited —
Storage: Store in a dark, cool and dry place, tighten the cap immediately after use. Storage period of three years.
