Methyltetrazine-DBCO, TEA salt

Introduction

Usages:

For confirmatory test of colifrom in water.

Principle:

Peptone, yeast extract powder, beef extract powder provides carbon and nitrogen sources, vitamins and minerals; lactose as fermentable sugars; agar as medium coagulant; potassium hydrogen phosphate as buffer.

Formulation(per liter):

Peptone 10g

Yeast extract powder 5g

Beef extract powder 5g

Lactose 10g

Agar 15g

Dipotassium hydrogen phosphate 3.5g

Sodium sulphite 5g

Fuchsin 1g

Final pH 7.2 ± 0.2

How to use:

1.Suspend 54.5g in 1 L of distilled water , stirring heated to boiling until completely dissolved, dispensing flask, autoclave at 115 for 20min. Cool to 50 and pour into petri dishes.

2.Diluted and treated samples.

Storage: Keep container tightly closed, store in a cool, dry place, away from bright light. Storage period of 3 years.

500g

Introduction

This product is suitable for extracting RNA  from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents. It can specially extract high quality RNA from frozen blood samples.

Details

Specifications

Features	Specifications
Main Functions	Isolation total RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents.
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	anticoagulant blood, lymphocytes, buffy coat, bone marrow, cultured cells
Sample amount	1ml whole blood (fresh or frozen blood)

Principles

This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested by lysis buffer and protease, and RNA/DNA is released into the lysis buffer. Add binding solution and magnetic particles to adsorb RNA/DNA, while proteins are not adsorbed and removed. The particles adsorbed with DNA/RNA are washed with washing buffer to remove proteins and other impurities, then washed with ethanol to remove salt, and finally digested with DNase to remove DNA. RNA is recovered by adding binding solution, and finally the RNA is eluted with low salt buffer. The eluted RNA can be directly used for experiments such as RT-PCR, NGS and virus detection.

Kit Contents: Bottle

Contents	R661301	R661302	R661303
Purification Times	48 Preps	96 Preps	480 preps
DNase I	600 μl	2 x 600 μl	10 x 600 µl
DNase Buffer	20 ml	30 ml	150 ml
MagPure Particles N	2.5 ml	5.0 ml	28 ml
MagZol 3BD	65 ml	140 ml	3 x 200 ml
Buffer ALB2	40 ml	60 ml	350 ml
Buffer BCP2	10 ml	15 ml	80 ml
Buffer MW2*	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	20 ml	60 ml

Storage and Stability

DNase I should be shipped with ice pack or dry ice and stored at -20°C upon arrival. MagPure Particles N, MagZol 3BD and Buffer BCP2 should be stored at 2–8°C upon arrival. DNase I should be stored at -20°C. However, short-term storage at room temperature (15–25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15–25°C) and are stable for at least 18 months under these conditions.

This product is suitable for extracting RNA from 1ml anticoagulant blood (fresh or frozen blood/cryopreservation blood), lymphocytes, buffy coat, bone marrow, cultured cells, etc using Magzol reagents. It can specially extract high quality RNA from frozen blood samples.

Propargyl-PEG9-amine has a propargyl group and an amine group. The amine group reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) to form amide bonds. The propargyl group reacts with azides to form triazole linkage with the presence of copper as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.

Propargyl-PEG9-amine has a propargyl group and an amine group. The amine group reacts with carboxylic acids, activated NHS esters, carbonyls (ketone, aldehyde) to form amide bonds. The propargyl group reacts with azides to form triazole linkage with the presence of copper as a catalyst. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.