Introduction

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Details

Specifications

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA – meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
• Fast – without separation of leukocytes, organic extraction or ethanol precipitation
• Simple – all nucleic acids can be obtained by direct digestion
• Wide applicability- handle a variety of liquid samples

Kit Contents

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

Experiment Data

This product is suitable for rapid extraction of total DNA from tissue, cells, blood, saliva, swabs, blood spots, semen and other clinical samples. DNA can be used directly for PCR, quantitative PCR, Southern Blot, test of virus DNA and so on.

Description 

The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease. 

Composition 

• 0.03% Xylene cyanol FF
• 0.03% Bromophenol blue 
• 10 mM Tris-HCl (pH 8.0) 
• 60% glycerol 
• 60 mM EDTA

Storage 

4°C for 12 months
-20°C for 36 months

The ExcelDye™ 6× DNA Loading Dye (Blue) is pre-mixed buffer for tracking the DNA sample during the electrophoresis on agarose or polyacrylamide gels. It contains two dyes (Xylene cyanol FF and Bromophenol blue) for tracking the DNA migration. The Xylene cyanol FF and Bromophenol blue migrate at approximately 800 bp and 150 bp on a standard 2% TAE agarose gel respectively (4,000 bp and 500 bp on 1% TAE agarose gel respectively). The included glycerol keeps the DNA at the bottom of the well and the presence of EDTA chelates divalent metal ions to prevent the process of metal-dependent nuclease. 

Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG17-methane has an alkyne group which can participate in Click Chemistry reactions with azide compounds to yield a stable triazole linkage; copper is required as a catalyst. The hydrophilic PEG units increases solubility of the molecules in aqueous environment. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.