The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Detail
K-ASNAM
SKU: 700004266
100 assays (50 of each) per kit
Content:
100 assays (50 of each) per kit
Shipping Temperature:
Ambient
Storage Temperature:
Short term stability: 2-8oC, Long term stability: See individual component labels
Stability:
> 6 months under recommended storage conditions
Analyte:
Ammonia, L-Asparagine, L-Glutamine
Assay Format:
Spectrophotometer, Microplate
Detection Method:
Absorbance
Wavelength (nm):
340
Signal Response:
Decrease
Linear Range:
0.2 to 7.0 µg of ammonia, or 0.5 to 50 μg of L-asparagine or L-glutamine per assay
Potatoes, potato products, vegetables, cereals and other materials (e.g. biological cultures, samples, etc.).
Method recognition:
Novel method
The L-Asparagine/L-Glutamine/Ammonia test kit is a novel method for the specific, convenient, cost effective and rapid measurement and analysis of L-asparagine, L-glutamine and ammonia as acrylamide precursors in the food industry, or as cell culture media/supernatant components, or in other materials.
Note for Content: The number of manual tests per kit can be doubled if all volumes are halved. This can be readily accommodated using the MegaQuantTM Wave Spectrophotometer (D-MQWAVE).
Extended cofactors stability. Dissolved cofactors stable for > 1 year at 4oC.
Very rapid reaction due to use of uninhibited glutamate dehydrogenase
All enzymes supplied as stabilised suspensions
Only kit available
Very cost effective
All reagents stable for > 2 years after preparation
Mega-Calc™ software tool is available from our website for hassle-free raw data processing
Standard included
Other Products
R4182 HiPure Microbial RNA Kit
Product Info
Document
Product Info
Introduction
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Details
Specifications
Features
Specifications
Main Functions
Isolation total RNA from bacteria, yeast cells
Applications
RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation
Purification method
Mini spin column
Purification technology
Silica technology
Process method
Manual (centrifugation or vacuum)
Sample type
Bacteria, yeast cells
Sample amount
Bacteria: <10^9；Yeast cells：<2 x10^7
Elution volume
≥30μl
Time per run
≤40 minutes
Liquid carrying volume per column
800µl
Binding yield of column
100µg
Principle
Hipure silica gel column is based on glass fiber filter membrane with high binding force. Under the condition of high concentration of ionizing agent (such as guanidine hydrochloride or guanidine isothiocyanate), the filter membrane can adsorb nucleic acid through hydrogen bond and electrostatic, while protein and other impurities are not adsorbed and removed. The filter membrane adsorbed with nucleic acid is washed to remove the residual protein and salt. Finally, the nucleic acid adsorbed on the filter membrane can be washed out with low salt buffer (such as buffer TE) or water. The obtained nucleic acid has high purity and can be directly used in various downstream experiments.
Advantages
Fast – several samples can be extracted in 30 minutes
High purity – the purified RNA can be directly used in various downstream applications
High recovery – RNA can be recovered at the level of PG
Good repeatability – silica gel column purification technology can obtain ideal results every time
Broad spectrum – it can deal with various bacteria, including Gram-positive bacteria that are difficult to be lysed
Sufficient components – the kit contains carried wall breaking enzymes and glass beads
Kit Contents
Contents
R418202
R418203
Purification Times
50 Preps
250 Preps
HiPure RNA Mini Columns
50
250
2ml Collection Tubes
50
250
Glass Beads (0.1-0.6mm)
30 g
150 g
DNase I
600 μl
5 x 600 μl
DNase Buffer
6 ml
30 ml
Protease Dissolve Buffer
1.8 ml
15 ml
Buffer ATL
50 ml
200 ml
Buffer PHC
50 ml
200 ml
Buffer GXP2*
20 ml
100 ml
Buffer RW1
50 ml
250 ml
Buffer RW2*
20 ml
2 x 50 ml
RNase Free Water
10 ml
30 ml
Storage and Stability
Buffer PHC should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage (DNase I up to 1 weeks, Buffer PHC up to 12 weeks) at room temperature(15–25°C) does not affect their performance. The remaining kit components can be stored at roomtemperature (15–25°C) and are stable for at least 18 months under these conditions.
