Hydroxy-PEG3-DBCO is a PEG linker containing a DBCO moiety and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Hydroxy-PEG3-DBCO is a PEG linker containing a DBCO moiety and a terminal primary hydroxyl group. The hydroxyl can react with a variety of functional groups and the hydrophilic PEG spacer arm can provide better solubility to labeled molecules. DBCO is commonly used for copper-free Click Chemistry reactions. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
This kit provides a fast, reliable and simple procedure for high throughput isolation of DNA from all types of soil samples including common soil samples and difficult soil samples with high humic acid content such as compost and manure. A combination of chemical and physical homogenization effectively lyses all microorganisms in the soil sample, and the kit removes all traces of humic acid using the provided Organic Substance Removal (OSR) Solution and Humic Acid Removal plate (HAR). Total genomic DNA can be isolated and purified from all the various microorganisms found in soil, such as bacteria, fungi and algae. The purified DNA is of the highest quality and is fully compatible with downstream PCR and qPCR applications and more for any metagenomic study, as all humic acid substances and PCR inhibitors are removed during the isolation.
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| Kit Specifications | |
| Binding Capacity Per Well | 50 μg |
| Maximum Loading Volume Per Well | 500 μL |
| Size of DNA Purified | All sizes |
| Maximum Amount of Starting Material | 250 mg |
| Time to Complete 96 Purifications | 50 minutes |
Storage Conditions and Product Stability
All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
| Component | Cat. 26560 (192 preps) |
|---|---|
| Lysis Buffer G | 2 x 100 mL |
| Lysis Additive A | 25 mL |
| Binding Buffer I | 25 mL |
| OSR Solution | 12 mL |
| Wash Solution A | 2 x 38 mL |
| Elution Buffer B | 30 mL |
| Bead Tubes | 200 |
| HAR Plate | 2 |
| 96-Well Filter Plate | 2 |
| Adhesive Tape | 4 |
| 96-Well Collection Plate | 4 |
| 96-Well Elution Plate | 2 |
| Product Insert | 1 |
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection of numerous significant pathogens. The genesigPLEX range provides the most efficient method of detecting DNA and RNA by combining multiple assays into a single tube. This principle of multiplexing represents state of the art PCR testing and is a key feature of the high-throughput testing conducted by laboratories around the world. These assays include process controls to verify the quality of the nucleic acid extraction and eliminate false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the handbook.
Packaged, optimised and ready to use. Expect Better Data.
Precise reproducible results
Time saving through completing multiple tests in one go
Cost saving through a reduced price compared with buying four kits separately
Exceptional value for money
Supplied lyophilised – no cold chain shipping
Nucleic acid testing (NAT) is the method of choice for detection and quantification of a wide range of micro organisms. Primerdesign manufactures and supplies high quality quantitative real-time PCR kits for the detection and simultaneous quantification of numerous significant pathogens . A copy number standard curve is provided for quantification and an the internal extraction template (DNA or RNA), controls for the quality of the nucleic acid extraction and eliminates false negative results.
The kit is designed with the broadest possible detection profile to ensure that all clinically relevant strains and subtypes are detected. Target sequences are selected by working with data from key opinion leaders in the field. Multiple sequence alignments and unprecedented real-time PCR expertise in design and validation ensure the best possible kit.
Details of the target and priming specificity are included in the individual handbooks above.
Packaged, optimised and ready to use. Expect Better Data.
Exceptional value for money
Rapid detection of all clinically relevant subtypes
Positive copy number standard curve for quantification
Highly specific detection profile
High priming efficiency
Broad dynamic detection range (>6 logs)
Sensitive to < 100 copies of target
Accurate controls to confirm findings
50 & 150 reactions
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Tel : 081-875-1869 , 02-328-7179
Email : hej@a3p-scientific.com
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