Purify sequencing extension products from dye terminators, primers and other contaminants
Also purify DNA from different enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations.
High recovery
Fast and efficient spin column format
Also available in 96 well format for high throughput
This kit provides a rapid spin column procedure for the purification and clean-up of sequencing and various other enzymatic reactions including restriction enzyme digests, Klenow reactions, alkaline phosphatase reactions, and ligations. The kit is used to remove reaction contaminants including dye terminators, salts, enzymes, excess primers and primer dimers. Contaminants are undesirable as they can interfere with many downstream applications including sequencing, RFLP, restriction enzyme digestions and ligation. Purification is based on spin-column chromatography without the use of phenol, chloroform or alcohol precipitation. The kit provides a high quality product with up to 90% recovery.
The kit is also available in a 96-well format for high-throughput sequencing reaction clean-up. Purification with the 96-well plate can be performed using either a vacuum manifold or centrifugation.
Storage Conditions and Product Stability All solutions should be kept tightly sealed and stored at room temperature. This kit is stable for 1 year after the date of shipment.
Component
Cat. 34500 (50 preps)
Cat. 34400 (2 x 96-well plate)
Binding Buffer C
30 mL
3 x 30 mL
Wash Solution A
12 mL
2 x 20 mL
Elution Buffer B
8 mL
2 x 15 mL
Spin Columns
50
–
Collection Tubes
50
–
Elution Tubes (1.7 mL)
50
–
96-Well Plate
–
2
Adhesive Tape
–
4
96-Well Collection Plate
–
2
96-Well Elution Plate
–
2
Product Insert
1
1
Other Products
High-speed lab mixer for microplates & tubes
Product Info
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Product Info
Compact and stylish designed, the BioShake XP high-speed microplate mixer lets you perform all your standard mixing runs with a minimum of adjustments. The XP offers outstanding performance to handle a wide range of applications across biotechnology, pharmaceutical and academic research.
Small. Light. Efficient. Quiet.
Ideal for microplates & PCR plates
High-precision mixing from 200 up to 3,000 rpm
Universal spring clamps for easy fixation
Wide range of adapters
Stylish, fire resistant aluminium housing
ADVANCED TECHNOLOGY
Compact & stylish designed
Mixing tasks in microplates can be realized fast & safe with using adjustable speed of 200 up to 3,000 rpm.
The BioShake mixing-technology is obviously more robust, vibration free and needs less maintenance compared to classical mixers.
The first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
GENTLE MIXING OF SAMPLES
By planar orbital motion
German designed and manufactured, BioShake XP plate mixer offer an ultra-efficient, 2-dimensional shaking axis so that samples mix completely in a fraction of the time of competing systems.
The mixing orbit of 2.0 mm is always constant. The orbital shaking is precisely controlled, in fact, that you need never spin down your plates after mixing. Even tubes, vials, high density plates, or low sample volumes, offer no obstacle for these precision tools.
Fully adjustable between 200 rpm and 3,000 rpm, well beyond the speeds of most other brands, guarantees fast, splatter-free, mixing for tubes, glass vials or across an entire 384-well microplate.
COMFORT
Simple intuitive operation and programming
The BioShake XP is operated from the front face of the enclosure which includes touch buttons and a display.
Special buttons for start, stop and storage of time and mixing modes enable the instrument to run complex applications. This opens new points of view on the daily laboratory work and optimizes routine application enormously.
The short mix button allows short and fast mixing in between.
The two line LCD display guarantees simultaneous and safe reading of all programmed and measured parameters as time and mixing frequency.
UNIVERSAL SPRING CLAMPS
Suitable for all microplates
In the corners eight spring clamps are inserted for easy fixation of skirted microplates anddeep well plates in SBS format 127.7 x 85.5 mm without an attached adapter. These spring clamps lock the microplate quickly and safely, even at the highest mixing frequencies.
For flexible and deformable PCR plates we recommend using of the related PCR adapter.
Fast mixing processes with frequent plate changes can be performed in the routine as easy and safe.
UNIQUE DESIGN MEETS HIGH-END TECHNOLOGY
First-class finished aluminium housing
Unique and efficient design combined with the most compact housing result in beautiful and well-defined laboratory equipment. Therefore, the first-class finished aluminium housing gives the BioShake XP its essential functionality. It provides a high amount of security, device stability and ensures a long service life.
Safety of the user and ease of use are clearly a priority for us.
EXCHANGABLE ADAPTERS
For a wide range of applications
The BioShake XP comes with a variety of standardized and specific adapter plates. The replacement of the adapters are very simple.
Perfect shaped adapters allow an optimal fit for standard tubes, lysis tubes, microplates, glass vials and other sample vessels.
MADE IN GERMANY
High-end instruments for daily routine
A perfectly harmonious blend of high-tech and handmade. “Made in Germany” has always been a recipe for success for QInstruments. 100% of QInstruments production takes place in Germany.
The focus is on human diligence and on being environmentally friendly. The company upholds its quality guarantee by means of consistent quality management. For more than 15 years these lab automation professionals from Jena have used only high-quality materials to ensure sustainable production, applied innovative thinking and undertaken research in a future-oriented way – all as a matter of course.
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Ideal for microplates & PCR plates High-precision mixing from 200 up to 3,000 rpm Universal spring clamps for easy fixation Wide range of adapters Stylish, fire resistant aluminium housing
m-PEG6-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
Document
m-PEG6-DBCO is a monodisperse PEG reagents which can enable copper-free Click Chemistry through the reaction of DBCO with azide. Reagent grade, for research purpose. Please contact us for GMP-grade inquiries.
N-(Propargyl-PEG2)-DBCO-PEG3-N-Boc enables formation of triazole linkage with azide-bearing compound via copper catalyzed Click Chemistry. Under mild acidic conditions, t-Boc group can be removed to yield the free amine. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose.
Document
N-(Propargyl-PEG2)-DBCO-PEG3-N-Boc enables formation of triazole linkage with azide-bearing compound via copper catalyzed Click Chemistry. Under mild acidic conditions, t-Boc group can be removed to yield the free amine. The propargyl group can be linked to azide-containing biomolecules via Click Chemistry. Reagent grade, for research purpose.