Document
Hipure Microbial RNA kit is suitable for extracting high-purity total RNA from bacterial/yeast culture medium. This kit combines high-efficiency Magzol Reagent (one-step extraction reagent) and silicagel column purification technology to complete the extraction of high-purity total RNA in only 40 minutes. The obtained RNA can be directly used in RT-PCR, Northern blot, poly-A + purification, nucleic acid protection and in vitro translation.
Compact and stylish designed, the BioShake iQ high-end thermoshaker lets you perform all your standard mixing runs with a minimum of adjustments. The BioShake iQ offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
For microplates, tubes and glass vials
Fast & high-precision heating
Temperature range: ambient to 99°C
Precise mixing from 200 – 3,000 rpm
Wide range of thermo adapters
Stylish aluminium housing
ADVANCED TECHNOLOGY
Save time.
By combining the mixing operation with the incubation phase, reaction process times and operator workload are reduced and efficiency of many procedures is increased, resulting in a higher throughput.
The first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake iQ thermoshaker offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
HOMOGENEOUS HEATING
Using large-area heating source in combination with digital sensor electronic
The BioShake iQ heats from ambient to 99°C in a short time, provides ease of use features, amazing comfort and maximum safety.
Within the BioShake iQ thinnest large-area heating elements and sensors are integrated. During the heat up time the special electronic control system guarantees the same temperature for all vessels with minimal deviations. Temperatures are selectable from ambient temperature to 99°C in steps of 1.0°C. The nominal regulation accuracy is better than ±0.1°C with a uniformity of temperature distribution better than ±0.5°C at 45°C across the heating surface.
Exchangeable thermo adapter plates and thermal blocks enable a perfect adaptation to microplates, tubes or glass vials.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
COMFORT
Simple intuitive operation and programming
The BioShake iQ is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time, mixing frequency and temperature.
EXCHANGABLE THERMO ADAPTERS
For a wide range of applications
The BioShake iQ comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels. An excellent temperature uniformity and homogeneity is guaranteed for all samples.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake iQ its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
Document
For microplates, tubes and glass vials Fast & high-precision heating Temperature range: ambient to 99°C Precise mixing from 200 – 3,000 rpm Wide range of thermo adapters Stylish aluminium housing
Available in 3 formats: Mini, Midi, and Maxi to suit your desired input volume
Remove endotoxins from up to 1 mg of DNA with the Maxi format kit
Fast and easy processing using a rapid spin-column format
These kits are designed for the rapid spin column removal of endotoxins from previously purified DNA. Norgen’s spin columns bind DNA while endotoxins, salts and other contaminants are washed away. These kits reduce endotoxins to 0.1 EU/μg DNA or less providing plasmid DNA that is immediately ready for transfections or other endotoxin-sensitive applications. Typical recovery of DNA is >90% of the starting sample.
Endotoxin Removal Kit (Mini)
This kit is designed for the rapid spin column removal of endotoxins from up to 25 µg of previously purified DNA. The convenient spin column procedure can be completed in approximately 20 minutes.
Endotoxin Removal Kit (Midi)
This kit is designed for the rapid spin column removal of endotoxins from up to 200 μg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
Endotoxin Removal Kit (Maxi)
This kit is designed for the rapid spin column removal of endotoxins from up to 1 mg of previously purified DNA. The convenient spin column procedure can be completed in approximately 30 minutes.
About Endotoxins
Endotoxins (also called lipopolysaccharides), are cell-membrane components of Gram-negative bacteria such as E. coli. Endotoxins are released during the lysis step of plasmid purification and significantly reduce transfection efficiencies in endotoxin sensitive cell lines. Therefore, the removal of endotoxins from plasmid preparations is often necessary prior to the use of the DNA in such downstream applications.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 2 years after the date of shipment.
