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Low Abundance RNA Quantification Kit

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Product Info

Overview

Quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range
Compatible with any Real-Time PCR system
RNA is accurately quantified using a standard curve constructed from the provided RNA standard

Norgen’s Low Abundance RNA Quantification Kit offers a PCR-based detection procedure to quantify RNA of a wide spectrum of concentrations, including the lower ng per µL and pg per µL range. The kit has two main enzymatic components – reverse transcription using Norgen’s microScript Reverse Transcription system and PCR Master Mix used in conjunction with a specially formulized primer mixture, to amplify human RNA from different types of inputs (such as various liquid biopsies). The kit is compatible with any Real-Time PCR system with the addition of fluorescent nucleic acid stains such as SYBR Green. The unknown RNA is accurately quantified by using a standard curve constructed from the provided RNA Standard.

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Supporting Data

Figure 1 / 4

Figure 1. Sensitivity of Norgen's Kit Compared to Other Methods

Figure 2. Sensitive RNA quantification wide dynamic range of concentrations

Figure 3. Sensitive RNA Quantification from Small Volumes in the Picogram Range

Figure 4. Sensitive RNA Quantification from Small Volumes of Plasma

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Storage Conditions
Upon receipt, store Norgen’s Low Abundance RNA Quantification Kit at -20°C or lower. Avoid multiple freeze-thaw cycles. If needed, prepare smaller working aliquots and store at -20°C or lower.

Component	Cat. 58900 (48 reactions)
microScript microRNA Enzyme Mix	20 μL
2x microScript Reverse Transcriptase Reaction Mix	0.5 mL
2X Real-Time PCR Master Mix	1 mL
RNA Quantification Primer Set Mix	200 μL
Quantified RNA Standard 50 pg/μL	2 x 60 μL
Nuclease-Free Water	3 x 1.25 mL
Product Insert	1